Plasmid, switch construction, and cell strains
Generation of Mutant Murine Cytomegalovirus Strains from Overlapping Cosmid and Plasmid Clones
... the construction of mutant MCMVs in- volved the introduction of viral DNA and a plasmid bearing the target gene plus an extraneous selection and/or reporter gene into permissive ...and plasmid system ...
8
Construction and Characterization of a Replication-Competent Retroviral Shuttle Vector Plasmid
... The retroviral life cycle depends on the conversion of the RNA genome found in virions into DNA and the subsequent integration of the DNA into the host cell genome. There are a number of different types of ...
7
Construction of Mtb72F Plasmid as a DNA Vaccine Candidate for Mycobacterium tuberculosis
... Mtb72F consists of the C- and N-terminal open reading frames (ORFs) of Mtb32 (Rv0125) with the mtb39 gene (Rv1196) between them. The resulting recombinant vaccine, Mtb72F, provided greater protection against TB in ...
7
Characterization of Shigella Strains in Iran by Plasmid Profile Analysis and PCR Amplification of ipa Genes
... during cell storage and subculturing or plasmid ...of plasmid DNA revealed that all 82 isolates harbored multiple plasmids, with an average of ...all strains and a mean of 10, 8, 10, and 9 ...
5
For the construction of the Egr1 expression plasmid, the pcmv-sport6-egr1 vector (Invitrogen),
... Deparaffinization, endogenous peroxidase inactivation, antigen retrieval from formalin fixed paraffin embedded clinical tissues, and immunostaining of Brm was performed as described previously (14). For Egr1 ...
13
SWITCH: a dynamic CRISPR tool for genome engineering and metabolic pathway control for cell factory construction in Saccharomyces cerevisiae
... that SWITCH can be used to integrate and fine tune a metabolic pathway to increase produc- tion ...a plasmid as it allowed us to rapidly identify functional ...if SWITCH mediated pathway fine tuning ...
12
Construction of Escherichia coli Strains for Conversion of Nitroacetophenones to ortho-aminophenols
... constructed strains might accumulate the aminophenols from nitroacetophenones and other nitroaromatic ...recombinant plasmid carrying NB nitroreductase (nbzA) and HAB mutase A (habA) genes, both from ...
8
Construction of Bacillus thuringiensis simulant strains suitable for environmental release
... insertion plasmid and grown on LB agar plates containing spectinomycin at ...expression plasmid pAD02, and then grown on LB agar plates containing tetracycline at ...
16
Efficient construction of Streptococcus anginosus mutants in strains of clinical origin
... Molecular experiments to study gene functions usually require tools for DNA and cell manipulation. Those tools include methods to introduce and stably maintain DNA in bacterial cells and methods to analyze the ...
9
The construction of the eukaryotic expression plasmid pcDNA3.1/azurin and the increased apoptosis of U2OS cells transfected with it
... The infection of tumor-bearing mice with live attenuated cells of Salmonella typhimurium has been reported to induce tumor regression [1, 2]. A significant regression of subcutaneous tumors in mice was observed when ...
15
Construction of an Optimized Helper Plasmid Containing Vpx for Lentiviral-Mediated Transduction of Dendritic Cells
... transduction is associated with expression of specific protein SAMHD1 in dendritic cells, the phosphohydrolase that decomposes dNTP to inorganic phosphate and nucleoside, thereby reducing the pool of dNTPs and preventing ...
6
DEMONSTRATION OF TRANSMISSION OF ANTIBIOTIC RESISTANT PLASMID FROM RESISTANT TO SENSITIVE STRAINS OF ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE AT 370C
... A plasmid is a small DNA molecule that is physically separate from, and can replicate independently of, chromosomal DNA within a ...2010). Plasmid-mediated transfer of drug-resistance genes among bacterial ...
10
RISK SURVEILLANCE OF MULTIDRUG RESISTANT PSEUDOMONAS AERUGINOSA IN WATER AND PLASMID RELATEDNESS WITH CLINICAL STRAINS IN ABEOKUTA, SOUTHWEST NIGERIA
... aeruginosa of 38% in swimming pools and 73% of Jacuzzis and spas. Varying rates of P. aeruginosa reflects different approaches to the maintenance of swimming pool waters (Barben, 2005). The prevalence of Pseudo- monas ...
14
Effects of the presence of ColE1 plasmid DNA in Escherichia coli on the host cell metabolism
... of plasmid DNA or bearing plasmid pOri1 (with a single ColE1 replication origin) or plasmid pOri2 (with double ColE1 replication origins), were cultured in a ...number plasmid relative to E. ...
18
A plasmid-based Escherichia coli gene expression system with cell-to-cell variation below the extrinsic noise limit
... low cell-to-cell variability (noise) and expression levels precisely tunable across a useful dynamic ...a plasmid-based system incorporating these principles and studied its properties in Escheri- ...
15
Construction, Expression and Preliminary Immunological Evaluation of a DNA Plasmid Encoding the GRA2 Protein of Toxoplasma gondii
... We have focused on the development of a DNA- based vaccine because such vaccines have been shown to elicit a potent, long-lasting humoral and cell-mediated immunity, as well as providing protection against viral, ...
8
Bacterial Replicon: Construction of a Mini-F'km Plasmid
... Approximately 5 ,ug of the li- gated mixture of the EcoRI endonuclease-gen- erated fragments of the DNA of the sex factor F'lac, isolated from C600 recA trpR - trpE (F'lac), and the puri[r] ...
6
Analysis and comparison of plasmid profiles of Borrelia burgdorferi sensu lato strains
... The strains all contained a single large plasmid of 50 to ...garinii strains that contained two or three of the large ...stricto strains ranged in size from ...garinii strains with ...
7
Plasmid and surface antigen markers of endemic and epidemic Legionella pneumophila strains
... Environmental and clinical isolates of Legionella pneumophila obtained from the Pittsburgh Veterans Administration Medical Center were studied for the presence of plasmids and for unique[r] ...
6
Design and Construction Modifications Of switch reluctance motor
... Chapter 3 represents the most important part of this thesis. Here, the main calculation models, design algorithms and programs applied by author are presented. Algorithms of calculation of parameters and operation SRM ...
161