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Plant Tissue Culture & Genetic Transformation

Assessment of plant transformation technology of maize

Assessment of plant transformation technology of maize

... problem, genetic engineering was ...increasing genetic resource to improve crop and also introduce known function of genes to achieve the goals of crop improvement by genetic engineering programme ...

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Transgenic Plant Production using Different T-DNA Technologies as a Tool of Genetic Engineering

Transgenic Plant Production using Different T-DNA Technologies as a Tool of Genetic Engineering

... The transformation procedure was carried out as described by McCormick et ...bacterial culture suspension with gentle agitation, blotted to sterilized Whatman ...B. Tissue culture-derived ...

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Recent Progress of Transgenic Technology Development for Alfalfa

Recent Progress of Transgenic Technology Development for Alfalfa

... subsequently plant recovery [29] [40] ...concerning transformation is that the callus can be maintained for proliferation for an extended period of time and thus the selection for transgenic calli can be ...

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FluxTransgenics: a flexible LIMS-based tool for management of plant transformation experimental data

FluxTransgenics: a flexible LIMS-based tool for management of plant transformation experimental data

... the transformation process per- formed at the Cellular Biology and Plant Tissue Culture Laboratory at Embrapa Maize and Sorghum using ...the transformation process, including the clones ...

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Tissue Culture-based Agrobacterium -mediated and in planta Transformation Methods

Tissue Culture-based Agrobacterium -mediated and in planta Transformation Methods

... the plant nuclear genome (Gelvin 2000; Tzfira & Citovsky ...of genetic transformation of plants (Zupan et ...the plant genome (Meyers et ...into plant genome (Gelvin 2000; Tzfira ...

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Investigation of Cell-Penetrating Peptide Transformation in Two Regenerable Tissue Culture Systems

Investigation of Cell-Penetrating Peptide Transformation in Two Regenerable Tissue Culture Systems

... CPP-mediated transformation has garnered a lot of interest in genetic transformation, especially concerning its application in ...novel plant transformation method, which may in turn ...

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The roles of bacterial and host plant factors in Agrobacterium mediated genetic transformation

The roles of bacterial and host plant factors in Agrobacterium mediated genetic transformation

... On the bacterial side, numerous genes have been proposed to play a role in the initial attachment; however, for most of them, a function could not be defined, and many of them were found to be not essential for ...

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Advances in Somatic Embryogenesis Research of Horticultural Plants

Advances in Somatic Embryogenesis Research of Horticultural Plants

... The genotype is the key factor in affecting plant SE. The frequency of SE has been shown to be quite diverse be- cause of genotypic variation, even within the same genus. Not all of the species of an induced genus ...

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Glucosinolates content of in vitro grown Nasturtium officinale (watercress)

Glucosinolates content of in vitro grown Nasturtium officinale (watercress)

... wild plant of ...vitro culture. All plant cultures were kept inside growth chamber at 25°C under 16 hours photoperiod for thirty days before sub-cultured into fresh medium containing ...non-treated ...

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Shoot Induction in White Eggplant (Solanum melongena L. Cv. Bulat Putih) using 6-Benzylaminopurine and Kinetin

Shoot Induction in White Eggplant (Solanum melongena L. Cv. Bulat Putih) using 6-Benzylaminopurine and Kinetin

... diet. Plant transformation studies on the eggplant have been widely done for the production of transgenic eggplants harbouring genes that are beneficial for optimal plant growth and fruit ...

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Study of the Comparative Performance of Citrus Aurantifolia Plants Grown on Their Own Roots and Raised by Micro-Grafting of Nucellar Plantlets

Study of the Comparative Performance of Citrus Aurantifolia Plants Grown on Their Own Roots and Raised by Micro-Grafting of Nucellar Plantlets

... Like, excised shoot meristem of Citrus, the success has been eluding to clone it by employing nodal stem-segments, excised from mature trees (Groser, 1994).Recently, success has been achieved in cloning mature elite ...

