PFGE, pulsed-field gel electrophoresis
Original Article Application of pulsed-field gel electrophoresis (PFGE) in Bacillus cereus typing
... study, PFGE was used for typing ...The PFGE typing and homology analysis of 52 ...by pulsed-field gel ...different PFGE types, no dominant PFGE type was ...same ...
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DNA Fingerprinting of Methicillin Resistant Staphylococcus Aureus (MRSA) by Pulsed Field Gel Electrophoresis (PFGE) in a Teaching Hospital in Malaysia
... DNA Fingerprinting of Methicillin Resistant Staphylococcus Aureus (MRSA) by Pulsed Field Gel Electrophoresis (PFGE) in a Teaching Hospital in Malaysia ORIGINAL ARTICLE DNA Fingerprinting of Methicilli[.] ...
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Antibiotic resistance, virulence factors and genotyping of Uropathogenic Escherichia coli strains
... For identification of bacterial infection sources, it is in- fluential to establish relationships between different isolates of bacteria. It helps to determine the contamination sources, gaining insights into the ...
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Good Performance of the SpectraCellRA System for Typing of Methicillin Resistant Staphylococcus aureus Isolates
... standard. Pulsed-field gel electrophoresis (PFGE) was used for discrepancy ...for PFGE was ...for PFGE was 93.5% (49/53 households analyzed). With PFGE regarded as ...
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Comparative Evaluation of an Automated Repetitive Sequence Based PCR Instrument versus Pulsed Field Gel Electrophoresis in the Setting of a Serratia marcescens Nosocomial Infection Outbreak
... the PFGE and automated rep-PCR typing ...the PFGE and rep-PCR patterns of each ...the PFGE profiles, 1 major pattern (type A) and 12 unique genetically unrelated patterns (types B through O) were ...
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Use of pulsed field gel electrophoresis for epidemiological study of Bordetella pertussis in a whooping cough outbreak
... We used pulsed-field gel electrophoresis PFGE of chromosomal DNA digested with XbaI to determine the distribution of different BordeteUa pertussis strains from clinical isolates obtained[r] ...
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Analysis of Genetic Diversity of Streptococcus suis Clinical Isolates from Pigs in Spain by Pulsed Field Gel Electrophoresis
... by PFGE within the most frequently isolated capsular types in this study (9, 2, and 7) with 24, 10, and 6 different pulsotypes, ...Thus, PFGE was proven to be a more discrimi- natory and accurate technique ...
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Characterization of Isolates of Methicillin Resistant Staphylococcus aureus from Hong Kong by Phage Typing, Pulsed Field Gel Electrophoresis, and Fluorescent Amplified Fragment Length Polymorphism Analysis
... interpret PFGE results, and strains need to be compared to epidemic strains where ...on PFGE and antibiogram by using a combination of methods including phage typing and fluorescent amplified frag- ment ...
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Molecular Typing of Selected Enterococcus faecalis Isolates: Pilot Study Using Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis
... Evaluation of minimum number of loci needed for subtyp- ing. We also examined the possibility that the sequence vari- ation of fewer loci might be adequate for subspecies typing. The allelic profiles from two antigenic ...
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Computer Assisted Analysis and Epidemiological Value of Genotyping Methods for Campylobacter jejuni andCampylobacter coli
... typing), pulsed-field gel electrophoresis (PFGE), automated ribotyping, and amplified fragment length polymorphism (AFLP) fingerprinting were ...while PFGE identified 38 ...
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Typing and Characterization of Mechanisms of Resistance ofShigella spp Isolated from Feces of Children under 5 Years of Age from Ifakara, Tanzania
... tools, pulsed-field gel electrophoresis (PFGE), repetitive extragenic palindromic (REP)-PCR, plasmid analysis, and antibiogram, were compared for typing Shigella ...either PFGE ...
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Genomic Comparisons and Shiga Toxin Production among Escherichia coli O157:H7 Isolates from a Day Care Center Outbreak and Sporadic Cases in Southeastern Wisconsin
... electric field pulsed-field gel electrophoresis (CHEF-PFGE) was used to compare Wisconsin isolates of Escherichia coli O157:H7, including 39 isolates from a 1994 day care center ...
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Increase in local protein concentration by field-inversion gel electrophoresis
... Pulsed-field gel electrophoresis (PFGE) is an elegantly simple and universally accepted technique for the separa- tion of large DNA molecules ...of PFGE with different ...
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Evaluation of Pulsed Field Gel Electrophoresis as a Tool for Determining the Degree of Genetic Relatedness between Strains of Escherichia coli O157:H7
... for PFGE of O157 was used, with some modifi- cations ...temperatures). PFGE was performed on a CHEF-DRII PFGE apparatus (Bio-Rad) using the following parameters: separation on a 1% agarose gel ...
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Review on Molecular Typing Methods of Pathogens
... methods, Pulsed-Field Gel Electrophoresis (PFGE), Restriction Fragment Length Polymorphisma (RFLP), Random Amplified Polymorphic DNA (RAPD), Automatic Ribotyping, rep-PCR, Multi-Locus ...
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Clonal Composition of Staphylococcus aureus Isolates at a Brazilian University Hospital: Identification of International Circulating Lineages
... using pulsed-field gel electrophoresis (PFGE), multilocus restriction fragment typing (MLRFT), MLST, and PCR-based genotyping for the presence of the four allotypes of SCCmec and ...
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Comparison between Salmonella enterica Serotype Enteritidis Genotyping Methods and Phage Type
... (MLVA), pulsed-field gel electrophoresis (PFGE), and automated ribotyping, with a new combination of restriction enzymes, to a collection of 238 ...capillary electrophoresis ...
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Use of Highly Discriminatory Fingerprinting to Analyze Clusters of Clostridium difficile Infection Cases Due to Epidemic Ribotype 027 Strains
... American pulsed-field type 1 [NAP1], or group BI by restriction endo- nuclease analysis [REA]) and 001; these strains were referred to as pulsovars A and B, respectively, as determined by pulsed- ...
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Multilocus Sequence Typing Compared to Pulsed Field Gel Electrophoresis for Molecular Typing of Pseudomonas aeruginosa
... PFGE. PFGE was performed as described previously ...for gel analysis with Fingerprinting II software (Bio-Rad ...the pulsed-field type clusters ...
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A combinational approach of multilocus sequence typing and other molecular typing methods in unravelling the epidemiology of Erysipelothrix rhusiopathiae strains from poultry and mammals
... on pulsed-field gel electrophoresis (PFGE) and MLST, (iii) determine the diversity of the dominant immunogenic protein SpaA, and (iv) examine the distribution of genes putatively linked ...
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