proteinase K-resistant prion protein
Distinct Proteinase K Resistant Prion Protein Fragment in Goats with No Signs of Disease in a Classical Scrapie Outbreak
... different prion strains in a given host ...multiple prion strains, which resulted in different disease phe- notypes, including distinct PrP res banding patterns (29, ...
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Accumulation of proteinase K-resistant prion protein (PrP) is restricted by the expression level of normal PrP in mice inoculated with a mouse-adapted strain of the Creutzfeldt-Jakob disease agent.
... electroporation. The cells were selected by both G418 (200 m g/ml) and FIAU (200 nM) for the first 4 days and by G418 alone thereafter. Drug-resistant colonies were transferred to 96-well dishes with feeder cells ...
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Prion infectivity is encoded exclusively within the structure of proteinase K-resistant fragments of synthetically generated recombinant PrPSc
... murine prion strain consists of at least 2 types of PK- sensitive PrP Sc : one is thermolysin-resistant and contributes little to prion infectivity, while the other is thermolysin- sensitive and ...
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Gauczynski, Sabine (2002): Characterization of the 37-kDa/67-kDa laminin receptor as the cell surface receptor for the cellular prion protein. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... to proteinase K (PK). Some mutant isoforms of the mouse prion protein carrying mutations homologous to familial human prion diseases were produced in stably transfected CHO cells and ...
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Transmissible Spongiform Encephalopathy and Cattle in Enugu State of Nigeria
... [8,20]. Prion ptotein is expressed predominantly in the brain, spinal cord and lymphoid tissues [13] and the result in Table 1 also agrees that the extraction buffer used was ...proeinase k used. PrP c is ...
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Cytosolic Prion Protein Toxicity Is Independent of Cellular Prion Protein Expression and Prion Propagation
... to proteinase K (PK) (21, ...initiate prion disease or act as a toxic element of ...a prion disease, as there are clear examples of human forms of prion disease that do not develop ...
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Proteinase K-Resistant Material in ARR/VRQ Sheep Brain Affected with Classical Scrapie Is Composed Mainly of VRQ Prion Protein
... 171R protein spot was observed, the amount of ARR material in the pI 8 spot approximated, on average, ...total protein spots at the glycosylated and nonglycosylated positions (ARR plus VRQ spots, following ...
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Rapidly progressive dementia with thalamic degeneration and peculiar cortical prion protein immunoreactivity, but absence of proteinase K resistant PrP: a new disease entity?
... precursor protein, APP (1:500, Millipore, Billerica, MA, ...pect prion disease but were found to have other disorders, and prion disease was formally ...
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Prions: in[PSI]t from Yeast
... the prion hypothesis and have even extended it to include a protein-only mechanism of inheritance in addition to the protein-only mechanism of infectivity seen in ...
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Topoisomerase I is preferentially associated with isolated replicating simian virus 40 molecules after treatment of infected cells with camptothecin.
... The 5' hydroxyls are associated with DNA molecules containing bound protein, since treatment of the Hirt supernatant with proteinase K prior to centrifugation in neutral CsCl greatly red[r] ...
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Prion diseases: immunotargets and therapy
... more research before they can be transferred into large-scale clinical trials. Further information is required to determine the most effective type of adaptive immune response to delay or even reverse disease ...
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Serological cross-reactions between Coxiella burnetii and Legionella micdadei.
... and protein immunoblot- ...with proteinase K indicated that cross-reactions were probably due to both protein and lipopolysaccharide ...
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Identification and characterization of a protein covalently bound to DNA of minute virus of mice.
... The labeling efficiency of T4 polynucleotide kinase did not improve when either the vDNA or the RF DNA was digested with either pronase or proteinase K, indicating that the protein was n[r] ...
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Processing of the Bovine Spongiform Encephalopathy-Specific Prion Protein by Dendritic Cells
... Prion infectivity in BMDC incubated with BSE prions. To quantify prion infectivity in DC cultures, a time course of in vitro infection was performed by in vivo titration of cell lysates (Table 1). After 1 ...
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Differences in Proteinase K Resistance and Neuronal Deposition of Abnormal Prion Proteins Characterize Bovine Spongiform Encephalopathy (BSE) and Scrapie Strains
... differences were found in long-term proteinase K resistance of PrPSc from the BSE-G isolate propagated in homozygous Prnpa C57BL/6 mice and Prnpb VM95 mice, PrPScs of scrapie strain. 22A[r] ...
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PubMedCentral-PMC4774245.pdf
... which prion disease is not closely surveilled and we cannot rule out a higher incidence than that reported in developed countries, yet the individuals with reportedly pathogenic variants in either ExAC or 23andMe ...
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Original Article Expression of prion protein in glioblastoma and related mechanisms
... targeted prion proteins (Forward, 5’-GGCAT AATCC AGGCA CCAAG GCAGG C-3’; Reverse, 5’-CAGCA CCATG CTCCA GGAAG GAGGC A-3’) and controlled siRNA (Forward, 5’-GTCCG CAGCA TGGCA CCAAG AAAGG C-3’; Reverse, CACTG AGCCT ...
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Investigation of serum protein profiles in scrapie infected sheep by means of SELDI TOF MS and multivariate data analysis
... any protein, re- gardless of specificity that significantly differs between scrapie affected and healthy controls, will contribute to novel information of underlying pathological processes of ...
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Prion Neurotoxicity: Insights from Prion Protein Mutants
... underlie prion-induced ...between prion infectivity and ...familial prion disorders, such as octapeptide insertions, small, B-rich aggregates are likely to represent a key neurotoxic ...
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Stimulation of TLR4 by recombinant HSP70 requires structural integrity of the HSP70 protein itself
... teinase K-agarose and control agarose an additional four ...nase K-agarose slurry on a shaking platform for 3 hr at ...from protein samples treated with control agarose slurry or Proteinase ...
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