Nucleic Acid Amplification Techniques
Detection of Rhinoviruses by Tissue Culture and Two Independent Amplification Techniques, Nucleic Acid Sequence Based Amplification and Reverse Transcription PCR, in Children with Acute Respiratory Infections during a Winter Season
... and nucleic acid amplification techniques—nucleic acid sequence-based amplification (NASBA) and re- verse transcription-PCR (RT-PCR)—were applied to detect rhinoviruses ...
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International survey to identify diagnostic needs to support malaria elimination: guiding the development of combination highly sensitive rapid diagnostic tests
... The WHO currently recommends malaria diagnosis either by microscopy or rapid diagnostic test (RDTs) in patients with suspected malaria prior to treatment [1]. Conventional RDTs are adequate for diagnosing people with ...
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Comparison of clinical performance of antigen basedenzyme immunoassay (EIA) and major outer membrane protein (MOMP)-PCR for detection of genital Chlamydia trachomatis infection
... several nucleic acid amplification techniques and polymerase chain reaction (PCR)-based methods have become available which provide improved sensitivity for diagnosis of ...molecular ...
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Journal of Applied Pharmaceutical Science
... on nucleic acid amplification technologies (NAATs) in pharmaceutical product ...available nucleic acid and gene amplification-based rapid microbiological methods has increased ...
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Role of cbnaat (cartridge based nucleic acid amplification test) in the diagnosis of tubercular pleural effusion
... on Nucleic acid amplification techniques (NAAT) (Pai and Ling, ...based nucleic acid amplification test (CBNAAT), specific for Mycobacterium tuberculosis has been recently ...
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Urine Specimens from Pregnant and Nonpregnant Women Inhibitory to Amplification of Chlamydia trachomatis Nucleic Acid by PCR, Ligase Chain Reaction, and Transcription Mediated Amplification: Identification of Urinary Substances Associated with Inhibition and Removal of Inhibitory Activity
... Nucleic acid amplification (NAA) techniques such as PCR, ligase chain reaction (LCR), and transcription-mediated am- plification (TMA) have greatly improved our ability to diag- nose Chlamydia ...
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High Throughput Detection of West Nile Virus RNA
... sensitive nucleic acid-based techniques, including nucleic acid se- quence-based amplification (NASBA) (6) and branched DNA (bDNA) methods, are available for development for WNV ...
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Results: Smear positive cases CBNAAT positivity was 95.91% and smear negative cases 10.48%.
... Molecular techniques have revolutionized the diagnosis of TB, MDR ...automated Nucleic acid amplification test, which is extremely helpful in early diagnosis and to initiate the treatment of ...
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Improved Sensitivity of Nucleic Acid Amplification for Rapid Diagnosis of Tuberculous Meningitis
... diagnostic techniques are insensitive, slow, or ...displacement amplification method (the standard ProbeTec method) for the detection of Mycobacterium tuberculosis complex organisms in parallel with the ...
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Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real Time Reverse Transcription Loop Mediated Isothermal Amplification Assay
... Molecular techniques based on genomic sequence detection by reverse transcription (RT)-PCR, nested PCR, nucleic acid sequence-based amplification (NASBA), and real-time PCR are therefore ...
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Prevalence of Plasmodium spp in malaria asymptomatic African migrants assessed by nucleic acid sequence based amplification
... other techniques have been developed, such as immunochromatographic assays based on Plasmodium antigen detection, but some of these tests perform poorly in cases of low parasitemia [7,9,10] or, in contrast, lead ...
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Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis
... of nucleic acid compared to other tests, including ...for amplification are added to the reaction mixture in a special designated room that is considered to be free of ...
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Application of serial tests for Mycobacterium tuberculosis detection to active lung tuberculosis cases in Indonesia
... and nucleic acid ...a nucleic ampli- fication test, with a sensitivity of ...the amplification of nucleic acid similarly yielded positive results for sam- ples that had negative ...
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A formalin free method for stabilizing cells for nucleic acid amplification, hybridization and next generation sequencing
... acetic acid fixative was added to the cells and incubated at room temperature for 5 min before centrifugation at 1200 rpm for 6 min to remove hypotonic/fixative ...
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Prospective study to find out the role of gastric aspirate examination by Ziehl-Neelsen staining (ZN staining) and cartridge based nucleic acid amplification test (CB-NAAT) as a diagnostic method in childhood tuberculosis
... This test is widely anticipated to replace smear microscopy in resource-poor settings where HIV co- infection or drug-resistant tuberculosis are common, and present results suggest that its use is a major improvement ...
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A Case of Ankle Osteomyelitis Caused by Mycobacterium abscessus
... It is not easy to distinguish osteomyelitis caused by NTM from infection due to Mycobacterium tuberculosis, resulting in an average time span for diagnosis of 10 months or longer. The presented case took 52 days for a ...
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Impact of the introduction of a nucleic acid amplification test for Clostridium difficile diagnosis on stool rejection policies
... To overcome this problem, nucleic acid amplification tests (NAATs) have been developed and are increasingly adopted. They are reported to have sensitivities ranging from 84 to 94% and short ...
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Development of Real Time Multiplex Nucleic Acid Sequence Based Amplification for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp in Respiratory Specimens
... (16). Nucleic acids were extracted as described by Boom et ...The nucleic acid-silica complex was washed twice with guanidinium thiocyanate washing solution, twice with 70% (vol/vol) ethanol, and ...
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Trichomoniasis and associated co-infections of the genital tract among pregnant women presenting at two hospitals in Ghana
... Deoxyribonucleic Acid; GNRs: Gram Negative Rods; HBV: Hepatitis B Virus; HIV: Human Immunodeficiency Virus; NAAT: Nucleic Acid Amplification Technique; pH: Power of hydrogen; PID: Pelvic ...
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An Analysis of Lymphadenopathy Cases with reference to Tuberculosis by Combined Results of Conventional and Molecular Methods: A Study in a Tertiary Care Pediatric Institute
... for acid fast bacilli by Ziehl-Neelsen method and the third and final portion was transferred into a conical sterile test tube (Falcon Tube) containing sterile normal saline and the needle was thoroughly rinsed ...
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