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Reverse Phase Protein Array

Assessment of a panel of tumor markers for the differential diagnosis of benign and malignant effusions by well-based reverse phase protein array

Assessment of a panel of tumor markers for the differential diagnosis of benign and malignant effusions by well-based reverse phase protein array

... and reverse-phase protein array ...one protein, it is beneficial to use a panel of monoclonal antibodies of different clones covering different epitopes for more sensitive ...

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Assessment of vascular endothelial growth factor in formalin fixed, paraffin embedded colon cancer specimens by means of a well-based reverse phase protein array

Assessment of vascular endothelial growth factor in formalin fixed, paraffin embedded colon cancer specimens by means of a well-based reverse phase protein array

... well-based reverse phase protein array (RPPA) and immunohistochemistry in 69 colon cancer cases, and compared with various clinicopathologic ...factors. Protein lysates derived from ...

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HER-2 assessment in formalin-fixed paraffin-embedded breast cancer tissue by well-based reverse phase protein array

HER-2 assessment in formalin-fixed paraffin-embedded breast cancer tissue by well-based reverse phase protein array

... Reverse phase protein array (RPPA) is a sensitive and high throughput technology that allows the quantification of given makers in small amount of protein from biological specimens ...

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The impact of ultraviolet- and infrared-based laser microdissection technology on phosphoprotein detection in the laser microdissection-reverse phase protein array workflow

The impact of ultraviolet- and infrared-based laser microdissection technology on phosphoprotein detection in the laser microdissection-reverse phase protein array workflow

... to reverse-phase protein array (RPPA) has generated highly accurate and reliable phosphoprotein data from FFPE and frozen tissues [6, 8, 27, ...

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Semi-quantitative measurement of specific proteins in human cumulus cells using reverse phase protein array

Semi-quantitative measurement of specific proteins in human cumulus cells using reverse phase protein array

... the protein of interest was normalised by the signal of VCL, which was used as a protein of ref- erence, or by the signal of ERK2 (for the validation of anti-VCL) to calculate the relative expression ...the ...

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Reverse phase protein arrays in signaling pathways: a data integration perspective

Reverse phase protein arrays in signaling pathways: a data integration perspective

... The reverse phase protein array (RPPA) data platform provides expression data for a prespecified set of proteins, across a set of tissue or cell line ...

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A Technical Assessment of the Utility of Reverse Phase Protein Arrays for the Study of the Functional Proteome in Non-microdissected Human Breast Cancers

A Technical Assessment of the Utility of Reverse Phase Protein Arrays for the Study of the Functional Proteome in Non-microdissected Human Breast Cancers

... Fig. 1 a Akt and b Aktp473 antibody validation for reverse phase protein array (RPPA). MDAMB468 (red), ZR75-1 (black) and T47D (blue) cells were left untreated followed by no stimulation ...

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Integrative proteomic and gene expression analysis identify potential biomarkers for adjuvant trastuzumab resistance: analysis from the Fin-her phase III randomized trial

Integrative proteomic and gene expression analysis identify potential biomarkers for adjuvant trastuzumab resistance: analysis from the Fin-her phase III randomized trial

... and reverse-phase protein arrays) ...and protein expression data from Reverse-Phase Protein Array Analysis (RPPA) in HER2+ breast cancer from the TCGA repository, ...

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Development of reverse phase protein microarrays for the validation of clusterin, a mid-abundant blood biomarker

Development of reverse phase protein microarrays for the validation of clusterin, a mid-abundant blood biomarker

... quality array data in the validation analysis we filtered for spot quality (see ...the protein microarray were significantly higher in serum samples when compared to CTAD (p < ...inter- array ...

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An efficient procedure for protein extraction from formalin-fixed, paraffin-embedded tissues for reverse phase protein arrays

An efficient procedure for protein extraction from formalin-fixed, paraffin-embedded tissues for reverse phase protein arrays

... partial protein degradation on Coomassie staining and western blots, we proceeded with RPPA, in order to evaluate protein expression in extracts processed with method ...

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Development of multiplexed protein profiling and detection using near infrared detection of reverse-phase protein microarrays

Development of multiplexed protein profiling and detection using near infrared detection of reverse-phase protein microarrays

... total protein levels in each ...total protein levels of important signal pathway mediators such as ERK kinase from clinical material on a single array and within the same ...

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Quantitative analysis of proteins of metabolism by reverse phase protein microarrays identifies potential biomarkers of rare neuromuscular diseases

Quantitative analysis of proteins of metabolism by reverse phase protein microarrays identifies potential biomarkers of rare neuromuscular diseases

... Each array was incu- bated with each antibody ...expressed protein/ ng of total protein in the tissue extract using the expres- sion obtained in the linear plot of the HCT116 cell line as ...

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Improvement of Phase Noise Performance in Tracking Array of UAV Signal Based on Mixed Phased/Retrodirective Array

Improvement of Phase Noise Performance in Tracking Array of UAV Signal Based on Mixed Phased/Retrodirective Array

... all array elements (local oscillators, quadrature modulators/demodulators) and element dislocation contribute, directly or indirectly, to increasing the phase errors ...total phase errors as one ...

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Phase Transformation of α'' Martensite Structure by Aging in Ti 8 mass%Mo Alloy

Phase Transformation of α'' Martensite Structure by Aging in Ti 8 mass%Mo Alloy

... phases. Therefore, isothermal aging treatments for the quenched specimens were performed at 723 K or 923 K that corresponded to the state of before or after the phase decomposition respectively, for 9.0 ks. In ...

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The Axial Ratio of Planar Arrays with Random Element Errors

The Axial Ratio of Planar Arrays with Random Element Errors

... an array are considered statistically independent from element to ...and phase errors are not. These errors produce very broad array patterns that are superimposed on the no-error patterns resulting ...

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A Gain-Phase Error Calibration Method for the Vibration of Wing Conformal Array

A Gain-Phase Error Calibration Method for the Vibration of Wing Conformal Array

... the array deformed shape curve is fitted through the gain error of the array, thus the position of each element can be ...the array, and effectively compensate the position error caused by the ...

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Array antennas design in dependence of element-phasing

Array antennas design in dependence of element-phasing

... the array phase is useful in many areas, even if it is an artificial array like a synthetic aperture radar ...the phase with charging Doppler ...full phase characteristics including ...

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Reverse phase chromatographic behaviour of major components in Capsicum Annuumextract

Reverse phase chromatographic behaviour of major components in Capsicum Annuumextract

... Capsaicin subject hydrogenation turns dihidrocapsaicin [2]. Capsaicin is a capsaicinoid alkaloid with notable thermal stability retaining its activity even boiling [3]. It is only slightly soluble in water [4], but very ...

6

Parametric Acoustic Receiving Array in Two phase Media

Parametric Acoustic Receiving Array in Two phase Media

... receiving array can achieve higher space gain with relatively small (virtual) array aperture compare with ordinary line ...receiving array is proportional to the third power of the acoustic velocity ...

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Method Development and Validation for the Estimation of Decitabine in Pharmaceutical Dosage form by RP-HPLC Method

Method Development and Validation for the Estimation of Decitabine in Pharmaceutical Dosage form by RP-HPLC Method

... Elevated temperatures decrease viscosity and increase solubility and diffusivity. Retention, peak shape, column efficiency, and total analysis time are affected by temperature because both the thermodynamics and kinetics ...

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