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ORF-2

A herpesvirus saimiri membrane protein required for interleukin-2 independence forms a stable complex with p56lck.

A herpesvirus saimiri membrane protein required for interleukin-2 independence forms a stable complex with p56lck.

... about 2 months (5, ...proteins: ORF-1 and ORF-2 (16, ...either ORF-1 or ORF-2 abolishes the expres- sion of an IL-2-independent phenotype of infected T cells, ...

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Open Reading Frame 2, Encoded by the Latency-Related Gene of Bovine Herpesvirus 1, Has Antiapoptotic Activity in Transiently Transfected Neuroblastoma Cells

Open Reading Frame 2, Encoded by the Latency-Related Gene of Bovine Herpesvirus 1, Has Antiapoptotic Activity in Transiently Transfected Neuroblastoma Cells

... Tag2. ORF-2 sequences derived from cDNA at 1 or 15 dpi were cloned into a unique BamHI-HindIII site, pCMV-Tag2A, pCMV-Tag2B, or pCMV-Tag2C (Stratagene, La Jolla, CA), in order to obtain the re- spective ...

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Asynchronous Accumulation of Lettuce Infectious Yellows Virus RNAs 1 and 2 and Identification of an RNA 1 trans  Enhancer of RNA 2 Accumulation

Asynchronous Accumulation of Lettuce Infectious Yellows Virus RNAs 1 and 2 and Identification of an RNA 1 trans Enhancer of RNA 2 Accumulation

... RNA 2 (pSP6) (11) were used for mutagenesis when ...of ORF 1B and ORF 2 (encoding P32) in pEco ...and 2 nucleotides, respectively, into ORF 2 of P32 F1 and P32 F2, ...

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Norwalk Virus Open Reading Frame 3 Encodes a Minor Structural Protein

Norwalk Virus Open Reading Frame 3 Encodes a Minor Structural Protein

... The ORF 2 coding region was PCR amplified from pUCNV-4145 (26) using primers NV-110a (5⬘-GCGGCGAGA TCTAATTCGTAAATGATGATGGCG-3⬘; nucleotides [nt] 5355 to 5374 [BglII site underlined]) and NV-111a ...

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Expression, characterization, and immunoreactivities of a soluble hepatitis E virus putative capsid protein species expressed in insect cells.

Expression, characterization, and immunoreactivities of a soluble hepatitis E virus putative capsid protein species expressed in insect cells.

... HEV ORF-2 protein species expressed in insect cells, (ii) to identify the most promising candidate in terms of immunoreactivity, and (iii) to design a process to express this candidate protein in a ...

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Serological Immunoassay for Detection of Hepatitis E Virus on the Basis of Genotype 3 Open Reading Frame 2 Recombinant Proteins Produced in Trichoplusia ni Larvae

Serological Immunoassay for Detection of Hepatitis E Virus on the Basis of Genotype 3 Open Reading Frame 2 Recombinant Proteins Produced in Trichoplusia ni Larvae

... complete ORF-2, which occurs by truncation of the N terminus of the protein (15, 26, ...full-length ORF-2 in Sf21 cells and ...HEV ORF-2 protein expressed in suspension versus ...

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Molecular Characterization of a Bovine Enteric Calicivirus: Relationship to the Norwalk-Like Viruses

Molecular Characterization of a Bovine Enteric Calicivirus: Relationship to the Norwalk-Like Viruses

... times with RIPA buffer and with a final wash in PBS before derivitization in sample-dissociating buffer and separation by SDS-PAGE. pJVC1 used as a template in a coupled transcrip- tion-translation reaction gave a single ...

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Tracing Evolutionary and Developmental Implications of Mitochondrial Stoichiometric Shifting in the Common Bean

Tracing Evolutionary and Developmental Implications of Mitochondrial Stoichiometric Shifting in the Common Bean

... room temperature for 3–5 days. Primary roots of ⵑ2 cm in Plant materials: To assess the frequency of pvs-orf 239 in P. length were used. Two sections within the meristematic zone vulgaris populations, a ...

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Severe Acute Respiratory Syndrome Coronavirus Open Reading Frame (ORF) 3b, ORF 6, and Nucleocapsid Proteins Function as Interferon Antagonists

Severe Acute Respiratory Syndrome Coronavirus Open Reading Frame (ORF) 3b, ORF 6, and Nucleocapsid Proteins Function as Interferon Antagonists

... SARS-CoV ORF 3b, ORF 6, and N proteins as interferon ...proteins—the ORF 3b, ORF 6, and N proteins—rescued the growth of NDV- GFP, indicating that they are potential interferon ...since ...

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Targeted Genome Sequencing Reveals Varicella-Zoster Virus Open Reading Frame 12 Deletion

Targeted Genome Sequencing Reveals Varicella-Zoster Virus Open Reading Frame 12 Deletion

... of ORF 12 but removed the coding sequence for the remaining C-terminal 211 amino ...truncated ORF 12 was generated from a fusion of TAC (tyrosine codon) of ORF 12 with the ATG (initiation codon) of ...

