procollagen-C
Gene Interactions in Caenorhabditis elegans Define DPY-31 as a Candidate Procollagen C-Proteinase and SQT-3/ROL-4 as Its Predicted Major Target
... Figure 1.—Phenotype of dpy-31 mutants. (A) WT adult worm. (B) dpy-31(e2770) adult raised at 15⬚. (C) e2770 embryos raised at 25⬚: the cuticle surface in tail region of a dead embryo (solid arrow) appears abnormal. ...
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Serum concentrations of carboxyl terminal propeptide of type I procollagen, amino terminal propeptide of type III procollagen, cross linked carboxl terminal telopeptide of type I collagen, and their interrelationships in schoolchildren
... for procollagen type I C-terminal propeptide (PICP), the cross-linked C-terminal telopeptide of type I collagen (ICTP), and procollagen type III N-terminal propeptide (P3NP), measured in ...
5
A mutation in the gene for type III procollagen (COL3A1) in a family with aortic aneurysms
... III procollagen to a codon for ...III procollagen that had a decreased temperature for thermal unfolding of the ...III procollagen gene can cause familial aortic aneurysms ...
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Helical mutations in type I collagen that affect the processing of the amino-propeptide result in an Osteogenesis Imperfecta/Ehlers-Danlos Syndrome overlap syndrome
... the procollagen type I-N-proteinase cleavage site ...processed procollagen chains, in which the C-propeptide but not the N-propeptide has been cleaved off, are secreted and incorporated into the ...
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Comparative proteomic analysis of extracellular matrix proteins secreted by hypertrophic scar with normal skin fibroblasts
... The formation of hypertrophic scars (HSs) is a fibroproliferative disorder of abnormal wound healing. HSs usually characterize excessive proliferation of fibroblasts, abnormal deposition of extracellular matrix (ECM) ...
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Transforming growth factor beta increases steady state levels of type I procollagen and fibronectin messenger RNAs posttranscriptionally in cultured human dermal fibroblasts
... I procollagen chains 3-5-fold as shown by polypeptide ...I procollagen and fibronectin also occurred but without a net increase in the rate of transcription of either of these ...I procollagen and ...
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Effect of thrombin and alpha-1-antitrypsin on mesenchymal cell proliferation and procollagen production
... (T) procollagen cDNA or GAPDH (glyceraldehyde-phosphate dehydrogenase) obtained from the American Type Culture Collection) was labelled with f^P]-dCTP (Amersham International, Amersham, Buckinghamshire, UK) using ...
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Evidence for altered synthesis of type II collagen in patients with osteoarthritis
... the C-propeptide of type II procollagen (CPII), released extracellularly from the newly synthesized molecule, is directly related to the syn- thesis of this molecule in healthy and osteoarthritic articu- ...
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The role of MAP kinases in the regulation of mechanical load-induced procollagen gene expression in cardiac fibroblast
... kinase C inhibitors such as staurosporine, H7, bisindoylmaleimides, or by downregulating PKC by prolonged treatment with phorbol ...of c-fos gene expression (Komuro et al, ...load-induced procollagen ...
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Radioimmunoassay for the carboxy terminal cross linking domain of type IV (basement membrane) procollagen in body fluids Characterization and application to collagen type IV metabolism in fibrotic liver disease
... The carboxy-terminal cross-linking domain (NCl) of type IV procollagen was isolated from human placenta and used for the production of polyclonal and monoclonal antibodies. Purity of the antigen and specificity of ...
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Effect of mechanical load and polypeptide growth factors on cardiovascular fibroblast function
... For experiments assessing DNA synthesis by pH]methy 1-thymidine incorporation, l^Ci/well was added to each well in the incubation medium. Cells were loaded for up to 88 hours, with a labelling period of the final 4 ...
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A lethal variant of osteogenesis imperfecta has a single base mutation that substitutes cysteine for glycine 904 of the alpha 1(I) chain of type I procollagen The asymptomatic mother has an unidentified mutation producing an overmodified and unstable type I procollagen
... I procollagen synthesized by the proband's fibroblasts had a thermostability that was 3-4 degrees C lower than the normal type I procollagen as assayed by brief ...
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Restriction fragment length polymorphism associated with the pro alpha 2(I) gene of human type I procollagen Application to a family with an autosomal dominant form of osteogenesis imperfecta
... One cloned complementary DNA and one genomic subclone were used to detect restriction fragment length polymorphism associated with the pro alpha 2(I) gene for human type I procollagen. The restriction fragments ...
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Scavenger receptor mediated endocytosis in endocardial endothelial cells of Atlantic cod Gadus morhua
... of 200 µl per well. Incubations carried out at 12 °C for various times were terminated by transferring the incubation medium along with the first of three washes of 500 µl of PBS to tubes containing 800 µl of 20 % ...
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Genetic causes of aortic aneurysms Unlearning at least part of what the textbooks say
... Also, linkage studies suggested mutations in the gene for any genetic defect causing atherosclerosis or hypertension is type II procollagen in two families with osteoarthritis and a the [r] ...
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Effects of Er-Miao-San extracts on TNF-alpha-induced MMP-1 expression in human dermal fibroblasts
... Cellular proteins was extracted by lysis buffer (50 mM Tris–HCl, pH 8.0, 150 mM NaCl, 1% NP-40, 0.1% SDS, and 0.5% sodium deoxycholate) and EDTA-free protease and phosphatase inhibitor cocktail (Roche, Switzerland). ...
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Collagen gene expression by cultured human skin fibroblasts Abundant steady state levels of type VI procollagen messenger RNAs
... demonstrated the presence of specific mRNA transcripts for collagen types I, III, IV, V, and VI, but not for type II collagen. Quantitation of the abundance of these mRNAs by slot blot hybridizations revealed that type ...
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The role of fibrin in skin fibrosis
... of procollagen degradation occurs soon after synthesis and is likely to occur in the lysosomes, since inhibition of the lysosomal proteases cathepsins B, D and L, prevents intracellular procollagen ...
244
d alpha tocopherol inhibits collagen alpha 1(I) gene expression in cultured human fibroblasts Modulation of constitutive collagen gene expression by lipid peroxidation
... Ascorbic acid stimulates collagen gene transcription in cultured fibroblasts, and this effect is mediated through the induction of lipid peroxidation by ascorbic acid. Quiescent cultured fibroblasts in the absence of ...
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Epidermolysis bullosa acquisita antigen is the globular carboxyl terminus of type VII procollagen
... epidermis and dermis of skin, the site of blister formation. The "EBA antigen" is known to be distinct from laminin, heparan sulfate proteoglycan, fibronectin, the bullous pemphigoid antigen, elastin, and ...
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