Real-time RT-PCR
Real-time PCR and real-time RT-PCR applications in food labelling and gene expression studies
... to real-time PCR and later, real-time reverse transcription PCR (Real-Time ...conduct PCR detection of amplified gene products and expression analysis of ...
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Comparison of SYBR green I real time RT PCR with conventional agarose gel based RT PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco
... Tracheal samples were obtained from the Avian Unit, Agronomy and Veterinary Institute Hassan II, Rabat, Morocco. These samples were identified during rou- tine diagnostic efforts and stemmed from 34 commer- cial broiler ...
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Diagnosing rotavirus A associated IID: using ELISA to identify a cut off for real time RT PCR
... using real time RT-PCR, in 118 community IID cases and 65 healthy controls, previously tested by ...the real-time RT-PCR were used as a proxy measure of viral ...
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Real time RT PCR high resolution melting curve analysis and multiplex RT PCR to detect and differentiate grapevine leafroll associated virus 3 variant groups I, II, III and VI
... the RT-PCR HRM on a Qiagen Rotor-Gene Q thermal ...of PCR amplicons were obtained with temperatures ranging from 70°C to 90°C with a ...the RT-PCR HRM to differentiate between variants, ...
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Detection of a novel avian influenza A (H7N9) virus in humans by multiplex one step real time RT PCR assay
... a real- time RT-PCR assay, and highly virulent avian influenza A (H7N9) virus and other common respiratory pathogens were cultured in the State Key Laboratory for Diagnosis and Treatment of ...
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Evaluation of four commercial real time RT PCR kits for the detection of dengue viruses in clinical samples
... multiplex RT-PCR [6], has proved to be highly sensitive [7], and is used ...Conventional RT-PCR is being progressively replaced by real-time RT-PCR, which limits ...
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Development of one step SYBR Green real time RT PCR for quantifying bovine viral diarrhea virus type 1 and its comparison with conventional RT PCR
... RNA standards in this study were generated using BVDV RNA in cell cultures by in vitro transcription with T7 RNA polymerase for quantifying BVDV RNA by real-time RT-PCR. A RNA standard curve ...
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REAL TIME RT PCR ASSAY OF GENE FUMONISIN FUM5 RESPONSIBLE FOR MYCOTOXIN PRODUCTION ON RICE
... the Real-Time RT-PCR assay to quantify the expression of fum1 and fum19 genes from the Fumonisin-producing Fusarium ...its PCR based detection of Fusarium species was carried out by ...
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Simultaneous detection of novel H7N9 and other influenza A viruses in poultry by multiplex real time RT PCR
... Findings: In this study, a multiplex real-time RT-PCR (rRT-PCR) assay for rapid detection of H7N9 and other influenza A viruses was developed. To evaluate the assay, various influenza A ...
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One step real time RT PCR assays for serotyping dengue virus in clinical samples
... one-step real-time DENV RT-PCR [15] and the SD Bioline Dengue NS1 Ag Rapid Test (Stand- ard Diagnostics, ...DENV4 real-time RT-PCR assays with human ...
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Detection of Circulating Tumor Cells in Breast Cancer Patients Using Cytokeratin 19 Real Time RT PCR
... Methods: Real-time monitoring PCR of fluorescently labeled specific hybridization probes for CK-19 mRNA was es- ...the real-time RT-PCR ...
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Development of a duplex TaqMan real time RT PCR assay for simultaneous detection of newly emerged H5N6 influenza viruses
... duplex real-time RT- PCR assay was developed to identify H5N6 subtype ...hands-on time. Contrasting to single probe rRT- PCR, this method introduced two different labeled probes ...
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Development of a real time RT PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland
... by Real-time RT-PCR revealed that all 26 of the samples were genotype ...the RT-PCR ...the RT-PCR products to be applied directly to the membrane by allowing biotin ...
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A one step real time RT PCR assay for the quantitation of Wheat yellow mosaic virus(WYMV)
... croscopy, RT-PCR (reverse transcription polymerase chain reaction; [16] and RT-LAMP (reverse transcription loop- mediated isothermal amplification; [17] However, bio- logical assays are ...
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The development of a rapid SYBR one step real time RT PCR for detection of porcine reproductive and respiratory syndrome virus
... and time-consuming and thus is not suitable for rou- tine diagnostic ...conventional RT-PCR assays are labor intensive, as they require gel analysis for the PCR products, and they are not ...
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Asan qpcr Test COVID-19. SARS-CoV-2 Real-time RT-PCR Kit
... CDC 2019-n-CoV Real-Time RT-PCR Diagnostic Panel (CDC) N1 N2 N3 NewYorkDepartmentofHealth, SARS-CoV-2 Real-time ReverseTranscriptase(RT)-PCR Diagnostic Panel N N. Roche Molecular System [r] ...
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Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance
... one-step real-time RT-PCR assay for the detection of Middle East respiratory syndrome coronavirus ...reduced RT activity at low temperatures while achieving full activity at the ...
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Quantitative real-time RT-PCR of CD24 mRNA in the detection of prostate cancer
... of real-time RT-PCR for specific and sensitive detection of CD24 transcripts as a supplementary measure for discriminating between malignant and benign lesions in prostatic ...
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Superiority of Digital Reverse Transcription PCR (RT PCR) over Real Time RT PCR for Quantitation of Highly Divergent Human Rhinoviruses
... by RT-PCR is currently sufficient for deter- mining HRV infection, accurate quantification of HRV RNA in clinical samples is needed for studies associating HRV viral load with viral transmission and with ...
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The First Investigation of West Nile Virus in Horses Using Real Time RT-PCR in Middle Black Sea Region in Turkey
... by real time RT-PCR for WNV-RNA detection using the oligonucleotide primers and Taqman probe targeting WNV E gene designed by Lanciotti et ...OneStep RT-PCR Kit (Qiagen, Hilden, ...
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