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Molecular characterization of Orf virus in goats in Gabon, Central Africa

Molecular characterization of Orf virus in goats in Gabon, Central Africa

... In rural areas of Gabon, small ruminants are bred by straying and frequent contacts between humans and lambs or kids, which are highly susceptible to orf infec- tions, could lead to human infection, particularly ...

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cDNA Cloning and Overexpression of Acidic Ribosomal Phosphoprotein P1 Gene (RPLP1) from the Giant Panda

cDNA Cloning and Overexpression of Acidic Ribosomal Phosphoprotein P1 Gene (RPLP1) from the Giant Panda

... PCR fragment corresponding to the RPLP1 polypeptide was amplified from the RPLP1 cDNA clone with the forward primer, 5’-TCT GGA TCC CTC GCC CGC ACC ATG -3’ (BamHI) and reverse primer, 5’- CTC AAG CTT TTA GTC AAA AAG ACC ...

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Coupled Translation of the Second Open Reading Frame of M2 mRNA Is Sequence Dependent and Differs Significantly within the Subfamily Pneumovirinae

Coupled Translation of the Second Open Reading Frame of M2 mRNA Is Sequence Dependent and Differs Significantly within the Subfamily Pneumovirinae

... second ORF, we have shown that the levels of expression from the second ORFs of the pneu- movirus M2 mRNA differ ...M2-2 ORF was indeed accessed by coupled translation but that this was approximately ...

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A 100-kilodalton polypeptide encoded by open reading frame (ORF) 1b of the coronavirus infectious bronchitis virus is processed by ORF 1a products.

A 100-kilodalton polypeptide encoded by open reading frame (ORF) 1b of the coronavirus infectious bronchitis virus is processed by ORF 1a products.

... Secondly, expression of plasmid DNA pIBV11 covering IBV sequence from nucleotides 8693 to 16980 in Vero cells by using a vaccinia virus-T7 expression system resulted in the synthesis of [r] ...

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Molecular characterization of orf virus from sheep and goats in Ethiopia, 2008–2013

Molecular characterization of orf virus from sheep and goats in Ethiopia, 2008–2013

... first orf outbreak investigated in this study occurred in November 2008, in sheep reared at the Adami tulu agricul- ture research center (ATARC) causing specific lesions ...

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A homolog of interleukin-10 is encoded by the poxvirus orf virus.

A homolog of interleukin-10 is encoded by the poxvirus orf virus.

... Northern blot analysis was conducted with early RNA de- rived from bovine testis cells infected with either OV strain NZ2 or OV strain NZ7. Cells were infected with virus in the presence of cycloheximide in order to ...

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Thymic Lesions in Cats Infected with a Pathogenic Molecular Clone or an ORF-A/2-Deficient Molecular Clone of Feline Immunodeficiency Virus

Thymic Lesions in Cats Infected with a Pathogenic Molecular Clone or an ORF-A/2-Deficient Molecular Clone of Feline Immunodeficiency Virus

... cats. ORF-A/2 deficiency delays the reduction of the peripheral blood CD4/CD8 ...an ORF-A/2-deficient molec- ular clone to reduce the peripheral blood CD4/CD8 ratio of infected ...(Fig. ...

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Bacillus anthracis Virulent Plasmid pX02 Genes Found in Large Plasmids of Two Other Bacillus Species

Bacillus anthracis Virulent Plasmid pX02 Genes Found in Large Plasmids of Two Other Bacillus Species

... Sequencing. Different combinations of forward and reverse primers were tested to determine which pairs produced large amplicons that could be used as a template for the sequencing PCRs (Tables 2 and 3 and Fig. 1). ...

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Sequence comparison of porcine respiratory coronavirus isolates reveals heterogeneity in the S, 3, and 3-1 genes.

Sequence comparison of porcine respiratory coronavirus isolates reveals heterogeneity in the S, 3, and 3-1 genes.

... mRNA 2 (27). The ORF 3 and ORF 3-1 genes of the Miller strain of TGEV are found at the 5 9 end of two separate subgenomic mRNAs, 3 and 3-1, respectively, and are thought to encode two putative ...

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Modulation of Human Herpesvirus 8/Kaposi's Sarcoma-Associated Herpesvirus Replication and Transcription Activator Transactivation by Interferon Regulatory Factor 7

Modulation of Human Herpesvirus 8/Kaposi's Sarcoma-Associated Herpesvirus Replication and Transcription Activator Transactivation by Interferon Regulatory Factor 7

... the ORF 57 RRE suggests that two RTA molecules can bind to the RRE either as a dimer or as two separate ...this ORF 57 RRE ...the ORF 57 promoter RRE region will result in strong activation of ...

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