Primer-probe sets for quantitative RT-PCR
Real-time quantitative RT -PCR (Taqman)
... d) Primer optimisation Reactions to amplify two different segments in the same tube share common reagents. If the two segments have different initial copy numbers, it is possible for the more abundant species to ...
6
A plastome primer set for comprehensive quantitative real time RT-PCR analysis of Zea mays: a starter primer set for other Poaceae species
... ble quantitative data [Figure ...these primer sets and amplification conditions eliminated the need for additional primer design for these ...the primer pairs have been optimized for ...
9
Evaluation of Twelve Real Time Reverse Transcriptase PCR Primer Probe Sets for Detection of Pandemic Influenza A/H1N1 2009 Virus
... these primer-probe sets are shown in Table ...these primer-probe sets were based on original reports (7, 11, 18, 24, 27, ...of PCR cycles was set at 45, which was the ...
7
Use of multiple PCR primer sets for optimal detection of human papillomavirus
... of PCR detection with consensus HPV primers is 4,200 SiHa cells (6,300 HPV-16 DNA ...of PCR sensitivity for a few copies of HPV DNA in clinical samples may be misleading, since the determinations were not ...
6
Development of a highly sensitive real time one step RT PCR combined complementary locked primer technology and conjugated minor groove binder probe
... of primer provided to increase sensitivity. Our data sug- gest that TMC-PCR assay may be more suitable to rou- tine diagnostic assay for EV detection from clinical specimens even though TMC-PCR assay ...
6
quantitative real-time PCR, grain, simplex DNA extraction: PGS0426 RT-PCR: PGS0494 & PGS0476
... amplified. PCR products are measured during each cycle (real-time) by means of a Target-specific oligonucleotide probe labeled with two fluorescent ...
10
Application of PCR primer sets for detection of Pseudomonas sp functional genes in the plant rhizosphere
... a PCR primer sets targeting genes encoding the main antibiotic ...that primer sets for phlD and phzCD detected these genes in a fal- lowed neutral pH soil following wheat produc- tion, ...
8
Reference gene identification for reliable normalisation of quantitative RT-PCR data in Setaria viridis
... for RT- qPCR data normalisation, these two sets of putative candidate reference genes were used to generate an expression profile for two members of the ...two sets of candidate reference ...
12
Application of real-time quantitative RT-PCR for improving the diagnosis, treatment, and control of bovine Anaplasmosis
... Several polymerase chain reaction (PCR) methods have been developed to identify DNA of A. marginale (Bekker et al., 2002; Eriks et al., 1989; French et al., 1998; Gale et al., 1996b; Ge et al., 1995; Molad et al., ...
167
A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors
... given primer pair is doubled in every PCR cycle of the exponential ...the primer, and the unwanted formation of primer-dimers are factors that can significantly affect the efficiency of indi- ...
9
Diffuse large B-cell lymphoma: sub-classification by massive parallel quantitative RT-PCR
... the probe annotation was updated according to Release 33 (30/10/2012), while for GEP dataset by Illumina WG-DASL assay, the specificity of the classifier gene probes was further checked by search of the NCBI human ...
26
Evaluation of reference genes in mouse preimplantation embryos for gene expression studies using real time quantitative RT PCR (RT qPCR)
... We confirmed the amplification of specific RT-qPCR products by performing a melting-curve step at the end of each run. Serial dilution curves for each primer allowed us to calculate RT-qPCR ...
12
HiPer RT-PCR Teaching Kit
... for RT-PCR, as probe for expression analysis and for cloning of the mRNA ...In RT-PCR three major steps are ...(M-MLV RT) is an RNA-dependent DNA polymerase that can be used in ...
9
An Interactive 3D Visualization Tool for Large Scale Data Sets for Quantitative Atom Probe Tomography
... data sets of atom probe ...for quantitative analysis of chemical composition within the ...data sets in real time, and suggests a solution to this problem in terms of already existing ...
5
Superiority of Digital Reverse Transcription PCR (RT PCR) over Real Time RT PCR for Quantitation of Highly Divergent Human Rhinoviruses
... consensus primer and probe binding regions, not all HRV genotypes were tested, especially the HRV species C ...that RT-dPCR quantification of HRV RNA will be useful in future HRV viral quantification ...
8
Development of a real time RT PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland
... same sets of primers are used for both assays the combination of both methods greatly speeds up ascer- taining when a NoV outbreak is occurring and which strain is ...the RT-PCR products to be ...
8
Classification and risk stratification of invasive breast carcinomas using a real time quantitative RT PCR assay
... See http://www.biomedcentral.com/content/ supplementary/bcr1399-S1.doc Additional File 2 Supplemental Table 1. Clinical data for the 123-sample set prospectively analyzed by microarray and qRT-PCR Supplemental ...
11
Determination of the exact copy numbers of particular mRNAs in a single cell by quantitative real time RT PCR
... the probe was 5 ′ -FAM- TTT TCA ATG TCA GCT GTT CCA GGA CCA T-TAMRA- 3 ′ ...the probe was 5 ′ - FAM-CTG CCA AGC AGC AAA TCT TCG TTC CA- TAMRA-3 ′ ...
10
Introduction to Quantitative PCR
... a primer/probe set specific to each allele run either side by side on the same sample or in ...standard Quantitative Plate Read experiment using either endpoint fluorescence alone or fluorescence ...
114
Identification of Genetic Evidence for Dobrava Virus Spillover in Rodents by Nested Reverse Transcription (RT) PCR and TaqMan RT PCR
... TaqMan RT-PCR assay was tested with a syn- thetic RNA standard transcribed from a DOBV S segment ligated into pCRII (Invitrogen, Breda, The ...TaqMan RT-PCR to specifically detect the extreme ...
5