recombinant His-tagged rat aminopeptidase B produced from baculovirus infected insect cells
Properties of Monoclonal Antibodies Directed against Hepatitis B Virus Polymerase Protein
... generated from a mouse immunized seven times over the time course of 1 ...MAbs from animals that had been immunized fewer times over shorter time periods were ...preparations from ...
9
The UL8 subunit of the herpes simplex virus helicase-primase complex is required for efficient primer utilization.
... We purified both the three-subunit helicase-primase complex and a UL5/UL52 subcomplex from recombinant baculovirus-infected insect cells and showed that the specific activities of the tw[r] ...
9
Novel Baculovirus Expression Vectors That Provide Sialylation of Recombinant Glycoproteins in Lepidopteran Insect Cells
... gene from AcDCW and effectively inactivates this parental viral ...isolate recombinant viruses with alterations in the gp64 region because homologous recombination with the transfer plasmid is required to ...
5
Bluetongue virus tubules made in insect cells by recombinant baculoviruses: expression of the NS1 gene of bluetongue virus serotype 10.
... To demonstrate that BTV-10 NS1 protein was synthesized in recombinant baculovirus-infected cells, protein extracts were prepared from insect cells infected with the recombinant virus AcB[r] ...
9
Recombinant virus vaccine for bluetongue disease in sheep.
... Bluetongue virus tubules made in insect cells by recombinant baculovirus: expression of NS1 gene of bluetongue virus serotype 10.. Structure of the bluetongue virus capsid.[r] ...
6
Molecular Cloning of a Babesia caballi Gene Encoding the 134-Kilodalton Protein and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay
... of B. caballi-infected erythrocytes (lane a) and not in the lysates of ...(lanes b and ...the recombinant BC134 protein, positive reactions were observed in all of the developmental stages of ...
5
Characterization and purification of human fos protein generated in insect cells with a baculoviral expression vector.
... To characterize fos protein produced by baculoviral vectors, insect cells were infected for various times with purified recombinant viruses and labeled with [35S]methionine for 3 h.. Tot[r] ...
10
Assembly of double-shelled, viruslike particles of bluetongue virus by the simultaneous expression of four structural proteins.
... Coexpression of the two major core proteins VP3 and VP7 in insect cells by a multiple recombinant baculovirus results in the synthesis of CLPs of the same size, appearance, and stoichiom[r] ...
6
Expression and self-assembly of empty virus-like particles of hepatitis E virus.
... of recombinant baculoviruses and expression of capsid pro- teins. Sf9 cells (Riken Cell Bank, Tsukuba, Japan) derived from an insect, Spodoptera frugiperda (27), were cotransfected with ...
7
Optimization of recombinant human transferrin expression in insect cells baculovirus system
... of recombinant glycoprotein, it is important that the recombinant protein can be generated in large ...the insect cells and baculovirus behaviour is as critical as the expressional ...
45
Baculovirus coinfection strategy for improved galactosylation of recombinant glycoprotein produced by insect cell culture
... To ensure successful galactosylation, cooperation of the substrate acceptor, substrate donor and enzyme is needed. The relationships among the three main elements of the in vivo galactosylation process, which were hTf as ...
6
High-Level Expression of Truncated Surface Antigen P50 of Babesia gibsoni in Insect Cells by Baculovirus and Evaluation of Its Immunogenicity and Antigenicity
... sera from dogs experimentally infected with ...proteins from insect cells or ...of insect cells infected with recombinant baculoviruses and, therefore, ...
6
Baculovirus Infection of Nondividing Mammalian Cells: Mechanisms of Entry and Nuclear Transport of Capsids
... The study of host-virus interactions not only contributes to our basic knowledge of virology and cell biology but also is important in the further development of gene therapy vector systems (31). We (35) and others ...
10
Assembly of viruslike particles by recombinant structural proteins of adeno-associated virus type 2 in insect cells.
... Preparations of VLPs from insect cells infected with different combinations of recombinant baculoviruses showed different relative proportions of the expressed proteins.. In addition, th[r] ...
9
Expression of L protein of vesicular stomatitis virus Indiana serotype from recombinant baculovirus in insect cells: requirement of a host factor(s) for its biological activity in vitro.
... COS cells, and the L protein effectively complemented a tempera- ture-sensitive mutant of VSV defective in L protein function ...the recombinant L protein transiently ex- pressed in COS cells has ...
8
Characterization of Toxoplasma gondii SAG2 Expressed in Insect Cells by Recombinant Baculovirus and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay
... a recombinant baculovirus carrying the gene encoding SAG2 of ...Sf9 cells do not recognize the GPI attachment site and degraded the full-length molecule ...
5
Evaluation of an Enzyme Linked Immunosorbent Assay with Recombinant Rhoptry Associated Protein 1 Antigen against Babesia bovis for the Detection of Specific Antibodies in Cattle
... immunogenic B-cell and T-cell epitopes on RAP-1 are conserved among all strains tested, but they are not conserved between different species (5, ...encoding B. bovis RAP-1 was expressed in insect ...
5
Identification of bluetongue virus VP6 protein as a nucleic acid-binding protein and the localization of VP6 in virus-infected vertebrate cells.
... frugiperda cells infected with the recombinant baculovirus or wild-type Autographa californica nuclear polyhedrosis virus AcNPV, or purified BTV-10 cores prepared from BHK-21 cells were [r] ...
8
Expression of Recombinant Small Hydrophobic Protein for Serospecific Detection of Avian Pneumovirus Subgroup C
... of baculovirus-produced hMPV N protein by Western blot analysis (unpublished ...with recombinant hMPV N protein also showed a positive reaction with APV/C-specific antibodies (data not ...
5
Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1.
... endowed infected cells with resistance to lysis by complement and antibody against glycoprotein B ...(gB). Cells infected with this mutant at the nonpermissive tempera- ture made normal ...
9