retroviral RNA
The retroviral RNA dimer linkage: different structures may reflect different roles
... of retroviral RNA dimer sequences in the context of animal models, the non-human retroviruses, including the non-primate lentiviruses will be of great ...up, retroviral dimeric genomes are linked by ...
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New windows into retroviral RNA structures
... the RNA, BzCn will be the most informative reagent, when used to perform a series of timed snapshots of the RNA ...of retroviral RNA structure, but also to verify that experimental conditions ...
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Analysis of the Contribution of Reverse Transcriptase and Integrase Proteins to Retroviral RNA Dimer Conformation
... genomic RNA that are linked noncovalently. The dimeric RNA of human immunodeficiency virus type 1 (HIV-1) undergoes rearrangement during virion maturation, whereby the dimeric RNA genome assumes a ...
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Destiny of Unspliced Retroviral RNA: Ribosome and/or Virion?
... genome-length retroviral RNA is utilized for both translation and ...of RNA viruses that utilize virally encoded reverse transcriptase (RT) to replicate genomic RNA through a proviral DNA in- ...
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The Gag Domains Required for Avian Retroviral RNA Encapsidation Determined by Using Two Independent Assays
... specific RNA packaging by 6- to 10-fold, consistent with previous observations that the NC Cys-His motifs played a role in assembly and RNA ...made, RNA packaging was reduced by more than 25-fold ...
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Specificity of retroviral RNA packaging.
... Thus, we have examined RNA encapsidation into virions produced by other avian and murine retroviral packaging cell lines in the presence and absence of RNAs containing defined retroviral[r] ...
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Monitoring Retroviral RNA Dimerization In Vivo via Hammerhead Ribozyme Cleavage
... target retroviral vectors were transfected and/or cotransfected into the murine retroviral PA317 (ATCC) pack- aging cell line to take advantage of constitutively produced viral proteins that enhance the ...
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An Immature Retroviral RNA Genome Resembles a Kinetically Trapped Intermediate State
... viral RNA, including packaging into virus particles and expression of viral genes, have only a small dependence on the structure or presence of PAL2 under conditions of replication in cell ...
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The evolution of novel fungal genes from non-retroviral RNA viruses
... Little is known of the biology of non-retroviral integrated RNA viruses (NIRVs). We are unaware, for example, of evolutionary or functional comparisons among NIRVs. Even the initial bioinformatic evidence ...
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cis-Acting Elements Important for Retroviral RNA Packaging Specificity
... virion RNA, whereas the other portion was used to infect D17 target ...virion RNA, total cellular RNA was isolated from each pool to directly examine the level of viral RNA expres- ...of ...
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A retroviral RNA secondary structure required for efficient initiation of reverse transcription.
... Second, U5 deletion and leader substitution mutations which would disrupt the U5-leader stem structure in ATVA6, ATVA15, ATVA21, and ATVAL resulted in a decrease in the efficiency of vir[r] ...
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Avian retroviral RNA encapsidation: reexamination of functional 5' RNA sequences and the role of nucleocapsid Cys-His motifs.
... We show that the functional ALV 5' 4 sequence is 270 nt or less in length, that spliced RNA can be encapsidated efficiently into retroviral particles, and that NC CysHis boxes are not re[r] ...
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One retroviral RNA is sufficient for synthesis of viral DNA.
... DISCUSSION In this study, we used previously characterized SNV-based retroviral vectors and helper cells to study the strand transfers that occur during the reverse-transcription phase o[r] ...
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Avian retroviral RNA element promotes unspliced RNA accumulation in the cytoplasm.
... for RNA packaging ...virion RNA in the absence of either DR ...viral RNA, which is necessary both for synthesis of virion proteins and for packaging of viral ...
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Modification of retroviral RNA by double-stranded RNA adenosine deaminase.
... products were cloned into pCRII by the TA cloning kit and sequenced with a Sequenase kit (U.S. Biochemical). Several PCR clones from each amplification reaction were sequenced. Five of six single-cell clones containing ...
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In vitro synthesis of infectious retroviral RNA.
... According to the favored model for retrovirus RNA transcription 13, the RNA synthesized in vitro from N2script should produce a normal DNA provirus after in vivo reverse transcription de[r] ...
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Moloney Murine Sarcoma Virus Genomic RNAs Dimerize via a Two-Step Process: a Concentration-Dependent Kissing-Loop Interaction Is Driven by Initial Contact between Consecutive Guanines
... Retroviruses contain two plus-strand genomic RNAs, which are stably but noncovalently joined in their 5 ⴕ regions by a dimer linkage structure (DLS). Two models have been put forward to explain the mechanisms by which ...
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Mechanism of retroviral genomic RNA dimerization and packaging
... vitro RNA dimerization reveals the structure of the mature ...longer retroviral RNA fragments require either the presence of the nucleocapsid protein or unphysiological temperatures (60 ...the ...
126
The matrix domain contributes to the nucleic acid chaperone activity of HIV 2 Gag
... HIV-1 RNA fragments and binds non-Ψ RNA with low specificity via its NC and MA domains, whereas bind- ing to Ψ RNA is highly specific and only the NC domain is engaged ...all retroviral Gag ...
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Genetic rearrangements occurring during a single cycle of murine leukemia virus vector replication: characterization and implications.
... Generation of packaging cell clones harboring a single parental vector pro- virus. Packaging cell clones containing a single parental provirus were produced by (i) the transfection of GP1E-86 cells with vector plasmid ...
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