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Recombinant protein secretion

Development of an improved Pseudoalteromonas haloplanktis TAC125 strain for recombinant protein secretion at low temperature

Development of an improved Pseudoalteromonas haloplanktis TAC125 strain for recombinant protein secretion at low temperature

... α-amylase secretion machinery in ...α-amylase-dependent recombinant secretion sys- ...the recombinant psychrophilic α-amy- lase as the wild type ...in recombinant protein ...

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Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion

Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion

... of secretion enhan- cing ...improve recombinant protein ...the secretion enhancing effect of AFT1 was confirmed in overexpressing strains, yielding up to ...

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Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris

Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris

... in protein sorting through the ER membrane may explain the beneficial effect of WSC4 overexpression on Fab ...soluble protein precursors, indicating defects in protein trafficking ...the ...

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Signal peptides for recombinant protein secretion in bacterial expression systems

Signal peptides for recombinant protein secretion in bacterial expression systems

... respective protein translocases in the cytoplasmic membrane ...mature protein part to allow its efficient interaction with posttranslationally interact- ing proteins (such as SecB) and, by this means, help ...

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A novel genetic system for recombinant protein secretion in the Antarctic Pseudoalteromonas haloplanktis TAC125

A novel genetic system for recombinant protein secretion in the Antarctic Pseudoalteromonas haloplanktis TAC125

... P. haloplanktis TAC125(pFCamy ∆ Ct-blaM) cells produced and secreted the Amy∆Ct-BlaM chimera as demonstrated by immunoblotting using anti-α-amylase antiserum (Fig- ure 4a). The chimera's linker was cleaved as occurred ...

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Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris

Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant proteins in Pichia pastoris

... trapped recombinant protein, we enforced the flux towards the secretory pathway, by overexpressing one of three ...import protein Sbh1, ER protein folding chaperone Kar2) and the late ...

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A propeptide toolbox for secretion optimization of Flavobacterium meningosepticum endopeptidase in Lactococcus lactis

A propeptide toolbox for secretion optimization of Flavobacterium meningosepticum endopeptidase in Lactococcus lactis

... highest secretion yields, although similar increases in secretion efficiencies were observed for PP3, PP2 and ...and secretion efficiency were obtained by ...heterologous protein expression ...

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Developing Gram-negative bacteria for the secretion of heterologous proteins

Developing Gram-negative bacteria for the secretion of heterologous proteins

... for recombinant protein production because they are fast growing, easy to manipulate, and genetically stable in large ...scales. Secretion of biotechnologically relevant proteins into the ...

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Quantification of the physiochemical constraints on the export of spider silk proteins by Salmonella type III secretion

Quantification of the physiochemical constraints on the export of spider silk proteins by Salmonella type III secretion

... of Secretion from the SPI-1 T3SS The psicA01 and psicA02 promoters enable inducible expression while maintaining the temporal dynamics encoded in the SPI-1 regulatory ...off, secretion could continue until ...

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Deciphering the Regulatory Network between the SREBP Pathway and Protein Secretion in Neurospora crassa

Deciphering the Regulatory Network between the SREBP Pathway and Protein Secretion in Neurospora crassa

... RNA-seq and data analysis. Mycelial biomass from 24-h VMM cultures previously transferred from a 16-h VMM culture was filtered and frozen in liquid nitrogen for RNA extraction. RNA-seq libraries were prepared in ...

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Interlaboratory Optimization and Evaluation of a Serological Assay for Diagnosis of Human Baylisascariasis

Interlaboratory Optimization and Evaluation of a Serological Assay for Diagnosis of Human Baylisascariasis

... Expression and purification of the rBpRAG1 antigen were verified by SDS-PAGE and immunoblotting. Nontransformed BL21(DE3)pLysS competent E. coli cells were used as controls to assess rBpRAG1 expres- sion. Aliquots of ...

