Real time polymerase chain reaction (real time PCR)
Detection of viable Salmonella in ice cream by TaqMan real-time polymerase chain reaction assay combining propidium monoazide
... Real-time polymerase chain reaction (PCR) allows rapid detection of Salmonella in frozen dairy products, but it might cause a false positive detection result because it might amplify ...
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One Step Real Time Polymerase Chain reaction using for detection of Plum Pox Potyvirus
... STEP REAL-TIME POLYMERASE CHAIN REACTION USING FOR THE DETECTION OF PLUM POX POTYVIRUS C VETANKA K ULUKOVSKA , E MILIJA A RSOV , S ASA M ITREV ...
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Development of Real-Time Polymerase Chain Reaction Assays for the Detection and Differentiation of Infectious Bursal Disease Virus Subtypes
... different real-time polymerase chain reaction (PCR) detection approaches based on SYBR Green I dye and Taqman probe based assays were developed for the detection and differentiation of ...
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Value of histopathology, immunohistochemistry, and real-time polymerase chain reaction in the confirmatory diagnosis of Encephalitozoon cuniculi infection in rabbits
... Miriam Leipig, 1 Kaspar Matiasek, Heinz Rinder, Dirk Janik, Daniela Emrich, Kerstin Baiker, Walter Hermanns Abstract. Morphological lesions in kidneys and brain are all too often considered diagnostic for confirmation of ...
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SHORT TECHNICAL REPORT for OsHV-1 detection and quantification by Real Time Polymerase Chain Reaction using OsHV-1 DNA polymerase sequence
... by Real Time Polymerase Chain Reaction using OsHV-1 DNA polymerase sequence” (available on the website of the EURL for mollusc ...
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Absolute quantitative real-time polymerase chain reaction for the measurement of human papillomavirus E7 mRNA in cervical cytobrush specimens
... SYBR-based real-time polymerase chain reaction (PCR) assays instead of performing Taqman-type assays allows low-cost, high-throughput analysis of viral mRNA ...
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Quantitative detection of Streptococcus mutans from saliva using FTATM elute cards and real time polymerase chain reaction
... Copyright © 2013 Sepideh Seghatoleslami et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, ...
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Performance evaluation of optimal real-time polymerase chain reaction achieved with reduced voltage
... Background: Polymerase chain reaction (PCR) is used in nucleic acid tests of infec‑ tious diseases in point‑of‑care ...demonstrated real‑time PCR that uses a micro‑PCR chip made of ...
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Development of real time polymerase chain reaction for detection of Salmonella typhimurium and Salmonella enteritidis in fish
... analysis time and reduces the power of PCR in terms of its rapid analysis when the result is needed immediately, especially during an ...the real time PCR performances, as well as c om par ing t he ...
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Rapid Detection of Shigella flexneri on Egg Samples by the Real Time Polymerase Chain Reaction Method
... Previous research by Mokhtari et al., (2013) stated that the use of the primer pair of ipaH genes has successfully detected the presence of Shigella species with the Real Time PCR method in food samples and ...
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Clustering techniques for DNA computing readout method based on real-time polymerase chain reaction
... and polymerase chain reaction ...TaqMan-based real-time PCR reactions are performed in parallel, to investigate the ordering of pairs of nodes in the Hamiltonian path, in terms of ...
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Real-Time Polymerase Chain Reaction for the Rapid Detection of Group B Streptococcal Colonization in Neonates
... by real-time PCR has also been reported in other ...infections, real-time PCR detected more patients than standard cultures, when discordant results were reconciled by a review of the ...
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Development of a fluorescent quantitative real time polymerase chain reaction assay for the detection of Goose parvovirus in vivo
... GPV detection by real-time PCR has only been reported by Bi [20]; in that study, the method was not optimized and a FQ-PCR standard curve was not generated. In this study, we reported the optimization of a ...
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Analyzing the gene expression profile of anaplastic histology Wilms’ tumor with real-time polymerase chain reaction arrays
... 88 real-time PCR primer pairs for quantitative gene expression analysis of key genes involved in pediatric anaplastic histology WT were designed and ...
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Urine real time polymerase chain reaction detection for children virus pneumonia with acute human cytomegalovirus infection
... that real-time PCR is more specific and sensitive than a chemilumines- cent assay for HCMV infection in 509 pediatric hospital- ized children with ...
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Ex vivo screening for immunodominant viral epitopes by quantitative real time polymerase chain reaction (qRT PCR)
... carcinoma [3]. Simian virus 40 (SV40) has been associ- ated with mesothelioma [4,5] and Human Papiloma Virus (HPV) with cervical cancer [6] and it appears that T cell reactivity may control their growth. Thus, we have ...
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Development of a new duplex real time polymerase chain reaction assay for hepatitis B viral DNA detection
... duplex real-time PCR assay is sufficiently sensitive, specific, accurate, reproducible and cost-effective for the detection of HBV ...duplex real-time PCR assay might be validated by larger ...
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Detection of notch1 c.7544_7545deICT mutation in chronic lymphocytic leukemia using conventional and real-time polymerase chain reaction
... to real-time ...reinvestigation. Real-time PCR, being less time-con- suming, showed advantage in the assessment of the amplification’s specificity (using the melting curve ...
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New Developments in Quantitative Real-time Polymerase Chain Reaction Technology
... The net effect of such interferences, at least in RT-qPCR, is the invariable quenching of the fluorescent signal, leading to sub-optimal or irreproducible gene expression data. Consequently, solutions are constantly ...
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Selection of suitable reference genes for quantitative real time polymerase chain reaction in human meningiomas and arachnoidea
... www.leonxie.com/referencegene.php. First according to the reference genes ranking by every algorithm from the most stable gene to the least stable gene, a series of con- tinuous integers starting from 1 as weight to each ...
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