Restriction Fragment Length Polymorphism Genotyping Methods
Sensitive PCR Restriction Fragment Length Polymorphism Assay for Detection and Genotyping of Giardia duodenalis in Human Feces
... a fragment of the tpi gene of ...gene fragment was amplified from 91 and 59% of the DNA samples extracted from whole feces and stained smears, respectively, in which the presence of Giardia cysts had been ...
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Genotyping Neisseria gonorrhoeaeUsing Fluorescent Amplified Fragment Length Polymorphism Analysis
... typing methods meet the need for higher resolution (3, 5, 10, 13, 14, 16, 18, 19, ...as restriction fragment patterns that are difficult to analyze objectively and are not easily stored in a ...
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Improved Genotyping Vaccine and Wild Type Poliovirus Strains by Restriction Fragment Length Polymorphism Analysis: Clinical Diagnostic Implications
... four restriction enzymes, HaeIII, DdeI, NcoI and AvaI, was sufficient for complete inter- and intratypic differentiation of ...four restriction endonucleases was ...provisional genotyping powers of ...
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Evaluation of PCR Restriction Profile Analysis and IS2404 Restriction Fragment Length Polymorphism and Amplified Fragment Length Polymorphism Fingerprinting for Identification and Typing of Mycobacterium ulcerans andM marinum
... PCR restriction profile analysis (PRPA), IS2404 restriction fragment length polymorphism (RFLP), and amplified fragment length polymorphism (AFLP) on a set of ...
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Restriction fragment length polymorphism analysis of clinical isolates of Mycobacterium haemophilum
... The identical RFLP patterns observed among different isolates that were collected over many years (16 years in the case of the type 2 clone) and from the same patient (patient 12) over 3[r] ...
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Generation of a restriction fragment length polymorphism linkage map for Toxoplasma gondii.
... of markers on chromosome VI likely underestimates its true genetic size. T h e further mapping of addi- tional DNA markers and mutant phenotypes will be useful for[r] ...
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Comparison of Two Target Genes for Detection and Genotyping of Giardia lamblia in Human Feces by PCR and PCR Restriction Fragment Length Polymorphism
... Received 16 June 2005/Returned for modification 2 August 2005/Accepted 28 September 2005 A PCR assay targeting the tpi gene was developed to detect and to genotype Giardia lamblia in human feces. Our assay was specific ...
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Differentiation of Mycobacterium tuberculosis isolates by spoligotyping and IS6110 restriction fragment length polymorphism
... London W2 1PG, and Hepatitis and Retrovirus Laboratory, Central Public Health Laboratory Service, Colindale, 2 United Kingdom, and Institute of Public Health, Bilthoven, The Netherlands 3 Received 9 September ...
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Restriction Fragment Length Polymorphism Analysis of Some Flagellin Genes of Salmonella enterica
... to restriction fragment length polymorphism (RFLP) analysis by using endonucleases HhaI and ...different restriction profiles, respectively, among 329 flagellin genes coding for 26 ...
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Species Identification of Mycobacteria by PCR Restriction Fragment Length Polymorphism of therpoB Gene
... PCR-restriction fragment length polymorphism analysis (PRA) using the novel region of the rpoB gene was developed for rapid and precise identification of mycobacteria to the species ...with ...
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Restriction fragment length polymorphism of the C1 inhibitor gene in hereditary angioneurotic edema
... Southern blot analysis of genomic DNA was performed after digestion with six different restriction endonucleases in 24 families affected with type 1 HANE (low antigenic and functional C1[r] ...
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Mapping of the X linked agammaglobulinemia locus by use of restriction fragment length polymorphism
... Significant linkage was detected between XLA and loci defined by two polymorphic DNA probes called 19-2 for the DXS3 locus and S21 for the DXS17 locus.. Tight linkage between XLA and the[r] ...
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Capillary Electrophoretic Restriction Fragment Length Polymorphism Patterns for the Mycobacterial hsp65 Gene
... one restriction enzyme, HaeIII, we investigated a total of 52 reference and clinical strains encompassing 12 Mycobacterium ...of restriction fragments resolved by CE, and the real sizes were deduced from ...
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Genetic Identification of the Main Opportunistic Mucorales by PCR Restriction Fragment Length Polymorphism
... Identification of clinical isolates by PCR-RFLP. An 830-bp band corresponding to the presence of Mucorales was ampli- fied from clinical isolates 1 and 2. In case 1, digestions were performed by using enzymes PpuMI, ...
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Identification of Infectious Agents in Onychomycoses by PCR Terminal Restriction Fragment Length Polymorphism
... PCR-terminal restriction fragment length polymorphism (TRFLP) assay to directly and routinely identify the infecting fungi in ...specific restriction fragment size of ...
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Restriction fragment length polymorphism of the periplasmic flagellar flaA1 gene of Serpulina species.
... LAURIE N. FISHER,† MICHELLE R. MATHIESEN, AND GERALD E. DUHAMEL* Department of Veterinary and Biomedical Sciences, University of Nebraska—Lincoln, Lincoln, Nebraska 68583-0905 Received 31 March 1997/Returned for ...
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TRAMPR: A package for analysis of Terminal-Restriction Fragment Length Polymorphism (TRFLP) data
... 1 Introduction TRAMPR is an R package for matching terminal restriction fragment length polymorphism (TRFLP) profiles between unknown samples and a database of knowns. TRAMPR facilitates ...
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Characterization of the Mycobacterium bovis Restriction Fragment Length Polymorphism DNA Probe pUCD and Performance Comparison with Standard Methods
... bovis restriction fragment length polymorphism (RFLP) typing probe pUCD was characterized by sequence analysis and the previously observed polymorphic banding pattern was reproduced with a ...
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Genotyping of Madurella mycetomatis by Selective Amplification of Restriction Fragments (Amplified Fragment Length Polymorphism) and Subtype Correlation with Geographical Origin and Lesion Size
... Institute of Endemic Diseases, and Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan 4 Received 11 February 2005/Returned for modification 31 March 2005/Accepted 2 June 2005 One of the ...
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RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF RHIZOBIUM SP ISOLATED FROM DOLICHOUS LABLAB
... Dolichous lablab is known for its dietary protein source, medicinal properties and symbiotic nitrogen fixation by Rhizobium present in its root nodules. Rhizobium sp. was identified by biochemical methods from ...
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