Purification and characterisation of animal antimicrobial peptides
Expression, purification and characterisation of antimicrobial peptides of human and bovine lactoferrins : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Biochemistry at Massey University, Palmerston North, New Zealand
... in exerting its antimicrobial activity. This transition could be induced by the release of LFcin-B from bovine lactoferrin by pepsin cleavage. In the absence of proteolysis, it is possible that membrane ...
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The characterisation of ultrafiltration membranes used in water purification
... 1.1 The Need for Water Purification Inadequate access to sanitised and purified water is a pervasive problem that afflicts populations globally. As a result, millions are afflicted by preventable diseases, caused ...
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The identification and characterisation of antimicrobial peptides from snake and scorpion venom
... Peptides align with their hydrophobic side facing the lipids whilst the hydrophilic facet allows for further peptide aggregation through hydrogen bonding; lipids that are in contact with the hydrophilic face ...
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Characterisation of novel antimicrobial peptides from Egyptian scorpion and snake venoms
... Iron (Crosa, Walsh 2002, Dale, D oherty-K irby e t al. 2004). Also Fur has roles in cellular oxidative stress defence such as activation o f superoxide dism utase sodB w hich is involved in oxidative stress response ...
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The identification and characterisation of novel antimicrobial resistance genes from human and animal metagenomes
... Novel tetracycline resistance determinants have also been identified from the human oral cavity including a novel ABC transporter, TetAB(46) from Streptococcus australis and a tetracyc[r] ...
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The Antimicrobial Peptides: Ready for Clinical Trials?
... At first the peptides permeate and traverse the outer membrane and enter the periplasmic space. Then the process looks for the stereospecific and irreversible translocation into the cytoplasma of the bacterial ...
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Purification and partial characterization of antimicrobial peptides in the gill epidermal mucoprotein of the red banded grouper, epinephelus fasciatus, (forsskal, 1775)
... The peptide of grouper fish epinephenisin contains ß–sheet and alpha-helical structure with tyrosine as amino acids chain i.e. cationic residues, contributes additional positive charge to the mature peptide and enhances ...
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Characterisation of antimicrobial usage in cats and dogs attending UK primary care companion animal veterinary practices
... RVC OPEN ACCESS REPOSITORY – COPYRIGHT NOTICE This is the peer-reviewed, manuscript version of the following article: Buckland, E. L., O'Neill, D., Summers, J., Mateus, A., Church, D., Redmond, L. and Brodbelt, D. (2016) ...
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Development of an antimicrobial cement using human defensins-like antimicrobial peptides
... 42 From Table 3.12, it can be noticed that the observed molar mass of the product was 1447.8 g/mol and it matched the theoretical molar mass of 1448.6 g/mol (Table 3.1). Figure 3.12 shows the HPLC spectrum of AP4 after ...
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Development and optimization of topical antimicrobial agents based on human antimicrobial peptides
... bioactive peptides require low cost, effective methods for their produc- ...recombinant peptides in Escherichia coli very well suited for the production of toxic peptides like antimicrobial ...
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The cooperative behaviour of antimicrobial peptides in model membranes
... How peptides with antimicrobial actions kill the bacteria is subject to continuous ...of peptides can play a role in the bacterial membrane ...AFM characterisation Chen et al 15 suggested a ...
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CiteSeerX — Review Novel Formulations for Antimicrobial Peptides
... The efficacy of a 0.5% CsA micelle formulation was tested and compared to a physiological saline solution and to a systemic administration of CsA. The topical treatment for 14 days led to a significantly higher cornea ...
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Recombinant expression and activity of cationic antimicrobial peptides
... small antimicrobial peptides, was overexpressed in the soluble ...for purification and (2) to modify the surface of cotton fabrics with LL37, aiming at obtaining textiles with antibacterial and ...
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Biofilms: Novel strategies based on antimicrobial peptides
... To validate results in vitro, that could be further translated into higher organisms or clinical trials, it is necessary to use the right in vivo model [ 68 ]. It is very difficult to reproduce the host environment in in ...
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On the in vivo significance of bacterial resistance to antimicrobial peptides
... as animal models, offer several advantages, includ- ing the generally lower cost of non-primate animal studies compared with human studies, the ability to study later stages of infection, and the ability to ...
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FAST AND EFFICIENT PURIFICATION OF SYNTHETIC PEPTIDES BY SOLID-PHASE EXTRACTION
... Post-synthesis purification of peptides is often the most difficult part of the synthetic ...the purification of two synthetic antimicrobial peptides (calcitermin and temporin A) by ...
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Characterisation of three alpha helical antimicrobial peptides from the venom of Scorpio maurus palmatus
... Characterisation of three alpha-helical antimicrobial peptides from the venom of Scorpio maurus palmatus.. HARRISON, Patrick L., ABDEL-RAHMAN, Mohamed A., STRONG, Peter, TAWFIK, Mohame[r] ...
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Antimicrobial peptides in infected wounds
... Tel: +351-253-510-283; Fax: +351-253-510-293, Email: helena.felgueiras@2c2t.uminho.pt Abstract Wound healing is a fundamental process to re-establish tissue integrity. Microbial infections, however, may hinder this ...
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On the Physiology and Pathophysiology of Antimicrobial Peptides
... Republic Antimicrobial peptides (AMP) are a heterogeneous group of molecules involved in the nonspecific immune responses of a va- riety of organisms ranging from prokaryotes to mammals, including ...
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An Update on Antimicrobial Peptides (AMPs) and
... and in vivo Efficacy of the Antimicrobial Peptide DPK-060 Used for Topical Treatment. Vlieghe, P.; Lisowski, V.; Martinez, J.; Khrestchatisky, M[r] ...
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