peptide:N-glycosidase F
Expression, purification and characterisation of recombinant peptide:N glycosidase F : a thesis presented in partial fulfilment of the requirements for the degree of Master of Philosophy in Biochemistry at Massey University
... Oligosaccharide chains attached to newly synthesised proteins in the Golgi complex may also influence the sequence of polypeptide-folding events that lead to the native tertiary structur[r] ...
239
Expression, purification and characterisation of recombinant peptide:N glycosidase F : a thesis presented in partial fulfilment of the requirements for the degree of Master of Philosophy in Biochemistry at Massey University
... The specific activity of the native PNGase Fis about four times that of the recombinant protein which may be contributed to inhibition by components of the Complete™ protease inhibitor t[r] ...
26
Maturation of Human Herpesvirus 6A Glycoprotein O Requires Coexpression of Glycoprotein H and Glycoprotein L
... the N-linked oligosaccharides on the glycoproteins are modified from high-mannose oligosaccharides into complex oli- ...and peptide-N-glycosidase F (PNGase F) digestion ...
5
Classification of K_F-orbits of Unipotent Elements in Symmetric F-varieties of SL(n, F)
... Richardson proved in 1982 that, given an algebraic group G and an involution σ, we could have only a finite number of K-orbits of unipotent elements in the symmetric variety P = G/K over an algebraically closed field, ...
152
Daisenberger, Patricia (2010): Nt-proBNP-Messung bei verschiedenen Schweregraden der hypertrophen Kardiomyopathie, anderen Kardiomyopathien sowie zur Unterscheidung kardialer und nicht kardialer Dyspnoe. Dissertation, LMU München: Tierärztliche Fakultät
... Verschiedene Faktoren können den Anstieg von BNP beeinflussen (CLERICO et al., 2002; REDFIELD et al., 2002; WANG et al., 2002). Bei herzgesunden Patienten konnte ein positiver Einfluss des weiblichen Geschlechts auf den ...
145
Peptide sequences by mass spectrometry : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University
... due to cleavage on both sides of the carbonyl group of the peptide linkage and that identification of these ions in the mass spectrum of a N-trifluoroacetyl-peptide methyl ester was suff[r] ...
159
Peptide sequences by mass spectrometry : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University
... due to cleavage on both sides of the carbonyl group of the peptide linkage and that identification of these ions in the mass spectrum of a N-trifluoroacetyl-peptide methyl ester was suff[r] ...
22
Characterization of the murine n-formyl peptide chemotactic receptor
... With the development of complementary spectrofluorometric and flow cytometric techniques, real-time analysis of the receptor in membrane preparations and whole cells can be carried out. These approaches have been used to ...
260
Absolute cross section measurements of 238U(n,f) and 237Np(n,f) in the neutron energy range 1-2.4 MeV
... An MCNP6 simulation was needed to simulate the target hall to account for all scattered neutrons that will be impinging on the samples with lower energy than expected. These neutrons will be inducing fissions in the ...
6
Dissecting conformational contributions to glycosidase catalysis and inhibition
... approximate B and S conformations; however these dif- fered from the canonical B and S conformations owing to the lack of rigidity of the ring, and the presence of attractive (hydrogen bonding) and repulsive (eclipsing ...
14
A biologically active peptide mimetic of N acetylgalactosamine/galactose
... IL-8 is a chemokine released from activated macrophages that attracts neutrophils and plays an important role in host defense by enhancing microbicidal activity and cyto- toxicity [17,18]. For example, IL-8 dramatically ...
6
Meta-analysis of B-type natriuretic peptide in diagnosis of congestive heart failure in different clinical settings
... Results: In total, 32 studies (n = 11054) met the inclusion criteria. The overall sensitivity and specificity at the optimum cutoff point are 81% (95% confidence interval: 0.76–0.86) and 86% (95% confidence ...
12
Dynamics of difference equation \(x {n+1}=f( x {n l},x {n k})\)
... Dynamics of difference equation xn+1=f(xn l,xn k)$x {n+1}=f( x {n l},x {n k})$ Moaaz Advances in Difference Equations (2018) 2018 447 https //doi org/10 1186/s13662 018 1896 0 R E S E A R C H Open Acc[.] ...
14
A functional update of the Escherichia coliK 12 genome
... We present a functional update for E. coli K-12 gene prod- ucts that incorporates information from the literature and referenced databases obtained since the 1997 GenBank deposit. Our focus has been the biological ...
7
INTESTINAL GLYCOSIDASE ACTIVITIES IN THE HUMAN EMBRYO, FETUS, AND NEWBORN
... Maltase, sucrase, isomaltase, palatinase, dextranase, lactase and cellobiase activities are distributed uniformly throughout the small intestine except in duodenum and terminal ileum. In[r] ...
13
Synthesis and α glycosidase inhibitory evaluation of isatin derivatives
... A series of different substituted isatin derivatives were designed and synthesized about 15-40% overall yields. Three compounds 3a, 3b and 3c have not been reported before. Their structures were characterized by 1 H and ...
5
Examination of tumour tissues by direct MALDI mass spectrometry imaging and profiling
... several peptide signals were observed after in situ digestion of non-treated and treated xenograft tissue sections and differences in intensities were observed when comparing profiles and ...
281
Expression of a new laccase from Moniliophthora roreri at high levels in Pichia pastoris and its potential application in micropollutant degradation
... The gene for Mrl2 (NCBI Reference Sequence: XP_007855001.1) was codon optimized (GenBank acces- sion number: KY111767) with JCat for expression in yeast (http://www.jcat.de/) and synthesized by Euro- fins (Ebersberg ...
13
Resistance of Native, Oligomeric Envelope on Simian Immunodeficiency Virus to Digestion by Glycosidases
... recognize N- glycans on the envelope proteins of these two ...the N-linked carbohydrates of free gp120 than of oligomeric ...remove N-linked carbohydrates from a membrane- associated protein and that ...
10
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... ????????? ?? ???? ???????? ?????? ???????????????????????? ?? !"?$#&%"')(?*,+ %/ 0'?*1')2?2?3 4 5656798; <79= >@?BADC?CE7F?HG I ?HGDJLKNM 8 I KO=QPRC?C?PRC SUTWV?X$Y?Z<[0X$\^]6 ? ?`ba?cdZfeX$gihj]9\jX[.] ...
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