Polo-like kinase 4
Polo-like kinase 4 at the nexus of epigenetic modifications and the DNA damage signaling network
... with Polo-like kinase 4 Nucleophosmin/B23 (NPM/NPM1), also called numatrin or NO38, is a ubiquitous nucleolar phosphoprotein from the NPM family of proteins which also include NPM2 and NPM3 ...
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Polo-like kinase 4: the odd one out of the family
... the kinase more than deletion of the two car- boxy-terminal PEST sequences, suggesting that it has a greater influence in controlling PLK4 stability ...(Figure 4) ...
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Polo like kinase confers MPF autoamplification competence to growing Xenopus oocytes
... Plx1 kinase activity (Karaiskou et ...Cdc2 kinase activity and analyzing Cdc25 phosphatase and Myt1 kinase phosphorylation states, or directly the Tyr15 phosphorylation of Cdc2 ...H1 kinase ...
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Targeting polo-like kinase 1, a regulator of p53, in the treatment of adrenocortical carcinoma
... membranes in 5 % BSA (1× TBST + 5 % BSA) overnight at 4 °C. Primary antibodies to MDM2 (AbCam, Cam- bridge, MA, USA) and p53 (AbCam, Cambridge, MA) at a dilution of 1:500 were added to the membranes in 1 % ...
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Polo-Like Kinase Guides Cytokinesis in Trypanosoma brucei through an Indirect Means
... tip” by localizing TbCPC1-EYFP and comparing it with the DAPI stain. In the no-drug control, TbCPC1-EYFP was iden- tified mostly in a punctate distribution in the nucleus at the beginning (0 h), representing the ...
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MICROWAVE ASSISTED SYNTHESIS AND MOLECULAR MODELING STUDIES OF SOME NOVEL POLO-LIKE KINASE 1 INHIBITORS AS ANTICANCER AND ANTI-INFLAMMATORY AGENTS
... A series of 1, 3-thiazolidin-4-one analogues were synthesized using microwave and structurally characterized by various spectroscopic techniques and elemental analysis. All synthesized compounds were screened for ...
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The activity regulation of the mitotic centromere-associated kinesin by Polo-like kinase 1
... This assay was performed with modifications as previously described [34]. In brief, coverslips with cells were incubated for 45 min in an ice bath to depolymerize microtubules. To allow microtubule regrowth, cells in ...
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Polo-Like Kinase 1 Is Involved in Hepatitis C Virus Replication by Hyperphosphorylating NS5A
... Plasmids and viruses. All the plasmids required for lentivirus production were provided by the National RNAi Core Facility, Academia Sinica, Taiwan. The four pLKO.1-shRNA vectors used for knockdown of Plk1 are the ...
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Characterization of the Sak kinase interaction with cyclin B1 and Gadd45a
... Polo-like Kinases Function and Members 1 The Structure of the Polo-like Kinases 2 Plks Expression and Kinase Activity 4.. Plks Localization 5.[r] ...
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A TAT-conjugated peptide inhibitor of polo-like kinase 1 for in vivo tumor imaging
... For in vivo optical imaging, 2 was injected into HeLa tumor-bearing mice xenograft to determine the tumor-tar- geting and diagnosis effectiveness. Samples were analyzed at four time points, namely pre-injection, 3 h, 6 ...
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Exploration of Mutation and DNA Methylation of Polo Like Kinase 1 (PLK1) in Colorectal Cancer
... a. The methylation status (fraction of methylated CpG) of the CpG islands was detected by mass spectrometry. Samples are named according to the se- quence of the cell line (C, H, S and T refer to Colo320DM, HCT116, SW48 ...
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Mumps Virus Nucleoprotein Enhances Phosphorylation of the Phosphoprotein by Polo-Like Kinase 1
... The P proteins of paramyxoviruses are highly phosphorylated, and phosphorylation of these proteins has been shown to play critical roles in regulating viral mRNA synthesis (2–7). Phosphor- ylation of residues within the ...
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Co-inhibition of polo-like kinase 1 and aurora kinases promotes mitotic catastrophe
... completely inhibited using relatively high concentrations of the drugs. As described above, inhibition of PLK1 induced a metaphase arrest (Figure 2; Supplementary Video 3). Inhibition of AURKA causes defects in ...
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Pooled shRNA screen for sensitizers to inhibition of the mitotic regulator polo-like kinase (PLK1)
... RNAi screening holds the promise of systemizing the search for combination therapeutic strategies. Here we performed a pooled shRNA library screen to look for promising targets to inhibit in combination with inhibition ...
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Polo-Like Kinase 1 as a Target for Human Cytomegalovirus pp65 Lower Matrix Protein
... N-terminal kinase domain alone could not, and that the deletion of an hydrophilic tract within pp65 protein abolished the interaction ...cell kinase was found to be coimmunoprecipitated with a recom- binant ...
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Polo-Like Kinase 1 phosphorylates and stabilizes KLF4 to promote tumorigenesis in nasopharyngeal carcinoma
... Krüppel-like factor 4 (KLF4) is an important regulator of cell fate decisions, including the DNA damage response, inflammation, apoptosis and stem cell renewal. KLF4 has been reported to have ...
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Polo-like kinase 2 acting as a promoter in human tumor cells with an abundance of TAp73
... at 4 ° C for 12 ...at 4 ° C for 12 hours with TAp73 (1:100), PLK2 (1:200), flag (1:200) antibody, 20 μ L protein G + /protein A agarose (Oncogene Research Products), or anti-flag affinity agarose gel ...
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Hyper-activation of Aurora kinase a-polo-like kinase 1-FOXM1 axis promotes chronic myeloid leukemia resistance to tyrosine kinase inhibitors
... A single genetic lesion, the t (9,22) reciprocal translocation which generates the BCR-ABL1 rearranged gene, is the causative event of CML, driving clonal expansion of leukemic hematopoiesis via the constitutive ...
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Impact of Polo-like kinase 1 inhibitors on human adipose tissue- derived mesenchymal stem cells
... The Polo-like kinase (Plk) family is a group of highly conserved serine/threonine ...N-terminal kinase domain and the C-terminal regulatory Polo-box domain (PBD), which offer multiple ...
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The balance of Polo-like kinase 1 in tumorigenesis
... gression. Plk1 localizes at the centrosomes during inter- phase and prophase, and is found at kinetochores in pre- metaphase and metaphase. It later relocates to spindles during anaphase and finally resides at the ...
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