Rainbow Trout Gill Cells
Immunolocalization of proton pumps (H+ ATPase) in pavement cells of rainbow trout gill
... the trout gill pavement cell is in stark contrast to other acid-excreting epithelia such as frog skin (Ehrenfeld et ...mitochondria-rich cells that are structurally analogous to the fish gill ...
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Interactions of highly and low virulent Flavobacterium columnare isolates with gill tissue in carp and rainbow trout
... and rainbow trout ( Oncorhynchus mykiss Walbaum) were ...and rainbow trout inoculated with the HV-isolate disclosed bacterial invasion and concomitant destruction of the gill tissue, ...
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Localization and characterization of phenamil sensitive Na+ influx in isolated rainbow trout gill epithelial cells
... the cells resuspended in 1–2 ml of anti-FITC microbeads (10 µl microbead stock per ml PBS; Mitylnei Biotech, Auburn, CA, USA) and incubated (20 min, 4°C with continuous ...the cells resuspended in 1 ml or 2 ...
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The cultured branchial epithelium of the rainbow trout as a model for diffusive fluxes of ammonia across the fish gill
... non-adherent cells and mucus, and new cells freshly isolated from a second fish were then seeded at the same density onto the adherent layer of cells established in the ...non-adherent cells ...
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Fooling a freshwater fish: how dietary salt transforms the rainbow trout gill into a seawater gill phenotype
... accessory cells and adjacent chloride cells will require further evaluation using higher resolution electron ...FW gill into the full SW ...1983), rainbow trout (Salman and Eddy, 1990) ...
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Branchial expression and localization of SLC9A2 and SLC9A3 sodium/hydrogen exchangers and their possible role in acid–base regulation in freshwater rainbow trout (Oncorhynchus mykiss)
... on gill tissue sections using the homologous NHE2 ...the trout NHE2 antibody was not ...same gill sections previously subjected to immunocytochemistry to identify PNA + and PNA – ...numerous ...
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The linkage between Na+ uptake and ammonia excretion in rainbow trout: kinetic analysis, the effects of (NH4)2SO4 and NH4HCO3 infusion and the influence of gill boundary layer pH
... pavement cells rather than chloride cells as the more likely site (Goss et ...pavement cells (increased density of mitochondria and apical microvilli) suggestive of increased transport activity have ...
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Responsiveness of gill Na+/K+ ATPase to cortisol is related to gill corticosteroid receptor concentration in juvenile rainbow trout
... thymoma-derived cells (Danielsen and Stallcup, 1984), but not in human leukocytes from leukaemia patients (Homo et ...between gill cytosolic CR B max and in ...
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Activation and nuclear translocation of ERK in response to ligand dependent and independent stimuli in liver and gill cells from rainbow trout
... a pH adjusted to 7.6 and an osmolarity of 284·mOsmol·l –1 . Hypo-osmotic conditions were created by mixing equal volumes of standard saline with the same medium lacking NaCl, yielding 0.58 ⫻ iso-osmolarity (165·mOsmol·l ...
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Reciprocal expression of gill Na+/K+ ATPaseα subunit isoforms α1a and α1b during seawater acclimation of three salmonid fishes that vary in their salinity tolerance
... the gill epithelium in the active ion uptake model of freshwater fishes (Avella and Bornancin, ...in rainbow trout (Reid et ...of gill Na + /K + -ATPase is therefore critical for ion ...
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Characterization of a branchial epithelial calcium channel (ECaC) in freshwater rainbow trout (Oncorhynchus mykiss)
... Homologous polyclonal antibodies raised against rtECaC were used in conjunction with a heterologous mouse monoclonal Na + /K + -ATPase antibody ( ␣ 5) to examine the cellular distribution of rtECaC in the gill ...
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IMMUNOLOCALIZATION OF H+ ATPase IN THE GILL EPITHELIA OF RAINBOW TROUT
... The localization of the proton pump in fish gills is not known. It is possible that the pump is situated in the apical membranes, as in frog skin, turtle bladder and mammalian kidney. Experimentally, drugs and treatments ...
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A Single Amino Acid Mutation (I1012F) of the RNA Polymerase of Marine Viral Hemorrhagic Septicemia Virus Changes In Vitro Virulence to Rainbow Trout Gill Epithelial Cells
... the ring domain of the polymerase (30, 31). It has been shown that point mutations (in the CRIV region) of Sendai virus and parain- fluenza 3 virus render these viruses temperature sensitive (32, 33). Further, mutation ...
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Physiological modulation of iron metabolism in rainbow trout (Oncorhynchus mykiss) fed low and high iron diets
... cells (63% NADH dependent; Riedel et al., 1995). We also incidentally noted an NADH-independent component in trout intestine (data not shown). A 5-fold higher net Fe retention in fish fed the low-Fe diet ...
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Cloning and molecular characterisation of the trout (Oncorhynchus mykiss) vacuolar H(+) ATPase B subunit
... information from cloned B subunits was used to design degenerate primers for semi-nested PCR of a trout gill/kidney cDNA library. An 810 base pair (bp) cDNA fragment was amplified and cloned to produce the ...
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An endogenous crepuscular rhythm of rainbow trout (Salmo gairdneri) photomechanical movements
... An endogenous crepuscular rhythm of rainbow trout 385 DISCUSSION The results show that rainbow trout possess an endogenous retinomotor rhythm during extended periods of darkness, with tw[r] ...
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Chromatic organization of cone photoreceptors in the retina of rainbow trout: single cones irreversibly switch from UV (SWS1) to blue (SWS2) light sensitive opsin during natural development
... In accordance with previous findings from other salmonid species (Novales Flamarique, 2005; Cheng et al., 2006; Cheng et al., 2007), this study shows that the vast majority of single cones express UV opsin mRNA in the ...
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Mitochondrial protein synthesis in rainbow trout (Oncorhynchus mykiss) heart is enhanced in sexually mature males but impaired by low temperature
... The impact of temperature on heart protein turnover is poorly understood. Protein synthesis was assessed in intact, perfused hearts isolated from rainbow trout. Acute changes in assay temperature between 5 ...
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Effects of sustained swimming on hepatic glucose production of rainbow trout
... resting state, and the effects of swimming on glucose fluxes are unknown. Maximal exercise and anaerobic metabolism have been the focus of most studies of carbohydrate utilization in teleosts (Moyes and West, 1995) even ...
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Sexual plasticity of ovarian germ cells in rainbow trout
... In this study, donor-derived oogonia, transplanted into male recipients, could produce fully functional sperm. Furthermore, during these studies we always obtained recipients showing a nearly 1:1 sex ratio and did not ...
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