Proliferation and Migration Assays
MicroRNA 135a regulates NHE9 to inhibit proliferation and migration of glioblastoma cells
... reporter assays, real-time PCR and western blotting were conducted to examine the expression profiles and identify microRNAs (miRNA) that target ...Cell proliferation and migration assays were ...
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Upregulation of circ_0066444 promotes the proliferation, invasion, and migration of gastric cancer cells
... and migration of gastric cancer cells For migration assays, a total of 1 × 10 5 infected cells were plated in the top chamber of Transwell assay inserts (Millipore, Billerica, MA, USA) with a ...
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CDK14 involvement in proliferation migration and invasion of esophageal cancer
... Our work explored the concrete mechanism of CDK14 in proliferation of ESCC. Western blots and immunohistochemistry (IHC) analysis verified that expression of CDK14 in tumor tissues is increased which is compared ...
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CCL11 promotes migration and proliferation of mouse neural progenitor cells
... Methods: Nine-day-old mice were exposed to a 120-minute hypoxia following unilateral carotid occlusion. Chemokine levels were quantified in mouse brain extract. Migration and proliferation assays ...
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Suppression of CCT3 inhibits the proliferation and migration in breast cancer cells
... Lentiviral vector construction and transduction For the construction of shRNA expression plasmids, shCCT3 was designed based on the target sequence 5 ′ -CAA GTC CAT GAT CGA AAT T-3 ′ . Then, the single strand DNA oligo ...
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Microglia enhanced the angiogenesis, migration and proliferation of co-cultured RMECs
... For immunofluorescence assays, cell cultures were fixed with 4% paraformaldehyde in 0.01 M PBS for 10 min and then washed with PBS. After washing, the cultures were incubated in blocking buffer (5% normal goat ...
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TTK activates Akt and promotes proliferation and migration of hepatocellular carcinoma cells
... cell migration, demethylation, Akt Received: April 19, 2015 Accepted: September 07, 2015 Published: September 19, 2015 ABSTRACT Hepatocellular carcinoma (HCC) is one of the most malignant cancers with poor ...
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Overexpression of NELFCD promotes colorectal cancer cells proliferation, migration, and invasion
... cell proliferation, migration, invasion, and apoptosis was detected by cell counting kit-8, plate colony formation assay, transwell migra- tion and invasion assays, and flow cytometry in vitro and by ...
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Knockdown of KIF26B inhibits breast cancer cell proliferation, migration, and invasion
... the migration and invasion of breast cancer cells, we conducted Transwell migration and Matrigel invasion ...cell migration and the invasive capabilities of KIF26B knockdown cells were significantly ...
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<p>MAGI1 Inhibits the Proliferation, Migration and Invasion of Glioma Cells</p>
... MTT assays were used to evaluate cell proliferation, Transwell assays to determine cell migration and invasion, and a xenograft model established using U87 cells to evaluate the effect of ...
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Cell proliferation and cytotoxicity assays
... and proliferation of cells is a vital indicator for understanding the mechanisms in action of certain genes, proteins and pathways in- volved cell survival or death after exposing to toxic ...cell ...
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RELIABILITY OF LYMPHOCYTE PROLIFERATION ASSAYS
... Using a doubly multivariate repeated measures design, significant time and concen- tration effects were observed for both parameters: concanavalin A induced blastogenesis (ConA) and p[r] ...
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15.4 Assays for Cell Enumeration, Cell Proliferation and Cell Cycle. Cell Enumeration and Cell Proliferation Assays for Animal Cells
... samples. Proliferation Assays Using Bromodeoxyuridine Incorporation Incorporation of 5-bromo-2 ′-deoxyuridine (BrdU, B-23151) into newly synthesized DNA permits indirect detection of rapidly proliferating ...
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Proliferation/Potency Assays: Is there more to CD34 and CFU?
... D6.1.3 There shall be the establishment of appropriate and validated assays and test procedures for the evaluation of cellular therapy products. D6.1.3.1 For all cellular therapy products, a total nucleated cell ...
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The role of proteoglycans in cell adhesion, migration and proliferation.
... Bacteria and Trypanosomes into Mammalian Cells. One of the tirst studies to demonstrate that a unique protein on the surface of pansites interacts a-ith HSPGs on [r] ...
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proliferation and migration in oral squamous cell carcinoma
... cell proliferation, CCK-8 assay was carried out at 12, 24, 48, and 72 hours after siL- INC00662 or LINC00662 transfection (Figure ...the proliferation of OSCC cells in ...
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Comparison of Human Immunodeficiency Virus Antigens as Stimulants for Lymphocyte Proliferation Assays
... onstrates a significant correlation with viral load (10) or de- cline in CD4-cell numbers (9) in HIV-infected subjects. LPAs have been the standard assays used to assess the CD4 response to antigenic stimuli. ...
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BORA regulates cell proliferation and migration in bladder cancer
... Conclusions: Our study indicates that BORA regulates BCa cell cycle and growth, meanwhile influences cell motility by EMT, and could be a novel biomarker and potential therapeutic target in BCa. Keywords: Bladder cancer, ...
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FOXP3, anovel glioblastoma oncosuppressor, affects proliferation and migration
... The major finding of this study, however, is the contribution of FOXP3 down-regulation to GB biology. We first verified that FOXP3 expression in GB-NS is not modulated by TGF-β, a factor playing a central role in the ...
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Targeting DYRK1B suppresses the proliferation and migration of liposarcoma cells.
... DYRK1B (also known as Mirk) belongs to the DYRK family of protein kinases, which include five conserved members, DYRK1A, DYRK1B, DYRK2, DYRK3, and DYRK4. DYRKs are dual function kinases family with the ability to ...
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