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Peptide substrate (E-D-A-E-Y-A-A-R-R-R-G)

Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library

Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library

... The molecular chaperones cover a large group of proteins that can recognize, bind and stabilize non-native polypeptides and facilitate protein folding (1-3). Molecular chaperone Heat shock protein 40s (Hsp40s) play ...

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Human cytomegalovirus maturational proteinase: expression in Escherichia coli, purification, and enzymatic characterization by using peptide substrate mimics of natural cleavage sites.

Human cytomegalovirus maturational proteinase: expression in Escherichia coli, purification, and enzymatic characterization by using peptide substrate mimics of natural cleavage sites.

... Kinetic parameters for the hydrolysis of HCMV M-site and R-site substrates by HCMV proteinase, calculated from the initial velocities of the peptide cleavages Peptide substrate site KM p[r] ...

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Development of novel assays for botulinum type A and B neurotoxins based on their endopeptidase activities

Development of novel assays for botulinum type A and B neurotoxins based on their endopeptidase activities

... (60-94) peptide substrate is incubated with sample containing BoNT/B, and peptide cleavage is detected by using an antibody which is specific to the newly exposed N terminus of the peptide ...

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A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases

A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases

... H4 peptide substrate and S-adenosyl methionine ...both substrate and product ...H4K20 substrate and generate an exclusively di- methylated ...

11

Real-Time Video Imaging of Protease Expression In Vivo

Real-Time Video Imaging of Protease Expression In Vivo

... known peptide substrate and is adaptable to a wide range of real-time imaging applications with high throughputs such as for in vivo drug screening, examinations of the therapeutic efficacy of drugs, and ...

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Systematic profiling of ATP response to acquired drug-resistant EGFR family kinase mutations

Systematic profiling of ATP response to acquired drug-resistant EGFR family kinase mutations

... a peptide substrate, in which the ATP phosphate moiety was just covalently bonded to the hydroxyl group of peptide tyrosine ...The peptide can be readily removed from the structure leaving an ...

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Structure and catalytic mechanism of the human histone methyltransferase SET7/9

Structure and catalytic mechanism of the human histone methyltransferase SET7/9

... the peptide-binding site, the channel surface is largely made up by the side chains of Leu 267 and Tyr residues 305, 335 and 337 ...for substrate or cofactor ...(histone peptide substrate) ...

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5366.pdf

5366.pdf

... kinase substrate in a cell ...degradation-resistant peptide substrate for PKB ...Fluorescent peptide fragments were identified by comparison of their migration times with those of synthesized ...

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Virus-Specific Cofactor Requirement and Chimeric Hepatitis C Virus/GB Virus B Nonstructural Protein 3

Virus-Specific Cofactor Requirement and Chimeric Hepatitis C Virus/GB Virus B Nonstructural Protein 3

... GBV-B NS3 protease processes its own polyprotein at the predicted sites. Several plasmids encoding the predicted GBV-B substrate cleavage sites at NS4A/4B, NS4B/5A, and NS5A/5B were constructed (Fig. 1) in order ...

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Inhibition of secreted phospholipase A2 by neuron survival and anti-inflammatory peptide CHEC-9

Inhibition of secreted phospholipase A2 by neuron survival and anti-inflammatory peptide CHEC-9

... [19,26,27]. The activity of the PLA2 enzymes has been associated with cell degeneration in many systems includ- ing the nervous system [28,29]. Furthermore, microglia and macrophages may depend on PLA2 activity for cell ...

9

Evolutionarily Divergent Type II Protein Arginine Methyltransferase in Trypanosoma brucei

Evolutionarily Divergent Type II Protein Arginine Methyltransferase in Trypanosoma brucei

... TbPRMT5 displays intrinsic type II PRMT activity (Fig. 4 and 5) and does not appear to be dependent on additional cofactors for the modulation of this activity (Fig. 8). This re- port constitutes the first demonstration ...

17

In vitro biosynthetic studies of bottromycin expand the enzymatic capabilities of the YcaO superfamily

In vitro biosynthetic studies of bottromycin expand the enzymatic capabilities of the YcaO superfamily

... modified peptide (RiPP) family of natural ...the substrate tolerance for BmbD and BmbE and systematically dissect the role of the follower peptide, which we show serves a purpose similar to canonical ...

6

Structure Based Design of Peptidomimetic Inhibitors of the MLL1-WDR5 Interaction.

Structure Based Design of Peptidomimetic Inhibitors of the MLL1-WDR5 Interaction.

... = 13 nM. However, 25-50 µM of MM-102 is required to inhibit HoxA9 expression and no inhibition was observed with MM-101 at the same concentrations. These observations suggest that those designed MLL1 mimetics may suffer ...

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Twin arginine translocase (Tat) : structural and functional insight

Twin arginine translocase (Tat) : structural and functional insight

... The purification of TatAdCd_S shows the highest yield is obtained when the cells are induced in the later stage of the growth cycle, such that the cells reach the stationary phase following 3hrs induction. Therefore as ...

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The Molecular Basis of Substrate Recognition by the Family of Pellino E3 Ubiquitin Ligases

The Molecular Basis of Substrate Recognition by the Family of Pellino E3 Ubiquitin Ligases

... Various structural studies of FHA domains in complex with their cognate phosphopeptide ligands have elucidated how FHA domains specifically recognize their ligands. For example, the X-ray crystal structure of ...

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Inhibition of N1-Src kinase by a specific SH3 peptide ligand reveals a role for N1-Src in neurite elongation by L1-CAM

Inhibition of N1-Src kinase by a specific SH3 peptide ligand reveals a role for N1-Src in neurite elongation by L1-CAM

... N1-Src functions in neurite elongation. We found that both CFP-PDN1 expression or shRNA knockdown of N1-Src reduces neurite elongation in cultured neurons. We have also observed that overexpression of N1-Src has a ...

20

Purification and Characterization of a Human Neutrophil Neutral Protease: THE NEUTRAL PEPTIDE GENERATING PROTEASE

Purification and Characterization of a Human Neutrophil Neutral Protease: THE NEUTRAL PEPTIDE GENERATING PROTEASE

... polyacrylamide gel electrophoresis of reduced and unreduced protein gave a single protein band of 29,000-30,000 mol wt. The isoelectric point determined in sucrose gradients ranged from pH 7.8 to 8.3 with a peak at pH ...

9

Capture and biological release of circulating tumor cells in pancreatic cancer based on peptide-functionalized silicon nanowire substrate

Capture and biological release of circulating tumor cells in pancreatic cancer based on peptide-functionalized silicon nanowire substrate

... With above design, as shown in Figure 1, Pe-SiNWS was established, in which staggered herringbone structure was adopted for the fabrication of microfluidic device, and nanowires were integrated onto channel ...

10

The cyanobactin heterocyclase enzyme : A processive adenylase that operates with a defined order of reaction

The cyanobactin heterocyclase enzyme : A processive adenylase that operates with a defined order of reaction

... unreacted substrate, indicating that the rate of the second heterocyclization is significantly slower in these mutants but the rate of the first heterocyclization is largely ...core peptide), although very ...

7

Relationships Between Prior Experiences, Current Teaching Contexts, and Novice Teachers' Use of Concrete Representation for Mathematics Instruction

Relationships Between Prior Experiences, Current Teaching Contexts, and Novice Teachers' Use of Concrete Representation for Mathematics Instruction

... a peptide corresponding to residues 73-92 (hereinafter referred to as BMP-2 peptide) showed highest alkaline phosphatase (ALP) and osteogenic differentiation of MSCs, but the peptide activity was ...

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