phosphatidylinositol 4
A Single Point Mutation in the Rhinovirus 2B Protein Reduces the Requirement for Phosphatidylinositol 4-Kinase Class III Beta in Viral Replication
... The formation of the replication membranes also critically depends on host factors such as phosphatidylinositol 4 (PI4)-kinase class 3b (PI4K3b) (21, 22, 27). In uninfected cells, PI4K3b is located at the ...
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Evaluation of Phosphatidylinositol-4-Kinase IIIα as a Hepatitis C Virus Drug Target
... is phosphatidylinositol-4- kinase III ␣ (PI4KIII ␣ ) (10, 15, 43, 50, 59, 63, ...mammalian phosphatidylinositol 4-kinases that catalyze the first step in phosphoinositide synthesis ...
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skittles, a Drosophila Phosphatidylinositol 4-Phosphate 5-Kinase, Is Required for Cell Viability, Germline Development and Bristle Morphology, But Not for Neurotransmitter Release
... The phosphatidylinositol pathway is implicated in the regulation of numerous cellular functions and responses to extracellular ...of phosphatidylinositol 4-phosphate by the ...
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Phosphatidylinositol 4-Kinase III-β Is Required for Golgi Maintenance and Cytokinesis in Trypanosoma brucei
... III phosphatidylinositol 4-kinases ( ␣ and  ...III phosphatidylinositol 4-kinase  (TbPI4KIII-  ), expressed the protein in COS-7 cells, and confirmed that the protein catalyzes the ...
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Enterovirus 3A Facilitates Viral Replication by Promoting Phosphatidylinositol 4-Kinase IIIβ–ACBD3 Interaction
... ABSTRACT Like other enteroviruses, enterovirus 71 (EV71) relies on phosphatidy- linositol 4-kinase III  (PI4KB) for genome RNA replication. However, how PI4KB is re- cruited to the genome replication sites of ...
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Phosphatidylinositol 4-Kinase III Beta Is a Target of Enviroxime-Like Compounds for Antipoliovirus Activity
... a phosphatidylinositol 4-kinase III beta (PI4KB) inhibitor, PIK93, was reported, suggesting that PI4KB is an important host factor targetable by antienterovirus compounds ...
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Hepatitis C Virus NS5A Hijacks ARFGAP1 To Maintain a Phosphatidylinositol 4-Phosphate-Enriched Microenvironment
... Phosphatidylinositol 4-phosphate (PI4P) is well known to be upregulated during hepatitis C virus (HCV) replication. The role of PI4 kinases in HCV has been extensively investigated. Whether the PI4P ...
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A Complex Comprising Phosphatidylinositol 4-Kinase IIIβ, ACBD3, and Aichi Virus Proteins Enhances Phosphatidylinositol 4-Phosphate Synthesis and Is Critical for Formation of the Viral Replication Complex
... Phosphatidylinositol 4-kinase III  (PI4KB) is a host factor required for the replication of certain picornavirus ...product phosphatidylinositol 4-phosphate (PI4P) colocalize to the AiV RNA ...
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Hepatitis C Virus Stimulates the Phosphatidylinositol 4-Kinase III Alpha-Dependent Phosphatidylinositol 4-Phosphate Production That Is Essential for Its Replication
... Phosphatidylinositol 4-kinase III alpha (PI4KA) is an essential cofactor of hepatitis C virus (HCV) repli- ...in phosphatidylinositol 4-phosphate (PI4P) accumulation throughout the cytoplasm, ...
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Modulation of phosphatidylinositol 4-phosphate levels by CaBP7 controls cytokinesis in mammalian cells
... Figure S1 – Dot blot analysis of cellular PI4P levels in cells over-expressing CaBP7. Total cellular lipid was extracted from HeLa cells overexpressing mCh-CaBP7 or mCh- N1 control plasmid and spotted in the centre of a ...
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Type II phosphatidylinositol 4 kinase regulates trafficking of secretory granule proteins in Drosophila
... Type II phosphatidylinositol 4-kinase (PI4KII) produces the lipid phosphatidylinositol 4-phosphate (PI4P), a key regulator of membrane trafficking. Here, we generated genetic models of the ...
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Mapping of Functional Domains of the Lipid Kinase Phosphatidylinositol 4-Kinase Type III Alpha Involved in Enzymatic Activity and Hepatitis C Virus Replication
... FIG 5 Mapping of the N-terminal border required for PI4KIII ␣ function. (A) Huh7-Lunet T7 cells were stably transduced with selectable lentiviral vectors expressing nontargeting shRNA (NT) or shRNA targeting endogenous ...
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Biochemical and Genetic Evidence for the Presence of Multiple Phosphatidylinositol- and Phosphatidylinositol 4,5-Bisphosphate-Specific Phospholipases C in Tetrahymena
... sengers by initiating Ca 2⫹ mobilization and protein phosphor- ylation, respectively; the activation of this signaling pathway dictates cellular fate by regulating functions such as cellular activation, proliferation, ...
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Class III Phosphatidylinositol 4-Kinase Alpha and Beta Are Novel Host Factor Regulators of Hepatitis C Virus Replication
... ⫺ 4 standard deviations below the median ...⫺ 4, Table 1), although cy- clophilin F siRNAs scored below ⫺ 2 standard deviations (see Table S1 in the supplemental ...⫺ 4 standard deviations from the ...
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Soluble uric acid increases PDZK1 and ABCG2 expression in human intestinal cell lines via the TLR4 NLRP3 inflammasome and PI3K/Akt signaling pathway
... The aim of this article is to explore the relationship between soluble uric acid and gut excretion as well as the relevant mechanisms. In this study, HT-29 and Caco-2 cells were used as well-established models of human ...
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Phosphatidylinositol-5-phosphate 4-kinase gamma accumulates at the spindle pole and prevents microtubule depolymerization
... tion, phosphatidylinositol 4-phosphate 5-kinase alpha (PIP5KIα) has recently been demonstrated to regulate neuronal MT depolymerase kinesin KIF2A and suppress the elongation of axon branches ...two ...
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Diets involved in PPAR and PI3K/AKT/PTEN pathway may contribute toneuroprotection in a traumatic brain injury
... the major second messenger of the PI3K pathway that mediates receptor tyrosine kinase signaling to the sur- vival kinase AKT. PTEN negatively regulates the activity of PI3K/AKT signaling by converting PIP3 to PIP2 ...
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Arenavirus Infection Induces Discrete Cytosolic Structures for RNA Replication
... lipid phosphatidylinositol-4-phosphate and proteins involved in cellular mRNA metabolism, including the large and small ribosomal subunit proteins L10a and S6, the stress granule protein G3BP1, and a subset ...
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Biochemical and functional characterisation of phospholipase C η2
... the enzymatic cleavage of phosphatidylinositol (PI) and phosphatidylinositol 4- monophosphate (PI(4)P) but it never cleaves 3-phosphoinositides. Moreover, Ca 2+ is essential to be present at ...
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PIP5K dependent production of PIP2 sustains microtubule organization to establish polarized transport in the Drosophila oocyte
... Using the Drosophila oocyte as a model system, we show that the type I phosphatidylinositol 4-phosphate 5-kinase PIP5K, Skittles, is necessary to sustain the organization of microtubules[r] ...
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