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Peptide substrate

Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library

Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library

... Ydj1 peptide substrate were added to the ...indicates peptide substrate in ...A peptide GLYEIS with no detectable binding affinity to Ydj1 was used as negative ...the peptide ...

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Human cytomegalovirus maturational proteinase: expression in Escherichia coli, purification, and enzymatic characterization by using peptide substrate mimics of natural cleavage sites.

Human cytomegalovirus maturational proteinase: expression in Escherichia coli, purification, and enzymatic characterization by using peptide substrate mimics of natural cleavage sites.

... Kinetic parameters for the hydrolysis of HCMV M-site and R-site substrates by HCMV proteinase, calculated from the initial velocities of the peptide cleavages Peptide substrate site KM p[r] ...

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Development of novel assays for botulinum type A and B neurotoxins based on their endopeptidase activities

Development of novel assays for botulinum type A and B neurotoxins based on their endopeptidase activities

... (60-94) peptide substrate is incubated with sample containing BoNT/B, and peptide cleavage is detected by using an antibody which is specific to the newly exposed N terminus of the peptide ...

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Systematic profiling of ATP response to acquired drug-resistant EGFR family kinase mutations

Systematic profiling of ATP response to acquired drug-resistant EGFR family kinase mutations

... a peptide substrate, in which the ATP phosphate moiety was just covalently bonded to the hydroxyl group of peptide tyrosine ...The peptide can be readily removed from the structure leaving an ...

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A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases

A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases

... H4 peptide substrate and S-adenosyl methionine ...both substrate and product ...H4K20 substrate and generate an exclusively di- methylated ...

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Real-Time Video Imaging of Protease Expression In Vivo

Real-Time Video Imaging of Protease Expression In Vivo

... known peptide substrate and is adaptable to a wide range of real-time imaging applications with high throughputs such as for in vivo drug screening, examinations of the therapeutic efficacy of drugs, and ...

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5366.pdf

5366.pdf

... kinase substrate in a cell ...degradation-resistant peptide substrate for PKB ...Fluorescent peptide fragments were identified by comparison of their migration times with those of synthesized ...

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Structure and catalytic mechanism of the human histone methyltransferase SET7/9

Structure and catalytic mechanism of the human histone methyltransferase SET7/9

... of a ternary complex of human SET7/9 with a histone peptide and cofactor reveals that the peptide substrate and cofactor bind on opposite surfaces of the enzyme. The target lysine accesses the active ...

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Inhibition of secreted phospholipase A2 by neuron survival and anti-inflammatory peptide CHEC-9

Inhibition of secreted phospholipase A2 by neuron survival and anti-inflammatory peptide CHEC-9

... unmodified substrate, a nat- ural result of blocking of the enzyme binding to substrate by the ...accumulated substrate will eventu- ally compete successfully for the enzyme overcoming the ...

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Virus-Specific Cofactor Requirement and Chimeric Hepatitis C Virus/GB Virus B Nonstructural Protein 3

Virus-Specific Cofactor Requirement and Chimeric Hepatitis C Virus/GB Virus B Nonstructural Protein 3

... single-stranded peptide tether ...the substrate recognition site is occupied by the molecule’s own carboxy terminus, which provides a snapshot of the structure after cis proteolytic processing at the NS3/4A ...

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Twin arginine translocase (Tat) : structural and functional insight

Twin arginine translocase (Tat) : structural and functional insight

... the substrate, is ...containing substrate (S- DmsA RR _YFP) would dissociate less readily from the ...signal peptide laterally moves across the ...signal peptide to the TatBC complex where is ...

200

Inhibition of N1-Src kinase by a specific SH3 peptide ligand reveals a role for N1-Src in neurite elongation by L1-CAM

Inhibition of N1-Src kinase by a specific SH3 peptide ligand reveals a role for N1-Src in neurite elongation by L1-CAM

... rich peptide motifs 4–6 , which for C-Src comprise peptides in two orientations: Class I (+x PxxP; x is any amino acid, + is R or K, is hydrophobic) and Class II (PxxP x+) 7 ...enhance substrate ...

20

Evolutionarily Divergent Type II Protein Arginine Methyltransferase in Trypanosoma brucei

Evolutionarily Divergent Type II Protein Arginine Methyltransferase in Trypanosoma brucei

... TbPRMT5 displays intrinsic type II PRMT activity (Fig. 4 and 5) and does not appear to be dependent on additional cofactors for the modulation of this activity (Fig. 8). This re- port constitutes the first demonstration ...

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The Molecular Basis of Substrate Recognition by the Family of Pellino E3 Ubiquitin Ligases

The Molecular Basis of Substrate Recognition by the Family of Pellino E3 Ubiquitin Ligases

... Previous structural studies of the human Pellino2 substrate-recognition domain revealed an unexpected, non-canonical example of a forkhead-associated (FHA) domain (Figure 1.2) [30]. The FHA domain is a ...

103

In vitro biosynthetic studies of bottromycin expand the enzymatic capabilities of the YcaO superfamily

In vitro biosynthetic studies of bottromycin expand the enzymatic capabilities of the YcaO superfamily

... modified peptide (RiPP) family of natural ...the substrate tolerance for BmbD and BmbE and systematically dissect the role of the follower peptide, which we show serves a purpose similar to canonical ...

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Relationships Between Prior Experiences, Current Teaching Contexts, and Novice Teachers' Use of Concrete Representation for Mathematics Instruction

Relationships Between Prior Experiences, Current Teaching Contexts, and Novice Teachers' Use of Concrete Representation for Mathematics Instruction

... a peptide corresponding to residues 73-92 (hereinafter referred to as BMP-2 peptide) showed highest alkaline phosphatase (ALP) and osteogenic differentiation of MSCs, but the peptide activity was ...

238

The cyanobactin heterocyclase enzyme : A processive adenylase that operates with a defined order of reaction

The cyanobactin heterocyclase enzyme : A processive adenylase that operates with a defined order of reaction

... unreacted substrate, indicating that the rate of the second heterocyclization is significantly slower in these mutants but the rate of the first heterocyclization is largely ...core peptide), although very ...

7

Purification and Characterization of a Human Neutrophil Neutral Protease: THE NEUTRAL PEPTIDE GENERATING PROTEASE

Purification and Characterization of a Human Neutrophil Neutral Protease: THE NEUTRAL PEPTIDE GENERATING PROTEASE

... polyacrylamide gel electrophoresis of reduced and unreduced protein gave a single protein band of 29,000-30,000 mol wt. The isoelectric point determined in sucrose gradients ranged from pH 7.8 to 8.3 with a peak at pH ...

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Broadband Substrate to Substrate Interconnection

Broadband Substrate to Substrate Interconnection

... The top and side views of the proposed interconnection are shown in Figures 2(a) and (b), respectively. The heights of Substrate 1 and Substrate 2 are 0.5 mm and 0.2 mm, respectively. And its equivalent ...

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Vasonatrin peptide: a unique synthetic natriuretic and vasorelaxing peptide

Vasonatrin peptide: a unique synthetic natriuretic and vasorelaxing peptide

... The structures of human atrial natriuretic peptide h-ANP, human C-type natriuretic peptide h-CNP, and vasonatrin peptide VNP... The Biology of Vasonatrin Peptide..[r] ...

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