PCR and genotypic
Detection of Hepatitis B Virus Genotypic Resistance Mutations by Coamplification at Lower Denaturation Temperature PCR Coupled with Sanger Sequencing
... HBV genotypic resis- tance mutations, but they usually have poor sensitivity, are very expensive and complicated to use, and detect only single muta- ...HBV genotypic resistance mu- tations (at rt positions ...
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Genotypic Analyses of Vibrio parahaemolyticus and Development of a Pandemic Group Specific Multiplex PCR Assay
... Our PFGE analysis of V. parahaemolyticus strains revealed that the tdh-positive strains of O3:K6, O4:K68, O1:K25, O1: K26, and O1:KUT isolated since 1996 (collectively referred to as the “pandemic group”) were all within ...
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Genotypic Analysis of Staphylococcus aureus Coagulase Gene using PCR-RFLP analysis
... distinct PCR amplification of this region and lead to generation of DNA fragments of different sizes, which could be discriminated further by digestion with restriction enzymes such as AluI ...describe ...
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High genotypic diversity and a novel variant of human cytomegalovirus revealed by combined UL33/UL55 genotyping with broad range PCR
... represent genotypic variants of a single species, and HCMV genotypic variability has been studied in order to reveal correlations between different disease patterns and the presence of certain HCMV ...
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Genotypic Analysis of Invasive Streptococcus pneumoniae from Mali, Africa, by Semiautomated Repetitive Element PCR and Pulsed Field Gel Electrophoresis
... Repetitive-element PCR (rep-PCR) was described in 1993 as a rapid method for strain typing that relies on amplification of the sequences between various repetitive ele- ments interspersed throughout the ...
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Direct genotypic detection of Mycobacterium tuberculosis rifampin resistance in clinical specimens by using single tube heminested PCR
... first-round PCR and the aerosol-prone addition of the second-round reagents. When PCR was repeated with Ampliwax to contain the first-round products ...remained PCR positive and all of the ...
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Genotypic detection of rifampicin and isoniazid resistant Mycobacterium tuberculosis strains by DNA sequencing: a randomized trial
... antituberculous. Genotypic analysis was done only for rifampicin resistant specimens (23 cases) and INH resistant specimens (26 cases) to detect mutations responsible for drug resistance by PCR ...
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Analysis for a Limited Number of Gene Codons Can Predict Drug Resistance of Mycobacterium tuberculosis in a High Incidence Community
... criteria. Genotypic drug resistance testing was performed by mutation analysis accord- ing to a recently described PCR-based dot blot method ...Efficient PCR amplification was confirmed by gel ...
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Genotypic Differences in Dengue Virus Neutralization Are Explained by a Single Amino Acid Mutation That Modulates Virus Breathing
... overlap-extension PCR. The resulting C-prM-E PCR fragments were cloned into a mammalian expression vector using either Gateway (Life Technologies, Carlsbad, CA) or In-Fusion (Clontech, Mountain View, CA) ...
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Evaluation of PCR Restriction Profile Analysis and IS2404 Restriction Fragment Length Polymorphism and Amplified Fragment Length Polymorphism Fingerprinting for Identification and Typing of Mycobacterium ulcerans andM marinum
... the genotypic affiliation between these two closely related species, we performed a comparative analysis including PCR restriction profile analysis (PRPA), IS2404 restriction fragment length polymorphism ...
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Differentiation of porcine wild type lactobacilli strains, with ERIC PCR and PFGE band patterns included in polyphasic taxonomy
... Genotypic characterization was carried out by PFGE. Production of agarose plugs and bacterial lysis were performed as previously described (Roy et al., 1996). Bacterial DNA plugs were washed once with Tris-EDTA ...
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Phenotypic and genotypic characterization of Staphylococcus aureus isolates in milk from flocks diagnosed with subclinical mastitis.
... The characterization of the genetic profile and the clonal relationship among the S. aureus isolates were performed using the RAPD-PCR technique. For the RAPD-PCR, oligonucleotide S232 was used and the ...
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<p>Molecular characterization, serotypes and phenotypic and genotypic evaluation of antibiotic resistance of the<em> Klebsiella pneumoniae</em> strains isolated from different types of hospital-acquired infections</p>
... all PCR reac- tions. PCR grade water and ...of PCR product were exposed to electrophoresis in a 2% agarose gel in 1× TBE buffer at 80 V for 30 min, stained with SYBR ...
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BLMH and APOE genes in Alzheimer Disease: A possible relation
... the genotypic and allele frequencies of the APOE gene and BLMH 1450 G > A polymorphism and assess BLMH expression using PCR-RFLP and RT-qPCR analyses of blood samples from patients with Alzheimer dis- ...
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Serologic and genotypic characterization of hepatitis B virus in HIV 1 infected patients from South West and Littoral Regions of Cameroon
... Serologic and genotypic characterization of hepatitis B virus in HIV 1 infected patients from South West and Littoral Regions of Cameroon RESEARCH Open Access Serologic and genotypic characterization[.] ...
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Neppiness in an Introgressed Population of Cotton: Genotypic Variation and Genotypic Correlation
... Genotypic correlation was also analyzed among AFIS-measured properties and other traits including lint yield and fiber properties measured by single-instruments, i.e., stelometer, fibrograph, micronaire, etc. ...
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Evaluation of Groundnut (Arachis hypogaea L.) Genotypes for Quantitative Character & Yield Contributing Traits Satyanarayan Patidar1, Prashant Kumar Rai 2, Arvind Kumar3
... The genotype ICG 8567 identified as best genotype for pod yield kernel yield, seed yield per plant and pod yield per plant, seed yield per plant, seed index, seed yield per plant recorded high estimates of ...
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A Systematic Review and Meta analysis of the Diagnostic Accuracy of Nucleic Acid Amplification Tests for Tuberculous Meningitis
... Diagnostic method Conventional PCR Nested PCR Nested PCR Conventional PCR Conventional PCR Multiplex PCR Multiplex PCR Multiplex PCR Nested PCR Nested PCR Conventional PCR Conventional P[r] ...
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Send more data: a systematic review of mathematical models of antimicrobial resistance
... Validation is an essential factor when developing a mathematical model. Validation can be both internal (conducted to ensure that the model is doing what it should) and external (conducted to assess whether the model ...
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Bias and Sampling Error of the Estimated Proportion of Genotypic Variance Explained by Quantitative Trait Loci Determined From Experimental Data in Maize Using Cross Validation and Validation With Independent Samples
... M OLECULAR markers are used by a great number fied: (1) QTL positions are estimated with high preci- of researchers to study quantitative traits of agro- sion to choose markers showing a minimum of recombi- nomic ...
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