Poly [ADP-Ribose] Polymerase 2
Poly(ADP-ribose) polymerase 2 contributes to neuroinflammation and neurological dysfunction in mouse experimental autoimmune encephalomyelitis
... both PARP-1 and PARP-2 [21-23], and we (data not shown) and others [14] found that PARP-1 deletion is not protective but rather enhances peak disease severity. How- ever, PARP-1 deletion reduced neuroinflammation ...
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Original Article Polymorphisms in poly (ADP-ribose) polymerase-1 (PARP1) promoter and 3’ untranslated region and their association with PARP1 expression in breast cancer patients
... The immunohistochemistry was independently assessed by two pathologists who were blinded to PARP1 genotypes and clinico-pathological data. In cases of disagreement, the result was resolved by consensus. We employed the ...
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9 Phenyl Acridine: A Possible Poly (ADP ribose) Polymerase 1 Inhibitor
... some differences as observed in case of some other proteins in PDB [31]. Thus we tried to analyze the three dimensional structure of PARP1 from human to analyze its mode interactions with the ligand. Our findings ...
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Update on Poly ADP ribose polymerase inhibition for ovarian cancer treatment
... In the trial by Drew et al. [65] i.v. rucaparib was admin- istered daily for 5 days every 21 days, in patients with breast and EOC. The trial was divided in two stages: stage 1 was a short dose-escalation phase; in stage ...
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Small PARP inhibitor PJ-34 induces cell cycle arrest and apoptosis of adult T-cell leukemia cells
... and poly (ADP-ribose) polymerase (PARP) is a substrate of caspase-3 protease activity and its processing is usu- ally associated with apoptotic cell ...
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Rhein sensitizes human pancreatic cancer cells to EGFR inhibitors by inhibiting STAT3 pathway
... Cleaved poly ADP-ribose polymerase (Cl-PARP), B-cell lymphoma 2 (BCL-2), BCL-2-associated X protein (BAX), Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), horseradish ...
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Combination of erlotinib and a PARP inhibitor inhibits growth of A2780 tumor xenografts due to increased autophagy
... Results: Erlotinib could slightly inhibit growth of A2780 tumor xenografts, and AZD2281 alone had similar effects on tumor growth. However, the combination treatment had a markedly enhanced antitumor effect. Western blot ...
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Poly(ADP-Ribose) Polymerase Is a Substrate Recognized by Two Metacaspases of Podospora anserina
... In order to investigate the effects of metacaspase-mediated rPaPARP cleavage (Fig. 4C and D), we tested whether rPaPARP is still functional after incubation with rPaMCA1 or rPaMCA2. Prior to detection of PARP activity, ...
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Noncleavable poly(ADP ribose) polymerase 1 regulates the inflammation response in mice
... EDTA, 1 mM DTT, 0.1% NP-40 and protease inhibitors). Human THP-1 cells were treated with 2 μg/ml LPS for the indicated time. Protein samples (25 μg) were resolved on a 10% SDS-PAGE and blotted onto a nitrocel- ...
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Poly(ADP-ribose)polymerase-1 modulates microglial responses to amyloid β
... and 2 mM glutamine, and plated on poly-D-lysine- coated 24-well plates at a density of 7 × 10 5 cells per ...After 2 days in vitro, 22 μM cytosine b-D-arabino- furanoside (Sigma, ...
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Poly (ADP-ribose) polymerase 3 (PARP3), a potential repressor of telomerase activity
... In relation to telomerase activity, 24 hours post- transfection no differences were found between trans- fected cells with pcDNA/GW-53/PARP3 and transfected cells with the empty vector. Telomerase activity average ratio ...
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Poly(ADP-Ribose) Polymerase 1 Promotes Transcriptional Repression of Integrated Retroviruses
... Poly(ADP-ribose) polymerase 1 (PARP-1) is a key cellular en- zyme in DNA repair and chromatin-remodeling processes (13, ...of ADP ribose molecules from NAD ⫹ to acceptor proteins ...
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CYTOTOXICITY AND IN SILICO STUDIES OF ANETHOLE IN TRIPLE NEGATIVE BREAST CANCER CELLS
... receptor 2 are ...as poly-ADP-ribose and insulin like growth factor receptor were investigated in the present ...namely poly ADP-ribose polymerase (PARP) and ...
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Disruption of Poly(ADP-ribose) Polymerase (PARP) Protects Against Stress-Evoked Immunocompromise
... Indeed, it is well known that both psychological and physical stressors modulate the hypothalamic- pituitary-adrenal (HPA) axis, leading to increased glucocorticoid release which, in turn, modulates immune function in ...
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Zinc Inhibits Astrocyte Glutamate Uptake by Activation of Poly(ADP-ribose) Polymerase-1
... study, cultured cortical astrocytes were used to explore the effects of zinc on astrocyte glutamate uptake, an energy-depend- ent process that is critical for neuron survival. Astrocytes incubated with 100 or 400 µM of ...
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Angiotensin II-Mediated Endothelial Dysfunction: Role of Poly(ADP-ribose) Polymerase Activation
... 20 min at 37 °C. The cells were then scraped from the wells and placed in Eppendorf tubes containing 200 µL ice-cold 50% trichloroacetic acid (TCA) (w/v). The tubes were then placed at 4 °C. After 4 h, the tubes were ...
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Poly(ADP-ribose) Polymerase is a Regulator of Chemokine Production: Relevance for the Pathogenesis of Shock and Inflammation
... J774 cells were pretreated with PJ-34 (30 M) or its vehicle for 30 min, which was followed by a 3-hr exposure to LPS (10 g/ml). At the end of the incuba- tion period, total cellular RNA was extracted and chemokine mRNA ...
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Poly(ADP-Ribose) Polymerase-1 Inhibition: Preclinical and Clinical Development of Synthetic Lethality
... well tolerated, and no maximum toler- ated dose was identified. The most com- mon adverse events were gastrointestinal. To determine the safety and maximum tolerated dose of BSI-201 in combination with other ...
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Review Article Potential biomarkers of Poly (ADP-ribose) polymerase inhibitors for cancer therapy
... To investigate if this sensitivity works in vivo, BRCA-deficient ES cells [32] or BRCA2-deficient V-C8 [31] cells were injected into nude mice. The result showed that significant differences in tumor formation were seen ...
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Poly(ADP-Ribose) Polymerase 1 Is Not Strictly Required for Infection of Murine Cells by Retroviruses
... Infection of wild-type and PARP-1-deficient MEF cells with MLV. To examine the effect of infection with a more biologi- cally relevant retrovirus, MEFs were incubated with three dif- ferent input amounts of MLV and ...
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