RAW 264.7 macrophages
Acanthopanax koreanum roots inhibit the expression of pro-inflammatory cytokines, inducible nitric oxide synthase, and cyclooxygenase-2 in RAW 264.7 macrophages
... Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA). LPS (Escherichia coli 0111:B4) was purchased from Sigma Aldrich (St. Louis, MO, USA). All other ...
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Differences in anti inflammatory effect of immature and mature of Rubus coreanus fruits on LPS induced RAW 264 7 macrophages via NF κB signal pathways
... present study, IRF contained a higher quantity of EA than MRF did, indicating that the EA component in IRF inhib- ited the production of pro-inflammatory cytokines and inflammation-related factors in LPS-stimulated ...
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Inhibition of lipopolysaccharide (LPS) induced neuroinflammatory response by polysaccharide fractions of Khaya grandifoliola (C D C ) stem bark, Cryptolepis sanguinolenta (Lindl ) Schltr and Cymbopogon citratus Stapf leaves in raw 264 7 macrophages and U87 glioblastoma cells
... polysaccharides of these three plants have not yet been elucidated. This study aimed at investigating potential use of K. grandifoliola, C. sanguinolenta and C. citratus polysaccharides in the prevention of chronic ...
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Ethanol extract of Poria cocos reduces the production of inflammatory mediators by suppressing the NF kappaB signaling pathway in lipopolysaccharide stimulated RAW 264 7 macrophages
... activated macrophages during pathogen infection and they are involved in the up-regulation of in- flammatory ...in RAW 264.7 macrophages; while pre-treatment with EEPC significantly inhibited this ...
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Anti inflammatory effect and action mechanisms of traditional herbal formula Gamisoyo san in RAW 264 7 macrophages
... Although synthetic drugs have been used for treat- ment of inflammatory diseases such as atherosclerosis, many of them exhibit varying degrees of adverse ef- fects [45]. By contrast, an examination of herbal med- icines ...
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Herbal formula SC E1 suppresses lipopolysaccharide stimulated inflammatory responses through activation of Nrf2/HO 1 signaling pathway in RAW 264 7 macrophages
... LPS activates the receptors by binding Toll-like recep- tor 4 and triggers the MAPK pathway, a major intracel- lular signaling pathway, and once activated, this pathway leads to the activation of NF-κB [40]. Therefore, ...
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Synergistic interaction between C5a and NOD2 signaling in the regulation of chemokine expression in RAW 264 7 macrophages
... MDP-treated RAW 264.7 macrophages. RAW 264.7 macrophages were first pre- treated with MDP for 16 h and then media changed be- fore cells were treated or not with SB20358, to inhibit p38 ...
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YC-1 induces lipid droplet formation in RAW 264.7 macrophages
... Quantification of lipid storage compartments in macro- phages and ox-LDL-induced foam cells was performed, based on a previously-published protocol [18]. RAW 264.7 macrophages were seeded in a Costar® ...
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An ethyl acetate fraction derived from Houttuynia cordata extract inhibits the production of inflammatory markers by suppressing NF кB and MAPK activation in lipopolysaccharide stimulated RAW 264 7 macrophages
... Activation of NF-κB, an essential transcription factor in the inflammatory response, occurs following the phos- phorylation, ubiquitination, and proteolytic degradation of IκBα. Thus, p65 levels in cytoplasmic and ...
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Chungsimyeonja eum inhibits inflammatory responses in RAW 264 7 macrophages and HaCaT keratinocytes
... Keratinocytes produce various chemokines, which are involved in the development of inflammatory diseases. TARC and MDC are produced by dendritic cells, endothelial cell and keratinocytes which bind and attract CCR4+ Th2 ...
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The uptake of tocopherols by RAW 264.7 macrophages
... The uptake and depletion of alpha- and gamma-toco- pheryl quinone separately (A) and together (B) by RAW264.7 Macrophages. Confluent RAW264.7 macro- phages were incubated with either 0.6 m M alpha-tocopheryl ...
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Differential activation of RAW 264.7 macrophages by size-segregated crystalline silica
... macrophages. This study provided novel data showing that UF silica particles enhance the activation of AM when compared to larger silica particles usually represented in in vitro and in vivo research. These data ...
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Inhibition of lipopolysaccharide induced nitric oxide and prostaglandin E2 production by chloroform fraction of Cudrania tricuspidata in RAW 264 7 macrophages
... Cellular proteins were extracted from control and CT- treated RAW 264.7 cells. Briefly, cells were collected by centrifugation and the pellets so obtained were washed once with phosphate-buffered saline (PBS). ...
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Anti Inflammatory Effect of 3 Methylcarbazoles on RAW 264 7 Cells Stimulated with LPS, Polyinosinic Polycytidylic Acid and Pam3CSK
... activated RAW 264.7 macrophages induced by lipopolysaccharide (LPS), Poly(I:C), and pam3CSK was investigated by assessing nitric oxide (NO) and pro-inflammatory ...pam3CSK-activated macrophages but ...
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Molecular basis of the anti inflammatory potential of a diarylheptanoid in murine macrophage RAW 264 7 cells
... Alpinia officinarum is a traditional medicinal plant used for treating inflammatory and gastrointestinal dis- orders. It belongs to the Zingiberaceae family, which includes other important medicinal plants such as Cur- ...
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Babesia divergens glycosylphosphatidylinositols modulate blood coagulation and induce Th2 biased cytokine profiles in antigen presenting cells
... by RAW 264.7 macrophages (personal obser- ...10 7 tachyzoites to jump to about 2000 pg/mL with GPIs from 10 8 and 10 9 tachy- zoites, suggesting an all-or-nothing threshold to be passed for optimal ...
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Antioxidant activities and oxidative stress inhibitory effects of ethanol extracts from Cornus officinalis on raw 264 7 cells
... The mechanism of inflammation injury partially involves the release of ROS from activated neutrophils and macro- phages. ROS propagate inflammation by stimulating the release of cytokines such as interleukin-1, tumor ne- ...
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Anti inflammatory activities of Guang Pheretima extract in lipopolysaccharide stimulated RAW 264 7 murine macrophages
... The activity of NO synthesis can be deduced from the nitrite content of the culture medium as determined by the Griess reaction [29, 30]. RAW 264.7 cells were seeded in a 24-well plate at a density of 4 × 10 5 ...
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Specifically targeted delivery of protein to phagocytic macrophages
... Abstract: Macrophages play important roles in the pathogenesis of various diseases, and are important potential therapeutic ...Furthermore, macrophages are key antigen-presenting cells and important in ...
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Vaccaria hypaphorine alleviates lipopolysaccharide induced inflammation via inactivation of NFκB and ERK pathways in Raw 264 7 cells
... NO is identified to be a pro-inflammatory molecule in the development of various inflammatory diseases. The production of NO is widely taken as a hallmark for macrophage activation, which is indispensable for the ...
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