pHi, intracellular pH
The Effect of Curcumin on Intracellular pH (pHi), Membrane Hyperpolarization and Sperm Motility
... The pHi plays an important role in modulating mammalian sperm motility (12, 17, ...acidic pHi inhibits sperm motility, which is in agreement with our ...The pHi of ...the pHi of control mu- ...
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Effects of bafilomycin A1 on cytosolic pH of sheep alveolar and peritoneal macrophages: evaluation of the pH regulatory role of plasma membrane V ATPases
... and, in cells such as osteoclasts and renal tubular cells, serve to acidify specialized extracellular compartments (Chatterjee et al. 1992; Gluck and Nelson, 1992). In macrophages (m f ), plasma membrane V-ATPases are ...
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Regulation of intracellular pH in the rabbit cortical collecting tubule
... the pH sensitive dye 2',7'-bis- (2-carboxyethyl)-5(and-6)carboxyfluorescein (BCECF) and basolateral Cl- removal were used to identify and study intracellular pH (pHi) regulation in each of ...
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The influence of photosynthesis on host intracellular pH in scleractinian corals
... seawater pH was deliberately controlled in order to identify intracellularly driven pHi ...coral pHi with changes in extracellular pH in both the coelenteron lumen and the external seawater ...
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Is intracellular pH a clock for mitosis?
... As pHi increases beyond interphase the presence of nucleating centers, along with the favoring of microtubule polymerization in a higher pHi environment, suggests that the pool of tubulin from interphase ...
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Regulation of Intracellular pH by Toadfish (Opsanus Beta) Hepatocytes
... Buffering capacity, membrane potential and regulation of pHi under simulated physiological conditions Non-bicarbonate buffering capacity in toadfish hepatocytes was calculated by using t[r] ...
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THE RELATIONSHIP BETWEEN INTRACELLULAR pH AND SWIMMING PERFORMANCE OF BROWN TROUT EXPOSED TO NEUTRAL AND SUBLETHAL pH
... sublethal pH in the present experiments, then when sufficient power could not be provided by aerobic metabolism, at a relatively low swimming speed, ATP consumption would exceed its production, thus producing ...
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Beta adrenergic upregulation of the Na(+) K(+) 2Cl cotransporter in rat parotid acinar cells
... the pH-sensitive fluorescent dye 2',7'-bis(2-carboxyethyl)-5(6')-carboxyfluorescein to monitor the recovery of the intracellular pH (pHi) of rat parotid acini from an NH4(+)- induced alkaline ...
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Axial heterogeneity of intracellular pH in rat proximal convoluted tubule
... profile, intracellular pH (pHi) was measured along the superficial PT of the rat under free-flow and stationary microperfusion using the pH-sensitive fluorescence of 4- methylumbelliferone ...
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Intracellular pH modulates the generation of superoxide radicals by human neutrophils
... of intracellular pH (pHi) to superoxide radical (O2-) generation was investigated in chemotactic factor-stimulated human ...in pHi from 7.22 to 7.80. This pHi change was sensitive to ...
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Control and modulation of intracellular pH in mammalian central neurones
... different pH until a full calibration of ratio against pHj had been ...particular pHi values varied somewhat from cell to cell (see Chapter 3) so this necessitated calibration of individual experimental ...
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Treatment of Lactic Acidosis with Dichloroacetate in Dogs
... blood pH and levels of bicarbonate and lactate; the intracellular pH (pHi) in liver and skeletal muscle; cardiac index, arterial blood pressure and liver blood flow; liver lactate uptake and ...
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Effect of isoproterenol on intracellular pH of the intercalated cells in the rabbit cortical collecting ducts
... 6)carboxyfluorescein. The pHi of beta-IC was significantly decreased with the addition of basolateral 10(-6) M isoproterenol (7.21 +/- 0.04 to 7.05 +/- 0.04), whereas alpha-IC did not show any change. This ...
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Intracellular pH regulation and proton transport by rabbit renal medullary collecting duct cells Role of plasma membrane proton adenosine triphosphatase
... membranes, intracellular pH (pHi) regulation and proton extrusion rates were measured in freshly prepared suspensions of rabbit outer MCD ...centrifugation. pHi was estimated fluorometrically ...
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Na+/H+ exchange and osmotic shrinkage in isolated trout hepatocytes
... Fish hepatocytes have been little studied with respect to the regulation of their intracellular pH. In a series of studies on the rainbow trout Oncorhynchus mykiss (a freshwater species) and on the toadfish ...
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Interdependence of Ca2+ and proton movements in trout hepatocytes
... attenuated, the thapsigargin-induced alkalinization, while in the presence of ionomycin the alkalinization was almost abolished. This indicates that the response to thapsigargin is not completely due to Ca 2+ changes, ...
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Intracellular pH in the toad Bufo marinus following hypercapnia
... Non-bicarbonate buffer values determined by titrating tissue homogenates in vitro ft and apparent in vivo non-bicarbonate buffer values f3' calculated from normocapnic and hypercapnic 5 [r] ...
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Intracellular pH regulation in unstimulated Calliphora salivary glands is Na+ dependent and requires V ATPase activity
... A molecular approach was chosen to confirm the existence of an EAAT more strongly than by the pH measurements described above. We amplified a 194bp fragment of EAAT (Cv EAAT) from blowfly salivary gland cDNA. For ...
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CALCULATION OF INTRACELLULAR pH FROM THE DISTRIBUTION OF 5,5 DIMETHYL 2,4 OXAZOLIDINEDIONE (DMO) APPLICATION TO SKELETAL MUSCLE OF THE DOG
... Of even greater interest than the calculated CENTRATION IN EXTRACELLULAR TISSUE WATER CE pH for the normal cell are the changes that this value WITH RESPECT TO INTRACELLULAR PH pH, AS A [r] ...
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CD8 T cells undergo activation and programmed death-1 repression in the liver of aged Ae2
... were also anesthetized with isoflurane and the liver was perfused through the portal vein with PBS (Gibco, Life Technologies) for about 5 minutes to wash away circulating lymphocytes. Anesthetized mice were then ...
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