PCR/hybridization
Detection of enteroviruses and rhinoviruses in clinical specimens by PCR and liquid phase hybridization
... enterovirus PCR-hybridization were expected because of sequence homol- ogy between these two picornavirus ...enterovirus PCR- hybridization tests but reacted in their enterovirus ...
6
Detection of Human Herpesvirus 6 DNA in Serum by a Microplate PCR Hybridization Assay
... Detection of amplified products. Amplified products were detected by a com- mercially available liquid hybridization-based microtiter plate assay (PCR ELISA; Boehringer Mannheim). The manufacturer’s ...
5
Comparison of Cultivation and PCR Hybridization for Detection ofSalmonella in Porcine Fecal and Water Samples
... the PCR detection of Salmonella, a 1-ml portion was removed from each sample after the first overnight enrichment and the DNA was extracted using a Sepharose CL-6B spin ...Southern hybridization with a ...
8
Panfungal PCR and Multiplex Liquid Hybridization for Detection of Fungi in Tissue Specimens
... ITS PCR amplified fungal DNA in 19 (95%) of 20 tissue ...diagnostic PCR products were ...specific hybridization (6, 8, ...ITS PCR when compared with culture and microscopic ...and PCR, ...
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PCR Reverse Blot Hybridization Assay for Screening and Identification of Pathogens in Sepsis
... For PCR-REBA, hybridization of the PCR products with probes immobilized on a membrane was carried out according to the manufacturer’s ...denatured PCR products in hybrid- ization solution (HS) ...
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Detection and Genotyping of Human Rotavirus VP4 and VP7 Genes by Reverse Transcriptase PCR and Reverse Hybridization
... VP4 PCR was positive for more than 97% of the rotavirus antigen-positive ...Reverse hybridization yielded clear VP4 genotypes for approximately 98% of the PCR-positive samples, but all samples were ...
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Detection of Human Picornaviruses by Multiplex Reverse Transcription PCR and Liquid Hybridization
... The assay was used to analyze clinical specimens, including 14 CSF specimens, 4 serum specimens, 5 nasopharyngeal spec- imens, and 68 stool specimens. For the CSF, serum, and na- sopharyngeal specimens, the results ...
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Differentiation of Leptospira interrogans isolates by IS1500 hybridization and PCR assays
... blot hybridization and PCR ...IS1500. Hybridization analysis showed that IS1500 was present on polymorphic fragments and that differences in these patterns could be used to identify ...serovars. ...
6
Rapid Diagnosis of Bacterial Meningitis by Real Time PCR and Fluorescence In Situ Hybridization
... Real-time PCR and fluorescence in situ hybridization (FISH) were evaluated as rapid methods for the diagnosis of bacterial meningitis and compared to standard diagnostic ...For PCR, a LightCycler ...
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Comparison of Real Time PCR Signal Amplified In Situ Hybridization and Conventional PCR for Detection and Quantification of Human Papillomavirus in Archival Cervical Cancer Tissue
... real-time PCR is not yet a standard diagnostic method in many laboratories, in particular for the quantifi- cation of human papillomavirus ...real-time PCR to that of chromogenic tyramide–signal-amplified ...
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Specific Detection and Identification of Herpes B Virus by a PCR Microplate Hybridization Assay
... Application to seropositive monkey specimens. PCR-micro- plate hybridization was applied to the trigeminal ganglia of four seropositive cynomolgus monkeys. Amplified products of the C region were detected ...
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Detection of Ureaplasma urealyticum by PCR and Biovar Determination by Liquid Hybridization
... Liquid hybridization. (i) Sequencing. The urease gene amplicons from the 14 serotype standard strains were produced by PCR with the primers U5 and U4 and were subsequently sequenced by cycle sequencing. The ...
6
Comparative study of bacteriological culture and real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay in the diagnosis of bacterial neonatal meningitis
... Two PCR based molecular assays, real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR based-reverse line blot hybridization (mPCR/RLB), were used to assess 7 common ...
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Detection of Pseudomonas pseudomallei by PCR and hybridization
... pseudomallei artificially inoculated into blood and sputum by PCR amplification with conserved primers PPMB2-PPMC and hybridization with a digoxigenin-labelled PPM2 probe detected with l[r] ...
7
Evaluation of Real Time PCR versus PCR with Liquid Phase Hybridization for Detection of Enterovirus RNA in Cerebrospinal Fluid
... phase hybridization assay had been shown to give false-positive results with all five of the rhinovirus serotypes (1B, 12, 15, 38, and 56) ...liquid-phase hybridization assays, comparison testing was done ...
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Efficient Cloning of Alternatively Polyadenylated Transcripts via Hybridization Capture PCR
... Cloning of alternatively polyadenylated transcripts is crucial for studying gene expression and function. Recent transcriptome analysis has mainly focused on large EST clone collections. However, EST sequencing ...
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Development of PCR based hybridization protocol for identification of streptococcal species
... tempted with nucleic acids from only three species of Strepto- coccus. However, 16S rRNA sequence data from a total of 31 species of Streptococcus were available. The PCR primers used to amplify probe target ...
6
Diagnosis of cutaneous leishmaniasis and species discrimination of parasites by PCR and hybridization
... By hybridizing the PCR product to new kDNA probes specific for either Leishmania mexicana or Leishmania braziliensis, we found that both species are major causes of cutaneous leishmanias[r] ...
7
A general primer GP5+/GP6(+) mediated PCR enzyme immunoassay method for rapid detection of 14 high risk and 6 low risk human papillomavirus genotypes in cervical scrapings
... bin PCR-positive cervical scrapings were analyzed by both HR/LR cocktail in the EIA and conventional Southern blot hybridization with a radioactively labeled general probe, de- veloped to distinguish ...
5
In situ polymerase chain reaction based localization studies support role of human herpesvirus 8 as the cause of two AIDS related neoplasms: Kaposi's sarcoma and body cavity lymphoma
... situ hybridization, since one third of their tissue samples that were PCR positive for HHV-8 were completely negative in tis- sue in situ ...situ hybridization has signifi- cant limitations for these ...
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