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A Novel Mechanism for Protein Delivery by the Type 3 Secretion System for Extracellularly Secreted Proteins

A Novel Mechanism for Protein Delivery by the Type 3 Secretion System for Extracellularly Secreted Proteins

... FIG 4 A translocation motif inside the EspC Middle segment with homology to YopH is required for EspC translocation by the T3SS. (A) Homology of EspC and YopH in the internalization domain for the T3SS. Amino acid ...

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Modeling of protein secretion by cells

Modeling of protein secretion by cells

... Figure 2: Geometry in Comsol: Side view of a cell on a sensor, cut in half because of symmetry; cell in the lower left corner, sensor surface at the bottom, the measurements along the ho[r] ...

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S3440P Substitution in C-Terminal Region of Human Reelin Dramatically Impairs Secretion of Reelin from HEK 293T Cells

S3440P Substitution in C-Terminal Region of Human Reelin Dramatically Impairs Secretion of Reelin from HEK 293T Cells

... The secretion efficiency of recombinant wild-type and mutant Reelin constructs compared in cell lysate and supernatant isolated from the transiently transfected HEK 293T cells using 6XHistag ELISA ...

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The Ruler Protein EscP of the Enteropathogenic Escherichia coli Type III Secretion System Is Involved in Calcium Sensing and Secretion Hierarchy Regulation by Interacting with the Gatekeeper Protein SepL

The Ruler Protein EscP of the Enteropathogenic Escherichia coli Type III Secretion System Is Involved in Calcium Sensing and Secretion Hierarchy Regulation by Interacting with the Gatekeeper Protein SepL

... Active secretion, however, was detected only in the positive control, ...chimeric protein containing the initial 20-amino-acid N- terminal sequence of EscP fused to ...active secretion of full-length ...

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Production of a Candidate Recombinant Protein Vaccine for Mannheimia haemolytica in Lettuce and Tobacco Chloroplasts

Production of a Candidate Recombinant Protein Vaccine for Mannheimia haemolytica in Lettuce and Tobacco Chloroplasts

... (Figure 13A), termed pLSCTGG, contains upstream flanking sequences of 16S ribosomal rna (rrn16) then transfer RNA isoleucine (trnI) and downstream transfer RNA alanine (trnA) then 23S ribosomal rna (rrn23) to facilitate ...

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Pattis, Isabelle
  

(2007):


	Molekulare Mechanismen der Erkennung des CagA-Proteins als Substrat für das Cag-Typ IV-Sekretionssystem in Helicobacter pylori.


Dissertation, LMU München: Fakultät für Biologie

Pattis, Isabelle (2007): Molekulare Mechanismen der Erkennung des CagA-Proteins als Substrat für das Cag-Typ IV-Sekretionssystem in Helicobacter pylori. Dissertation, LMU München: Fakultät für Biologie

... das Protein Cagζ an den C-Terminus des green fluorescent protein ...bakterielles Protein einen toxischen Effekt auf eukaryotische Zellen zu haben ...

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A new potential secretion pathway for recombinant proteins in Bacillus subtilis

A new potential secretion pathway for recombinant proteins in Bacillus subtilis

... Thermostable β-galactosidase (BgaB) from Geobacillus stearothermophilus IAM11001 catalyses the hydrolysis of β-galactosides into monosaccharides and could be an ideal candidate for dairy processing [25], but it is diffi- ...

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Cloning, expression, and immunocharacterization of surface protein containing an altered thrombospondin repeat domain (SPATR) from Plasmodium knowlesi

Cloning, expression, and immunocharacterization of surface protein containing an altered thrombospondin repeat domain (SPATR) from Plasmodium knowlesi

... with recombinant pkSPATR protein in ELISA ...of recombinant pkHSPATR was differed between western blot and ELISA for detecting antibodies against ...the recombinant protein might ...

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High-level secretion of native recombinant human calreticulin in yeast

High-level secretion of native recombinant human calreticulin in yeast

... high-level secretion of recombinant human CRT in yeast culture media allowed one-step purification by Q-Sepharose ion-exchange ...acidic protein with a pI of ...CRT protein in the same single ...

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