Nucleus pulposus-like differentiation
Differentiation of human induced pluripotent stem cells into nucleus pulposus-like cells
... stepwise differentiation protocol was developed to generate NP-like cells through an early inductive stage of mesodermal and then notochordal lineages; followed by cell expansion and pellet culture ...
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Angiopoietin-1 receptor Tie2 distinguishes multipotent differentiation capability in bovine coccygeal nucleus pulposus cells
... primary nucleus pulposus cells (NPC) from bovine coccygeal discs and sorted these for the Tie2 marker, where the Tie2+ fraction of cells is suggested to represent the nucleus pulposus ...
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Novel immortal human cell lines reveal subpopulations in the nucleus pulposus
... IVD- like differentiation of MSCs ...used differentiation conditions that have previously been established for AC ...cell differentiation are currently unknown, some caution is warranted in ...
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Co-culturing nucleus pulposus mesenchymal stem cells with notochordal cell-rich nucleus pulposus explants attenuates tumor necrosis factor-α-induced senescence
... multilineage differentiation tests, all cells were capable of osteogenic, chondrogenic, and adipogenic differentiation in the respective types of differentiation induction media after 3 weeks ...
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Growth differentiation factor 6 and transforming growth factor beta differentially mediate mesenchymal stem cell differentiation, composition, and micromechanical properties of nucleus pulposus constructs
... driving differentiation more toward a chondrocyte-like ...cogenic differentiation of both BM-MSCs and AD-MSCs, with gene-expression changes being greatest in AD-MSCs, suggesting that these cells may ...
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Notochordal cell conditioned medium stimulates mesenchymal stem cell differentiation toward a young nucleus pulposus phenotype
... MSC differentiation down a lineage different from standard mesenchymal ...MSC differentiation offers ...the differentiation profile of the MSCs, collagen III and laminin isoforms may provide useful ...
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Nucleus pulposus phenotypic markers to determine stem cell differentiation : fact or fiction?
... A multitude of proposed genes have been identified as NP phenotypic markers, those which are less well validated include the OVO-2[10], FOXF1 [10, 15] and PAX1 [10, 15]. Additionally LAM-332 (LAM-5) and LAM-511 (LAM-10) ...
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Exosomes as potential alternatives to stem cell therapy for intervertebral disc degeneration: in-vitro study on exosomes in interaction of nucleus pulposus cells and bone marrow mesenchymal stem cells
... degenerate IVD aim at increasing the number of vi- able cells capable of producing an appropriate and functional NP-like matrix. The main type of stem cells in application for degenerate NP restoration is MSCs, ...
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CD24 identifies nucleus pulposus progenitors/notochordal cells for disc regeneration
... treatment of spinal disorders [16]. Progenitor cells do have an advantage over terminally differentiated cells in that the progenitors maintain their multipotent differen- tiation and self-renewal potential in vivo and ...
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Original Article Differentiation of immortalized human precartilaginous stem cells into nucleus pulposus-like cells
... In conclusion, we have shown that IPSCs can be differentiated into NP-like cells follow- ing induction by TGF-β 1 . This preliminary study provides a basis for trialing stem cell- mediated cell transplantation of ...
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Original Article Development of a KLD-12 polypeptide/TGF-β1-tissue scaffold promoting the differentiation of mesenchymal stem cell into nucleus pulposus-like cells for treatment of intervertebral disc degeneration
... New Zealand white rabbits were anesthetized by injecting chloral hydrate (10%) intravenou- sly in the ear vein. Approximately 4 ml of bone marrow was extracted under sterile condi- tions through the femoral bone and the ...
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Original Article Intermediate-term clinical effects of Dynesys dynamic stabilization system on double-segmental lumbar disc herniation
... and nucleus pulposus signal intensity of adjacent segments were measured by imaging examination; logistics regression analysis was used to analyze the related risk factors of adjacent segment degeneration ...
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Degeneration Affects the Structural and Tissue Mechanics of the Intervertebral Disc
... specimens. The short study in Chapter 5 demonstrated very little differences at the mid- sagittal section of the disc following annulotomy at the posterior-lateral annulus fibrosus. A common cause for low back pain is ...
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Spatial geometric and magnetic resonance signal intensity changes with advancing stages of nucleus pulposus degeneration
... each nucleus pulposus on the mid-sagittal images, and to locate the coordinates of the geometric and weighted centers of the nucleus ...the nucleus pulposus by calculating the distance ...
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Inter- and intraobserver agreement for diagnosing presumptive ischemic myelopathy and acute noncompressive nucleus pulposus extrusion in dogs using magnetic resonance imaging
... Fenn, J., Drees, R., Volk, H. A. and Decker, S. D. (2016), INTER- AND INTRAOBSERVER AGREEMENT FOR DIAGNOSING PRESUMPTIVE ISCHEMIC MYELOPATHY AND ACUTE NONCOMPRESSIVE NUCLEUS PULPOSUS EXTRUSION IN DOGS USING ...
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Intradiscal transplantation of synovial mesenchymal stem cells prevents intervertebral disc degeneration through suppression of matrix metalloproteinase related genes in nucleus pulposus cells in rabbits
... of nucleus pulposus, and Chondroitin sul- fate proteoglycan 2, a member of the hyaluronan-binding proteoglycan family, were significantly up-regulated, though Aggrecan 1, another principal component of ...
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0 6 T MR imaging of the cervical spine: multislice and multiecho techniques
... Cord compression associated with spinal stenosis, as well as extradural defects associated with cervical spondylosis, herniated nucleus pulposus, metastases, and postoperative changes, i[r] ...
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Sensitivity analysis of permeability parameters of bovine nucleus pulposus obtained through inverse fitting of the nonlinear biphasic equation : effect of sampling strategy
... Computer Methods in Biomechanics and Biomedical Engineering Sensitivity analysis of permeability parameters of bovine nucleus pulposus obtained through inverse fitting of the nonlinear b[r] ...
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Original Article The role of lncRNA MALAT1 in intervertebral degenerative disc disease
... Intervertebral disc degeneration disease (IDDD) is a common clinical disease. As a common bone disease, it can lead to neck and shoulder pain which brings severe problem to the global health [1, 2]. Chronic low back pain ...
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Osseous erosion by herniated nucleus pulposus mimicking intraspinal tumor: a case report
... Increased intraspinal pressure exerted by an expanding mass such as a slow-growing intraspinal tumor is a common cause of erosion (scalloping) of the posterior vertebral wall [1–8]. As for other causes of scalloping, ...
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