Protein sizes and cysteine residues of CEs
Importance of Conserved Cysteine Residues in the Coronavirus Envelope Protein
... conserved cysteine residues located on the carboxy side of the long hydrophobic domain, suggesting functional ...E protein is ...conserved residues and the necessity of palmitoylation, three ...
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Mutational analysis of the cysteine residues in the hepatitis B virus small envelope protein.
... Of four mutants involving the eight cysteines located in the antigenic region, only the double mutant lacking Cys.121 and Cys-124 was secreted with wild-type efficiency.. Secretion-compe[r] ...
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Cysteine associated distribution of aromatic residues in disulfide stabilized extracellular protein families
... work residues in examining large sets of Immunoglobu- lin variable (IgV) domains ...selected cysteine-associated sites of preferred amino acid chemistry shared within and across modern protein ...
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Relevance of cysteine residues for biosynthesis and antigenicity of human hepatitis B virus e protein.
... Mutation of Cys-61 reduces HBe antigenicity. One impor- tant characteristic of the HBe protein is that it is serologi- cally distinct from the other major core gene product, the HBc prot[r] ...
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Cysteine residues in the Vif protein of human immunodeficiency virus type 1 are essential for viral infectivity.
... Immunoblot analysis of gp4l present in viral particles produced in CEM cells by wild-type and vif mutant HIV-1. Virus was[r] ...
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Alteration of Sendai Virus Morphogenesis and Nucleocapsid Incorporation due to Mutation of Cysteine Residues of the Matrix Protein
... (M) protein of Sendai virus (SeV) has five cysteine residues, at positions 83, 106, 158, 251, and ...the cysteine residues in viral assembly, we generated mutant M cDNA possessing a ...
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Measles Virus Fusion Protein Is Palmitoylated on Transmembrane-Intracytoplasmic Cysteine Residues Which Participate in Cell Fusion
... candidate cysteine residues, which may be ...palmitoylated cysteine residues of MV F protein, we have investigated palmitate incorporation into wild-type and mutant F proteins in which ...
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Contribution of cysteine residues in the extracellular domain of the F protein of human respiratory syncytial virus to its function
... of cysteine residues 37, 313, 322, 333, 343, 358, 367, 393, 416, and 439 reduced binding of all four antibodies, while mutation of cysteine mutants C382S, and C422S retained similar levels of ...
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Mapping of functionally important residues of a cysteine-histidine box in the human immunodeficiency virus type 1 nucleocapsid protein.
... Amino acid sequences of the proximal (amino acids 15 to 28) and distal (amino acids 36 to 49) cysteine-histidine boxes in the HIV-1 nucleocapsid protein and nature of the introduced muta[r] ...
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Pairs of Vp1 Cysteine Residues Essential for Simian Virus 40 Infection
... capsid protein Vp1 ...Vp1 cysteine pairs during SV40 ...Vp1 cysteine residues functions at two stages of SV40 infection: during Vp1 folding and oligomerization in the cytoplasm and during ...
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Mutation of Neuraminidase Cysteine Residues Yields Temperature-Sensitive Influenza Viruses
... C303G and C320G fail to produce stable, properly folded NA at the nonpermissive temperature. To better understand the specific defect in the C303G and C320G viruses, we exam- ined viral protein expression levels ...
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Palmitoylation and membrane interactions of cysteine string protein
... f cysteine residues through a thioester ...regulating protein trafficking, the membrane m icro-localisation o f proteins, protein stability, and protein-protein ...DHHC ...
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Machine learning methods for prediction of disulphide bonding states of cysteine residues in proteins
... the cysteine bonding state with flanking ...free cysteine propensities, respectively, with no hidden ...2452 cysteine residues containing segments extracted from 641 non-homologous proteins of ...
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Structure–function analysis of the cysteine string protein in Drosophila: cysteine string, linker and C terminus
... mutant protein was expressed in PC12 or HeLa cells, it was not palmitoylated and was not associated with membranes (Chamberlain and Burgoyne, ...the cysteine string is required for correct targeting of ...
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Thiol reactive reagents inhibits intracellular trafficking of human papillomavirus type 16 pseudovirions by binding to cysteine residues of major capsid protein L1
... because the incubation of the 16PVs with the thiol-reac- tive reagents induced a large effect on their infectivity. The 16PVs lost their infectivity after binding of the thiol-reac- tive reagents to the free thiols. The ...
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Role of Conserved Cysteine Residues in Hepatitis C Virus Glycoprotein E2 Folding and Function
... of cysteine pairs involved in di- sulfide ...functional protein fold, whereas simultaneous Ala substitutions rescued both folding and function in 2 of 10 disul- fides ...E2 protein associated with ...
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Sampling the Conformation of Protein Surface Residues for Flexible Protein Docking
... effective residues with flexible side-chains they ...effective residues would lead to a combinatorial explosion in the process of exploring their conformational ...that sizes of up to 9 ...
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Four Conserved Cysteine Residues of the Hepatitis B Virus Polymerase Are Critical for RNA Pregenome Encapsidation
... Pol protein plays multiple critical functions in viral assembly and replication and has been the focus of in- tense efforts to develop anti-HBV drugs ...soluble protein necessary to obtain a crystal ...
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Expression and Mutational Analysis of Autographa californica Nucleopolyhedrovirus HCF-1: Functional Requirements for Cysteine Residues
... the protein to punctate nuclear ...of cysteine residues within a putative RING finger domain in the amino acid sequence of HCF-1 abolished self-association activity and suggests that the RING domain ...
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Improved identification of wheat prolamins through alkylation of cysteine residues and peptide-based mass spectrometry
... for protein character- ization 3 ...gluten protein sequences results in a substantial sequence ...low cysteine levels in gluten proteins ...of cysteine-labeled gluten peptides, despite their ...
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