PCR and cloning of PCR products
Five-minute cloning of Taq polymerase-amplified PCR products
... Representatives. Invitrogen warrants that all of its products will perform according to the specifications stated on the certificate of analysis. The company will replace, free of charge, any product that does not ...
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CHAPTER 6. CLONING AND SEQUENCING OF CCCVd-RELATED RT-PCR PRODUCTS
... The occurrence of these variants agrees with the RPA analysis (Chapter 4), which showed that the CCCVd-like RNAs in this palm had several mismatches when compared with CCCVd. The presence of sequences equivalent to OP ...
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Five-minute cloning of Taq polymerase-amplified PCR products for sequencing
... components, whether or not such product or its components are resold for use in research. Invitrogen Corporation will not assert a claim against the buyer of infringement of the above patents based upon the manufacture, ...
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Easy-A High-Fidelity PCR Cloning Enzyme
... High-Fidelity PCR Cloning Enzyme 3 I NTRODUCTION The Easy-A high-fidelity PCR cloning enzyme* is a proprietary thermostable DNA polymerase formulation specifically designed for improved ...
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TA PCR Cloning Kit (ptakn-2)
... of PCR products into plasmid. The procedure is fast and easy. PCR products are simply mixed with pTAKN-2 Vector, 2 × Ligation Buffer and Ligase mixture, then incubated at 16℃ for 30 ...
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TA PCR Cloning Kit (ptac-2)
... The cloning of PCR products into plasmids is a routine step in many molecular biology ...TA PCR Cloning Kit is a PCR cloning kit based on T-A pairing of PCR ...
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5PCR, real-time PCR, reverse transcription, and cloning
... Higher PCR specificity and reduced non-specific amplification ■ Optional ready-to-load buffer additive for easier handling Product description HotStarTaq Plus Master Mix contains HotStarTaq Plus DNA Polymerase, ...
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PCR Reagents. The Highest Level of PCR Performance. PCR Enzymes RT-PCR Reagents QPCR and QRT-PCR Reagents dntps
... high-fidelity PCR cloning enzyme does not require any post-PCR A-addition ...Thus, PCR products generated with the Easy-A PCR cloning enzyme can be added directly to T- or ...
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LUMI-PCR: an Illumina platform ligation-mediated PCR protocol for integration site cloning, provides molecular quantitation of integration sites
... all PCR products can be used as template for the next ...the PCR products during the secondary PCR ...2° PCR primers per plate arranged so that no indexed primer is adjacent to ...
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Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy
... recombination PCR. As a result, all validation experi- ments cloning DNA fragments with different length suc- ceeded without any optimization of the recombination PCR ...
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Plasmid DNA, Gel Extraction & PCR Products Purification Kits. Code Description Size. BS363 EZ-10 Spin Column PCR Products Purification Kit 50 preps
... of PCR products and other DNA fragments from gel extraction solutions and enzymatic ...procedure. PCR products or other enzymatic reactions are mixed with a specialized binding buffer and pass ...
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Efficient Cloning of Alternatively Polyadenylated Transcripts via Hybridization Capture PCR
... Abstract Cloning of alternatively polyadenylated transcripts is crucial for studying gene expression and ...positive products due to the usage of a single gene specific ...
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Amplification Products for PCR and RT-PCR
... Epidermal Growth Factor Receptor, Primer Set Plus Positive Control T59490. ENA-78 Primer Set Plus Positive Control T59500[r] ...
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pcr 8/GW/TOPO TA Cloning Kit
... your PCR product into a Gateway ® destination vector for downstream expression studies, remember to include the sequences required for proper translation initiation and termination of your PCR ...your ...
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Cloning of DNA fragment amplified by PCR method into plasmid vector
... provedbe PCR-a postoji nekoliko stavki koje se moraju uzeti u ...odvijanja PCR-a pri preniskoj temperaturi denaturacije. Nadalje, na uspješnost PCR reakcije utječe koncentracija enzima DNA ...
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Real Time PCR Tests Short Overview PCR Products - Switzerland / Benelux 2021
... Applied Biosystems 7300 / 7500, CFX Connect™ / CFX96™/ Dx, LightCycler ® 480, AriaMx Real-Time PCR System, LineGene 9600 Plus, Mic qPCR Cycler Rotor-Gene ® 3000 / 6000 / Q, SLAN ® [r] ...
Troubleshooting for PCR and multiplex PCR
... unspecific products for each locus tested with the unspecific products seen when running the multiplex ...unspecific products in the multiplex ...
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PCR & DNA Sequencing. PCR= Polymerase Chain Reaction. PCR applications
... of PCR reaction: • As a rule of thumb, use an annealing temperature (Ta) about 5 o C below the lowest Tm of the pair of primers to be used if a good yield of product is ...
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Robust LCA: PCR guide for construction products and works
... generic PCR for all construction works that is precise enough to enable two independent LCA practitioners to perform equal LCAs for a specific construction work covering the full life ...
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PCR Guidance-Texts for Building-Related Products and Services
... The products, plants and their locations must be outlined, on which data the Life Cycle Assessment is based and for which the declaration applies. For average EPDs, e.g. association EPDs, the plants/companies ...
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