Renilla luciferase
Original Article Detection of HER2 positive circulating tumor cells using anti-HER2-renilla luciferase fusion protein
... The Renilla luciferase gene was cut with restriction enzymes (NheI/XbaI) from the pRL-TK vector (Promega Corporation, Madison, WI, USA) and then subcloned into a pcDNA3 expression ...
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Mutational Analysis of the Varicella-Zoster Virus ORF62/63 Intergenic Region
... each luciferase assay was based on the viral titer (36). The luciferase assay was performed with a dual-luciferase reporter assay system (Promega, Madison, WI), using cells or tissues harvested in ...
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Tombusvirus P19 RNA silencing suppressor (RSS) activity in mammalian cells correlates with charged amino acids that contribute to direct RNA-binding
... Firefly luciferase (Fluc), a Renilla luciferase (Rluc), and a shRNA (small hairpin RNA) targeting Firefly luciferase mRNA (sh-Fluc) ...
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Epstein-Barr Virus (EBV) Tegument Protein BGLF2 Promotes EBV Reactivation through Activation of the p38 Mitogen-Activated Protein Kinase
... AP-1–firefly luciferase reporter plasmid (A) or NF- B–firefly luciferase reporter plasmid (B), along with a Renilla luciferase plasmid and plasmids expressing EBV proteins, the VZV ORF12 ...
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<p>miR-139-5p functions as a tumor suppressor in cervical cancer by targeting TCF4 and inhibiting Wnt/β-catenin signaling</p>
... firefly luciferase plasmid, renilla luciferase plasmid, miR-139-5p mimics (Mimics) or miR-139-5p mimics plus TCF4 expression plasmids (Mimics+TCF4), TCF4 expression plasmids (TCF4) or controls ...
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Mumps Virus Nucleoprotein Enhances Phosphorylation of the Phosphoprotein by Polo-Like Kinase 1
... ponents. The minigenome system has been used to examine MuV RNA synthesis (7). Plasmids encoding P, L, and NP were trans- fected along with a plasmid carrying the MuV minigenome under a T7 promoter. When the minigenome ...
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Divergent MicroRNA Targetomes of Closely Related Circulating Strains of a Polyomavirus
... luciferase reporter upstream of a single copy of an approximately 1-kb fragment of the 3= UTR of ARCN1 or C9orf140 (wild type ...firefly luciferase expression vector individually into 293T cells, and ...
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Suppressor of Cytokine Signaling 3 Suppresses Hepatitis C Virus Replication in an mTOR-Dependent Manner
... What about the reciprocal effect of HCV on SOCS3 lev- els? We consistently observed a decrease of SOCS3 protein levels with prolonged HCV infection in multiple model sys- tems. The apparent dissociation of protein levels ...
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Class III Phosphatidylinositol 4-Kinase Alpha and Beta Are Novel Host Factor Regulators of Hepatitis C Virus Replication
... for luciferase activity at 72 h posttransfection by using a Bright-Glo luciferase assay ...and Renilla luciferase duplexes were purchased from Dharmacon (pGL2, catalog ...D-001140-01-20; ...
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NFAT and CREB Regulate Kaposi's Sarcoma-Associated Herpesvirus-Induced Cyclooxygenase 2 (COX-2)
... or various COX-2 promoter deletion constructs (P2-1900-luc, P2-1102-luc, P2-431-luc, P2-274-luc, P2-192-luc, and P2-150-luc). At 24 h after transfection, cells were serum starved and then left uninfected or infected with ...
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Loss of INPP4B causes a DNA repair defect through loss of BRCA1, ATM and ATR and can be targeted with PARP inhibitor treatment
... INPP4B loss results in a DNA repair defect Based on our findings that loss of INPP4B resembles features of BRCA1-negative tumors, we hypothesized that INPP4B deficiency may lead to defects in DNA repair. Stable knockdown ...
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Insights into Polyomaviridae MicroRNA Function Derived from Study of the Bandicoot Papillomatosis Carcinomatosis Viruses
... of Renilla luciferase (34), via the XhoI/XbaI ...The luciferase readings were col- lected using a Luminoskan Ascent microplate luminometer (Thermo Fisher ...miRNA Renilla luciferase ...
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Applications of bioluminescence imaging to the study of infectious diseases
... Bioluminescence imaging typically has been used to study infection in mouse models, but this imaging modal- ity can be used effectively in other organisms. For example, Harmache et al. used BLI to investigate infec- tion ...
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Optimization of Feline Immunodeficiency Virus Vectors for RNA Interference
... of Renilla luciferase (5⬘-GGCCTTTCACTACTCCTAC-3⬘) and was kindly pro- vided by Promega Technical ...firefly luciferase used in the 3 ⬘ Lucfrag vector was cloned by PCR using Taq polymerase (Biolase) ...
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BGLF2 Increases Infectivity of Epstein-Barr Virus by Activating AP-1 upon De Novo Infection
... firefly luciferase reporter and Renilla luciferase (internal control) ...factor- luciferase [TCF/LEF-luc] and heat shock element-luciferase [HSE-luc]), although the activations were ...
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A redesigned CRISPR/Cas9 system for marker free genome editing in Plasmodium falciparum
... ORF, renilla luciferase ORF and Plasmodium berghei dihydrofolate reductase (Pbdhfr; [PlasmoDB: PBANKA_0719300]) terminator (PbDT) were amplified from pLN-GFP (Additional file 2), pHBRIH and the genomic DNA ...
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Luminescent multiplex viability assay for Trypanosoma brucei gambiense
... of Renilla luciferase parasites has been ...vitro Renilla luciferase activity, can be combined with the CellTiter-Glo assay as an efficient lumi- nescent multiplex viability assay for HTS ...
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Mapping of a Region of the PA-X Protein of Influenza A Virus That Is Important for Its Shutoff Activity
... cells, Renilla luciferase was coexpressed without (mock) or with wild-type PA-X, PA_252, PA_252_X20, PA-X_6E, or ...PA-X_6E_X20. Renilla luciferase activity (B) and the expression of ...
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A regulatable transgene expression system for cultured Plasmodium falciparum parasites
... of Renilla luciferase to investigate its putative biological ...of Renilla luciferase and bsd were compared to the expression levels of several endogenous ...and Renilla ...
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Noncoding Flavivirus RNA Displays RNA Interference Suppressor Activity in Insect and Mammalian Cells
... Firefly luciferase (Fluc), Renilla luciferase (Rluc), and MBP or WNV sfRNA ...The luciferase activity was measured at 48 hpt, and the normalized relative luciferase activity (Fluc/Rluc) ...
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