polysaccharide monooxygenase
An actinobacteria lytic polysaccharide monooxygenase acts on both cellulose and xylan to boost biomass saccharification
... LPMO: lytic polysaccharide monooxygenase; AA9: auxiliary activity 9; AA10: auxiliary activity 10; AA14: auxiliary activity 14; CAZy: carbohydrate active enzymes databank; CBP21: chitin[r] ...
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Activity, stability and 3-D structure of the Cu(II) form of a chitin-active lytic polysaccharide monooxygenase from Bacillus amyloliquefaciens
... Page 11 of 22 Dalton Transactions Activity, stability and 3-D structure of the CuII form of a chitin-active lytic polysaccharide monooxygenase from Bacillus amyloliquefaciens Rebecca C G[r] ...
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Real-time imaging reveals that lytic polysaccharide monooxygenase promotes cellulase activity by increasing cellulose accessibility
... DP3 were detected in all replicates of TrAA9A‑containing samples. Lytic polysaccharide monooxygenase (LPMO) degrades cellulose to cello‑ oligosaccharides and oxidized cello‑oligomers. Three types of LPMOs ...
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Discovery of the combined oxidative cleavage of plant xylan and cellulose by a new fungal polysaccharide monooxygenase
... lytic polysaccharide monooxygenase (MtLPMO9A) that shows oxidative cleavage of xylan in addition to cellulose and that acts in synergism with endoglucanase ...plant polysaccharide matrix in plant ...
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Structure of a Thermobifida fusca lytic polysaccharide monooxygenase and mutagenesis of key residues
... Fig. 1 Structure of T. fusca AA10A and the domain arrangement of T. fusca LPMOs. a Overall structure of TfAA10A shown as a ribbon with red α‑helices, yellow β‑sheets and green loops with a surface representation ...
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Activity, stability and 3-D structure of the Cu(II) form of a chitin-active lytic polysaccharide monooxygenase from Bacillus amyloliquefaciens
... that the peaks for the dual Na + form are always stronger than the other two for each DP, reflecting the presence of NaCl in the protein buffer prior to dilution in the reaction. Consistent with other work 13, 19, 20 , ...
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AA16, a new lytic polysaccharide monooxygenase family identified in fungal secretomes
... aculeatus AA16 LPMO characterized in this study shares many features with fungal LPMOs from other families, including (i) two strictly conserved histidines forming the copper histidi[r] ...
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Polysaccharide monooxygenase-catalyzed oxidation of cellulose to glucuronic acid-containing cello-oligosaccharides
... HiPMO1 oxidizes C6 of cellulose to form glucuronic acid-containing cello-oligosaccharides followed by hydrolysis with beta-glucosidase and beta-glucuronidase to yield glucose, glucuro[r] ...
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Activity, stability and 3-D structure of the Cu(II) form of a chitin-active lytic polysaccharide monooxygenase from Bacillus amyloliquefaciens
... Studies into the likely mechanism of action used by LPMOs have centred on understanding their copper active site geometries 7-9, 23-25 . All LPMOs characterised to date bind a single copper ion in their active sites via ...
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Influence of the carbohydrate-binding module on the activity of a fungal AA9 lytic polysaccharide monooxygenase on cellulosic substrates
... The reaction mixtures were carried out at 0.3% (w/v) insoluble substrate loading (BMCC; NFC; PASC) and 30 µg of proteins were added. The reactions were done in 50 mM sodium acetate buffer pH 5.2 in a final volume of 200 ...
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Structural Investigations of the Fungal Lytic Polysaccharide Monooxygenase Reaction Mechanism using X-ray and Neutron Protein Crystallography.
... The X-ray and neutron protein crystal structures of Nc LPMO9D described in 3.1 and 3.2 demonstrate an important contribution to fungal LPMO reactivity arising from residues in the “se[r] ...
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The Podospora anserina lytic polysaccharide monooxygenase PaLPMO9H catalyzes oxidative cleavage of diverse plant cell wall matrix glycans
... or three (DP4) adjacent β-(1 → 4) linkages are predomi- nant, while longer cellodextrin sequences contributed to less than 10% of the total polysaccharide chain in the starchy endosperm [23]. If one assumes that ...
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Improved spectrophotometric assay for lytic polysaccharide monooxygenase
... Background: The availability of a sensitive and robust activity assay is a prerequisite for efficient enzyme production, purification, and characterization. Here we report on a spectrophotometric assay for lytic ...
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Regioselectivity of oxidation by a polysaccharide monooxygenase from Chaetomium thermophilum
... PMOs: polysaccharide monooxygenases; CtPMO1: a PMO from Chaetomium thermophilum; PASC: phosphoric acid-swollen cellulose; TFA: trifluoroacetic acid; AA: auxiliary activity; PCR: polymerase chain reaction; ...
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A fast and sensitive activity assay for lytic polysaccharide monooxygenase
... with polysaccharide analysis by carbohydrate gel electro- phoresis (PACE), has been reported to yield mostly cel- lobiose and cellotriose ...chromogenic polysaccharide hydrogel substrates were used to ...
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Improving extracellular production of Serratia marcescens lytic polysaccharide monooxygenase CBP21 and Aeromonas veronii B565 chitinase Chi92 in Escherichia coli and their synergism
... Lytic polysaccharide monooxygenases (LPMOs) can oxidize recalcitrant polysaccharides and boost the conversion of the second most abundant polysaccharide chitin by ...
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Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase
... a polysaccharide chain within the more contoured surface of a retrograded starch 36 or possibly a single glucan chain (see Supplementary Discussion), as compared with the flat crystalline surfaces of b-linked ...
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Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase
... Stereo view divergent, ‘wall-eyed’ of the putative substrate-binding faces of a AoAA13 depicting shallow groove leading through copper active site N-terminal histidine shown in blue, cop[r] ...
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Expression and secretion of a lytic polysaccharide monooxygenase by a fast-growing cyanobacterium
... export and secretion pathways in cyanobacteria have been studied for almost two decades, they remain poorly understood (see recent reviews [21, 23]). Proof-of-con- cept studies have shown that heterologous protein secre- ...
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Production and spectroscopic characterization of lytic polysaccharide monooxygenases
... Fungal cellulose degradation by oxidative enzymes: from dysfunctional GH61 family to powerful lytic polysaccharide monooxygenase family.. Production of first and second generation biofue[r] ...
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