Retroviral transduction
Adeno-associated virus type 2-mediated transfer of ecotropic retrovirus receptor cDNA allows ecotropic retroviral transduction of established and primary human cells.
... ral transduction efficiency, presumably because of efficient transduction and expression of the ecoR gene mediated by the recombinant ...of retroviral transduc- tion is generally less than 50% among ...
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Nuclear Import of Moloney Murine Leukemia Virus DNA Mediated by Adenovirus Preterminal Protein Is Not Sufficient for Efficient Retroviral Transduction in Nondividing Cells
... for retroviral transduction ...for retroviral integration, another cell culture system was used as a model to test the pTP-mediated retroviral transduction of nondividing ...for ...
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Approaches to enhancing the retroviral transduction of human synoviocytes
... flow-through transduction support those of Chuck and Palsson ...where transduction was already higher under static condi- tions, but increases of up to 3-fold were ...
5
Effective suppression of Dengue fever virus in mosquito cell cultures using retroviral transduction of hammerhead ribozymes targeting the viral genome
... tail that promotes hRz accessibility. A hygromycin resist- ance gene under control of a Rous Sarcoma Virus (RSV) promoter permits selection of transfected cells. The back- bone was derived from pQCXIH retroviral ...
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Arylsulfatase B activities and glycosaminoglycan levels in retrovirally transduced mucopolysaccharidosis type VI cells Prospects for gene therapy
... Rat chondrocyte cultures and retroviral transduction. Chondrocyte cultures were established from normal and MPS VI rats according to the method of Tsukazaki et al. (25), with several minor modifications. ...
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Molecular analysis and gene therapy of X-linked severe combined immunodeficiency
... any retroviral transduction system , the transduction protocol must be capable o f restoring T-lym phopoiesis and N K cell developm ent in vitro and in ...the transduction o f X SCID haem ...
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Transduction of helper T cells as a genetic approach to study mechanisms controlling their differentiation and function
... MIG retroviral system that gives good virus production is ...With retroviral transduction, several alternatives within these protocols have been ...the retroviral genes (for example reference ...
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Cationic Liposomes Enhance the Rate of Transduction by a Recombinant Retroviral Vector In Vitro and In Vivo
... Polybrene-enhanced transduction in the presence of 40 mg of heparan sulfate per ml; however, this had no effect on DC-Chol/DOPE-mediated enhancement (data not shown), suggesting that the mechanism of action of ...
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SOX9 transduction of a human chondrocytic cell line identifies novel genes regulated in primary human chondrocytes and in osteoarthritis
... that retroviral transduction with SOX9 of passaged human OA chondrocytes re-activated their potential to form cartilage matrix [15], we investigated the expression of the novel genes identified in SW1353 ...
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Moloney murine leukemia virus-derived retroviral vectors decay intracellularly with a half-life in the range of 5.5 to 7.5 hours.
... of retroviral transduction along with cell cycle kinetics was used to interpret these experimental results ...of retroviral DNA into the genome of the target ...
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Prevention of type 1 diabetes by gene therapy
... We hypothesized that if expression of diabetes-resistant MHC class II alleles on bone marrow–derived cells is sufficient to pre- vent diabetes, genetic engineering of autologous hematopoietic stem cells could be used to ...
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SOX9 transduction of a human chondrocytic cell line identifies novel genes regulated in primary human chondrocytes and in osteoarthritis
... that retroviral transduction with SOX9 of passaged human OA chondrocytes re-activated their potential to form cartilage matrix [15], we investigated the expression of the novel genes identified in SW1353 ...
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Multiple modifications in cis elements of the long terminal repeat of retroviral vectors lead to increased expression and decreased DNA methylation in embryonic carcinoma cells.
... produce retroviral vectors which overcome the inherent inactivity of the MoMuLV transcrip- tional unit in embryonic ...the retroviral LTR only for producing full-length viral genome in the packaging cell ...
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Monitoring Retroviral RNA Dimerization In Vivo via Hammerhead Ribozyme Cleavage
... Extracted retroviral genomic RNAs of various origins have been shown by electron microscopy to be linked close to their 59 ends in a parallel orientation, forming dimers (5, 15, 18); the dimerized region maps ...
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IFITM3 Reduces Retroviral Envelope Abundance and Function and Is Counteracted by glycoGag
... several retroviral and nonretroviral glycoproteins, revealing that this function is not specific to HIV-1 Env and thus unlikely to result from a specific IFITM3-Env ...
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Retroviral proteases
... The relationships between retroviral proteases. Protease sequences from several different immunodeficiency viruses are compared with endogenous retroviral sequences found in various eukaryotic genomes. The ...
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Specificity of retroviral RNA packaging.
... Thus, we have examined RNA encapsidation into virions produced by other avian and murine retroviral packaging cell lines in the presence and absence of RNAs containing defined retroviral[r] ...
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Use of different but overlapping determinants in a retrovirus receptor accounts for non reciprocal interference between xenotropic and polytropic murine leukemia viruses
... the retroviral envelope (Env) protein to specific cell-surface protein ...of retroviral infection, and occurs when newly-synthesized Env binds to receptor proteins resulting in a block to entry by ...
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Signal Transduction and Transcription Factor Modification during Reactivation of Epstein-Barr Virus from Latency
... signal transduction path- ways which have been established from the BCR in response to cross-linking with anti-Ig (reviewed in reference 7) are illus- trated in ...
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Amplification and chromosomal dispersion of human endogenous retroviral sequences.
... The initial results suggest that, like some of the class of truncated endogenous retroviral segments, some members of the full-length human retroviral family were also amplified and phys[r] ...
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