phorbol 12-myristate
Nanomechanical measurement of adhesion and migration of leukemia cells with phorbol 12-myristate 13-acetate treatment
... of phorbol 12-myristate 13-acetate (PMA) treatment, the conventional batch-cell transwell-migration assay may not indicate the intrinsic effect of the treatment on migration, since the treatment may ...
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Inhibition of Osteopontin Synthesis in THP-1 Cells Stimulated with Phorbol 12-Myristate 13-Acetate by Brefelamide Derivatives
... non- phorbol 12-myristate 13-acetate (PMA)-stimulated cells by ELISA, with increased levels of all forms, including undefined Ud-OPN, in PMA-stimulated cells ...
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Geraniol suppresses proinflammatory mediators in phorbol 12-myristate 13-acetate with A23187-induced HMC-1 cells
... Figure 4 geraniol mediates activation of nF-κB and MaPK signaling pathways. Notes: hMc-1 cells were pretreated with various concentrations of geraniol for 1 hour and stimulated with PMaci for 6 hours. The expression of ...
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Evaluation of effects of various drugs on platelet functions using phorbol 12-myristate 13-acetate-induced megakaryocytic human erythroid leukemia cells
... HEL cells anytime/anywhere without sampling blood. In the present study, attempts were made to improve the evaluation method for evaluating the effects of various drugs (aspirin and cilostazol, antiplatelet drugs; ...
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Protein kinase C activation mediates interferon-β-induced neuronal excitability changes in neocortical pyramidal neurons
... -phorbol 12-myristate 13-acetate or IFN- β in ex vivo whole-cell recordings of layer 5 neocortical neurons and in silico ...-phorbol 12-myristate 13-acetate (4 β -PMA) (1 μ M) ...
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Soluble CD40L is associated with increased oxidative burst and neutrophil extracellular trap release in Behçet’s disease
... Hydrogen peroxide and superoxide anion quantification Neutrophils and PBMCs were separated by density gra- dient separation using dextran and Ficoll-Hypaque 1077, respectively. Cells were stimulated with (1) 30 ng/ml ...
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Reduced glutathione as a physiological co activator in the activation of peptidylarginine deiminase
... Fig. 4 Release of catalytically active peptidylarginine deiminase ( PAD ) from PMA-stimulated granulocytes. a Microtitre plates coated with human fibrinogen were incubated for 3 h at RT with blood leucocytes resuspended ...
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Pharmacological targeting of <em>β</em>-adrenergic receptor functions abrogates NF-κB signaling and MMP-9 secretion in medulloblastoma cells
... receptors was confirmed in DAOY cells by semiquantitative RT-PCR. We next found that propranolol dose-dependently inhibited induction of the key extracellular matrix-degrading and blood–brain barrier disrupting enzyme ...
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Priming of polymorphonuclear neutrophils by atrial natriuretic peptide in vitro
... anion. Phorbol 12-myristate 13-acetate, opsonized zymosan, or FMLP could all be used as triggering stimuli to demonstrate the priming of PMN activation by ...
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Low production of reactive oxygen species in granulocytes is associated with organ damage in systemic lupus erythematosus
... Figure 1 Polymorphonuclear leukocytes (PMN) from patients with systemic lupus erythematosus (SLE) produced fewer reactive oxygen species (ROS) than PMN from healthy blood donors. The capacities of PMN from healthy blood ...
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Original Article PKCδ reveals a tumor promoter function by promoting cell proliferation and migration in somatotropinomas
... Abstract: Protein kinase C δ (PKCδ), a subtype of PKC family, has been recognized as a tumor promoter or suppres- sor depending on different tissue specificities in various tumor types. However, the effects of PKCδ on ...
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Effects of protein kinase C activation on sodium, potassium, chloride, and total CO2 transport in the rabbit cortical collecting tubule
... C, phorbol 12-myristate,13-acetate (PMA) and L-alpha-1,2-dioctanoylglycerol (L-alpha-1,2-DOG), and two inactive analogues, 4 alpha-phorbol and 4-O-methyl phorbol ...
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High-Mobility-Group Protein I Can Modulate Binding of Transcription Factors to the U5 Region of the Human Immunodeficiency Virus Type 1 Proviral Promoter
... sic differences in the stability of the HMG I and HMG Y proteins (although both proteins appear to be extremely stable in living cells) (6). A similar selective enhancement of HMG I protein by tetradecanoyl ...
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Non-typeable Haemophilus influenzae decreases cilia beating via protein kinase C epsilon
... ml phorbol myristate acetate (PMA), 30 mM dithiothrei- tol, 150 μM ATP, 45 mM magnesium acetate, and 10 μCi/ml [γ- 32 P] ATP in a Tris–HCl buffer (pH ...
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Vγ9Vδ2 T Cells in Human Legionellosis
... Legionella is a genus of gram-negative bacteria and the cause of two different clinical entities. One of them is Legionnaires’ disease, a severe pneumonic disease associated with a rela- tively low attack rate, a long ...
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Niacinamide mitigated the acute lung injury induced by phorbol myristate acetate in isolated rat's lungs
... effectively abrogated the lung injury caused by phorbol myristate acetate in isolated lungs. Niacinamide dimin- ished the pulmonary hypertension and increase in microvascular permeability following PMA ...
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Effects of Phorbol Myristate Acetate on the Metabolism and Ultrastructure of Neutrophils in Chronic Granulomatous Disease
... that phorbol myristate acetate (PMA), the active principle of croton oil, stimulates alterations in normal polymorphonuclear leukocytes (PMN) that resemble closely the changes that develop in the cells ...
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Inhibition of vasopressin stimulated water flow in toad bladder by phorbol myristate acetate, dioctanoylglycerol, and RHC 80267 Evidence for modulation of action of vasopressin by protein kinase C
... The action of vasopressin (AVP) in transporting epithelia is mediated by cyclic AMP(cAMP), whereas its effects in hepatocytes are mediated by calcium and phosphoinositides. Based on our recent observation that AVP ...
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NAD(P)H oxidase activity in human neutrophils stimulated by phorbol myristate acetate
... seen. Phorbol myristate acetate-induced O27 production was noted to be modulated by pH in intact neutrophils, suggesting that NAD(P)H oxidase is localized in the plasma membrane where its activity may be ...
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Induction of chronic human immunodeficiency virus infection is blocked in vitro by a methylphosphonate oligodeoxynucleoside targeted to a U3 enhancer element.
... One such oligomer, targeted to an NF-KB enhancer element, inhibited phorbol myristate acetate induction of viral replication and tat-mediated trans activation of the human immunodeficien[r] ...
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