Point mutations that affect yGcn5 activity
Calmodulin Point Mutations Affect Drosophila Development and Behavior
... tions that give adult survivors in the hemizygous condi- tion ( Cam4" and Cam3") have a detectable maternal effect on egg hatch rate (Table 4), and several mutant [r] ...
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Novel point mutations attenuate autotaxin activity
... The role of particular amino acids within ATX in this enzyme mechanism is incompletely understood. Studies using chimeric constructs with regions from other NPPs indicate that regions throughout the entire length of ATX ...
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Mutations That Affect Dimer Formation and Helicase Activity of the Hepatitis C Virus Helicase
... These data indicate that dimerization of the helicase is important for helicase activity. The mutations that reduce self-association of the helicase may define the key residues involved in NS3-NS3 ...
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Mutations of the domain forming the dimeric interface of the ArdA protein affect dimerization and antimodification activity but not antirestriction activity
... ArdA activity via site- directed mutagenesis. The antirestriction activity of ArdA was maintained when up to seven mutations per monomer were made or the interface was disrupted such that the protein ...
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Analysis of coliphage lambda mutations that affect Q gene activity: puq, byp, and nin5.
... When potential recombinant plaques for per- tinent mutations were screened, the following pheno- types were used: (i) c1ts857, clear plaques at 420C; (ii) by, clear plaques at 32°C and t[r] ...
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A computational approach to identify point mutations associated with occult hepatitis B: significant mutations affect coding regions but not regulative elements of HBV
... Results: Variations in seven nucleotide positions were significantly associated with OBI, and occurred in 11 out of 41 OBI sequences (26.8%): likely, other mutations did not reach statistical significance due to ...
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Hypertension associated point mutations in the adducin alpha and beta subunits affect actin cytoskeleton and ion transport
... differentially affect actin cytoskeleton as- sembly and transmembrane ion ...pump activity in rat epithelial cell lines transfected with normal or mutated a -adducin ...
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Mutations in the membrane-spanning domain of the human immunodeficiency virus envelope glycoprotein that affect fusion activity.
... Subsequent mutagenic analysis of the HIV-1 Env transmembrane domain revealed that the sequence of amino acid residues from positions 696 to 707 of the transmembrane domain was important [r] ...
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Linker insertion mutations in the adenovirus preterminal protein that affect DNA replication activity in vivo and in vitro.
... Eighteen linker insertion mutants with mutations in the adenovirus precursor to terminal protein (pTP), which were originally constructed and tested in virions by Freimuth and Ginsberg ([r] ...
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Mutations in the PA Protein of Avian H5N1 Influenza Viruses Affect Polymerase Activity and Mouse Virulence
... PA affect virus replication and pathogenicity are not ...polymerase activity and pathogenicity of recombinant influenza viruses (28), is located on the same ␣-helix as residues 343 and ...
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Vesicular stomatitis virus glycoprotein mutations that affect membrane fusion activity and abolish virus infectivity.
... pAR-G. Mutations were confirmed by dideoxynucleotide sequencing of several individual clones for each of the mutant constructs, using Sequenase (United States Biochemical ...other mutations were introduced ...
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Single point mutations may affect the serotype reactivity of serotype G11 porcine rotavirus strains: a widening spectrum?
... Although it has been reported that conversion from one monotype to another may occur with a single amino acid change (13), the same has yet to be proven for G serotype specificity. Our novel results suggest that the ...
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Point Mutations Upstream of Hepatitis B Virus Core Gene Affect DNA Replication at the Step of Core Protein Expression
... Do mutations at nucleotides 1862, 1863, and 1869 just down regulate core protein expression? The G1862T, T1863A, and C1869A mutants are hampered in genome replication and core protein ...that mutations at ...
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Investigation Of Mutations In Nuclear Genes That Affect The Atp Synthase
... LDH activity as described under “Materials and ...LDH activity in each fraction was determined spectrophotmetrically by following the decrease in NADH absorbance at 340 nm for 2 min and the slopes ( ∆ ...
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Point mutations within the betaG-betaH loop of foot-and-mouth disease virus O1K affect virus attachment to target cells.
... cDNA plasmid. Transfection of baby hamster kidney cells (BHK-21) with in vitro-transcribed cRNAs contain- ing mutations bordering the RGD sequence led to the production of infectious virus in most cases. In ...
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Point Mutations in the Paramyxovirus F Protein That Enhance Fusion Activity Shift the Mechanism of Complement-Mediated Virus Neutralization
... fusion activity of the PIV5 F protein can dramatically alter complement activation in the context of viral particles or on the surface of virus-infected cells, shifting the acti- vation from the alternative ...
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Point mutations in the herpes simplex virus type 1 Vmw110 RING finger helix affect activation of gene expression, viral growth,
... Two zinc atoms are coordinated at either end of the helix, which extends basic and polar side chains into solvent. We surmised that these hydrophilic side chains would be required for the function of the herpesvirus RING ...
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Mutations in Classical Swine Fever Virus NS4B Affect Virulence in Swine
... or mutations in both box 1 and box 2 (IYK and VGI), was less efficient at mediating this reduction ...antiviral activity did not affect BICv rep- lication as it affected ...
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Analysis of fluG Mutations That Affect Light-Dependent Conidiation in Aspergillus nidulans
... fluG mutations that suppress the that production of the extracellular factor requires that veA1 phenotype suggests a genetic interaction between a strain contain a wild-type fluG allele or an allele that these two ...
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Single point mutations reveal amino acid residues important for Chromobacterium violaceum transaminase activity in the production of unnatural amino acids
... The crystal structure of CV_TA has been solved 17,18 . CV_TA is an (S)-enantioselective enzyme of approxi- mately 100 kDa, that prefers amino group donors with an aromatic ring over aliphatic amines and amino acids 16 . ...
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