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Tissue Culture Study of the Medicinal Plant Leek (Allium ampeloprasum L.)

Tissue Culture Study of the Medicinal Plant Leek (Allium ampeloprasum L.)

... of plant tissue culture was proposed in 1902 by Gottlieb Haberlandt of The German Academy of ...base culture medium containing different amounts of benzyl aminopurine- 6 (BAP) or naphtyl ...

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Regeneration of in Vitro Shoot and Root Structure through Hormone Manipulation of Coconut (MATAG F2) Zygotic Embryos

Regeneration of in Vitro Shoot and Root Structure through Hormone Manipulation of Coconut (MATAG F2) Zygotic Embryos

... 2.1. Plant Material, Aseptic Culture and Browning Secretion The experiments of this study were carried out at Tissue Culture Research La- boratory, Biotechnology and Nanotechnology Research ...

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A critical appraisal on plant tissue culture studies in medicinally important plants

A critical appraisal on plant tissue culture studies in medicinally important plants

... of plant tissue culture methods has gained thrust to meet the growing demands for pharmaceutical industries since the genetic diversity of medicinal plants is decreasing hap ...time. ...

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Kinetic and cytological analyses of plant tissue cultures : an approach to genetic manipulation of plant somatic cells

Kinetic and cytological analyses of plant tissue cultures : an approach to genetic manipulation of plant somatic cells

... 2 CELL CYCLE ANALYSIS OF THE CAPT SUSPENSION CELL CULTURE Estimation of Growth Paramete rs Labelling S - Phase Analysis Mitotic and Labelling Indices Cell Cycle Analyses Growth Curves La[r] ...

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In vitro evaluation of plant growth regulators on tissue culture bioassay produced by Pseudomonas species

In vitro evaluation of plant growth regulators on tissue culture bioassay produced by Pseudomonas species

... Auxins, gibberellins and cytokinins produced extra cellularly in nutrient broth by Pseudomonas sp. at best optimum conditions i.e. 28° C under shake conditions (90 rpm) after 72 hours. Auxins and gibberellins were ...

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Turmeric powder ( Curcuma longa Linn. ) as an antifungal agent in plant tissue culture studies

Turmeric powder ( Curcuma longa Linn. ) as an antifungal agent in plant tissue culture studies

... in-vitro plant culture has been strictly based on the use of sterile ...the plant tissue must typically be carried out in a filtered-air environment, ...threatens plant tissue ...

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PROPAGATION AND CHEMICAL MUTGENIC INDUCTION OF EUSTOMA GRANDIFLORUM PLANT USING TISSUE CULTURE TECHNIQUE

PROPAGATION AND CHEMICAL MUTGENIC INDUCTION OF EUSTOMA GRANDIFLORUM PLANT USING TISSUE CULTURE TECHNIQUE

... Mutagenesis is one of the powerful tools for breeding biotechnology. Chemical mutagenesis is an easy tool, which doesn’t require special tools and provide high mutation rate. Effects of different cytokines (BA, 2ip and ...

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EFFECT OF GROWTH REGULATORS ON IN VITRO CULTURES OF TWO BASMATI RICE GENOTYPES: RANBIR BASMATI AND BASMATI 370

EFFECT OF GROWTH REGULATORS ON IN VITRO CULTURES OF TWO BASMATI RICE GENOTYPES: RANBIR BASMATI AND BASMATI 370

... different concentrations of 2, 4-D alone were evaluated for callus induction from dehusked seed of Ranbir basmati and Basmati 370. The callus initiation started 15 days after inoculation of dehusked seeds of both Ranbir ...

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Plant regeneration: cellular origins and molecular mechanisms

Plant regeneration: cellular origins and molecular mechanisms

... Culturing plant explants on auxin-rich callus-inducing medium upregulates the expression of PLT3, PLT5 and PLT7, which subsequently promotes the acquisition of pluripotency through the induction of PLT1, PLT2, ...

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