nucleic acid isothermal amplification
Loop Mediated Isothermal Amplification Method for Rapid Detection of the Periodontopathic Bacteria Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola
... Nucleic acid amplification is one of the most valuable meth- ods for research in the life sciences; the new technique has particularly benefited amplicon-oriented sciences, including studies ...
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Multicenter Clinical Evaluation of the Alere i Respiratory Syncytial Virus Isothermal Nucleic Acid Amplification Assay
... an isothermal nu- cleic acid amplification test capable of detecting RSV directly from respiratory speci- mens, with results being available in ⱕ 13 min after test ...plus nucleic acid test; ...
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<p>Current and Future Perspectives on Isothermal Nucleic Acid Amplification Technologies for Diagnosing Infections</p>
... why nucleic acid ampli fi cation appears to be cumbersome is the need for initial extraction of the patho- gen ’ s nucleic acid, ampli fi cation of the sequence of interest in the nucleic ...
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Evaluation of non instrumented nucleic acid amplification by loop mediated isothermal amplification (NINA LAMP) for the diagnosis of malaria in Northwest Ethiopia
... non-instrumented nucleic acid amplification loop-mediated isothermal amplification (NINA-LAMP) compared to standard thick and thin film microscopy and nested PCR as gold standard for ...
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Detection of virus mRNA within infected host cells using an isothermal nucleic acid amplification assay: marine cyanophage gene expression within Synechococcus sp
... in isothermal nucleic acid amplification is cur- rently ...addition, isothermal amplification tech- niques are potentially more robust, and have lower energy requirements than ...
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Three Isothermal Amplification Techniques for Rapid Identification of Cladophialophora carrionii, an Agent of Human Chromoblastomycosis
... Loop-mediated isothermal amplification of DNA (LAMP), a recently developed detection technique (12), may offer a cheaper alternative without compromising sensitivity and ...autocycling amplification ...
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Loop Mediated Isothermal Amplification (LAMP) Assay for GMO on: Recent Progresses and Future Perspectives
... Loop-mediated isothermal amplification (LAMP), an isothermal nucleic acid amplification technique, has been extensively used in many areas such as food safety and clinic ...
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Quantitative Detection of Hepatitis B Virus DNA by Real Time Nucleic Acid Sequence Based Amplification with Molecular Beacon Detection
... target amplification resulting in RNA ...one-tube, isothermal nature of the method, which allows high-throughput applications for nucleic acid ...
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Nucleic Acid Sequence Based Amplification (Nasba)-Prospects And Applications
... (MAP) (Rodríguez-Lázaro et al. 2004a) was very disappointing. It would have been very advantageous to have a rapid NASBA-based viability assay. Other organisms for which a rapid viability assay would be useful include ...
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Duplex Molecular Assay Intended for Point of Care Diagnosis of Influenza A/B Virus Infection
... utilizes isothermal amplification and visual dipstick detection of viral nucleic acid (amplification product) to further reduce the complexity of molecular ...
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Detection of nucleic acid of classical swine fever virus by reverse transcription loop mediated isothermal amplification (RT LAMP)
... The CSFV Bangkhen strain as reference strain, ob- tained from Department of Livestock Development, Ministry of Agriculture and Cooperatives, was used as the viral nucleic acid/positive standard in the assay ...
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Detection of Four Plasmodium Species by Genus and Species Specific Loop Mediated Isothermal Amplification for Clinical Diagnosis
... Loop-mediated isothermal amplification (LAMP), a novel nucleic acid amplification method, was developed for the clinical detection of four species of human malaria parasites: Plasmodium ...
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Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis
... study isothermal, nucleic acid sequence based amplification (NASBA) was coupled to oligochromatography (OC) to develop a simplified detection method for the diagnosis of ...
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Comparison of clinical performance of antigen basedenzyme immunoassay (EIA) and major outer membrane protein (MOMP)-PCR for detection of genital Chlamydia trachomatis infection
... This study emphasizes the need for implementation of a routine C. trachomatis screening in public health programs for women particularly those with cervicitis. Since long term C. trachomatis infection may result in ...
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Identification of human immunodeficiency virus sequences by using in vitro enzymatic amplification and oligomer cleavage detection.
... We report here the application of recently developed nucleic acid amplification and oligonucleotidebased detection procedures for the identification of HIV sequences in established infec[r] ...
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A formalin free method for stabilizing cells for nucleic acid amplification, hybridization and next generation sequencing
... acetic acid fixative was added to the cells and incubated at room temperature for 5 min before centrifugation at 1200 rpm for 6 min to remove hypotonic/fixative ...
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Improved Sensitivity of Nucleic Acid Amplification for Rapid Diagnosis of Tuberculous Meningitis
... displacement amplification method with CSF specimens prospectively collected from both countries where the incidence of TBM is low and countries where the incidence of TBM is ...
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A Case of Ankle Osteomyelitis Caused by Mycobacterium abscessus
... tuberculosis nucleic acid amplification tests and overlooking the possibility of non-tuberculous mycobacterial infection are thought to be reasons for treatment ...tuberculosis nucleic ...
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Nucleic Acid Amplification Assays for Detection of La Crosse Virus RNA
... these nucleic acid amplification assays provide an ad- vantageous combination of sensitivity, specificity, and ...with nucleic acid amplification assay-positive–live virus ...
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Considerations on the use of nucleic acid based amplification for malaria parasite detection
... the enzymes or reagents, the type of thermocycler used, and/or subtle variations in the manner in which template preparation was carried out. Although, the main conse- quence of this variation was a modest reduction in ...
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