Reverse transcriptase-polymerase chain reaction (RT-PCR)
A comparative analysis of routine techniques: Reverse transcriptase polymerase chain reaction (RT-PCR) and five cell lines for detection of enteroviruses in stool specimens
... detection. RT-PCR (Reverse Transcriptase Polymerase Chain Reaction) is a technique used in place of the cell culture ...with RT-PCR for detection of ...
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Heminested reverse transcriptase polymerase chain reaction (hnRT PCR) as a tool for rabies virus detection in stored and decomposed samples
... disease. Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) has been used in rabies diagnosis with good results, even in decomposed ...
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RT-PCR Fact Sheet Real Time Reverse Transcription Polymerase Chain Reaction (Real Time RT-PCR) Test.
... transcription PCR, or ...is reverse transcribed into complementary DNA, using reverse ...of RT-PCR. Real time RT-PCR is a method for detecting the presence of specific ...
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Improved methods using the reverse transcriptase polymerase chain reaction to detect tumour cells
... efficiency limits the amount of RNA and consequently blood volume that may be analysed in any single PCR. Amplification of poly-A + RNA isolated from whole blood total RNA was more sensitive than analysis of total ...
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Detection of Norwalk virus in stool specimens by reverse transcriptase polymerase chain reaction and nonradioactive oligoprobes
... For agarose gels, 21 coded stool specimens were purified by Sephadex G-200 spin-column chromatography, amplified by RT-PCR by using Norwalk virus NV-5'Pol and NV-3'Pol primers (left lane[r] ...
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An analysis of the subtypes of dengue fever infections in Barbados 2003–2007 by reverse transcriptase polymerase chain reaction
... the RT-PCR will enhance the detection particularly where the IgM and IgG antibodies are undetectable by ELISA especially for those samples that under 5 days after symptom ...
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Canine Distemper Virus detection based in Hemaglutinine Gene as target in Reverse Transcriptase-Polymerase Chain Reaction
... The RT-PCR technique for the detection of the hemagglutinin gene was selected because this protein expressed by the virus is responsible for generating the response immune in the organism, besides being ...
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Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase polymerase chain reaction
... As previously stated, the RNA isolated from these infected mosquitoes was originally found negative for dengue type 2 virus by the RT-PCR assay at all time points. However, when the RNA [r] ...
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Detection of Rhinovirus, Respiratory Syncytial Virus, and Coronavirus Infections in Acute Otitis Media by Reverse Transcriptase Polymerase Chain Reaction
... by RT-PCR in 48% of MEF samples and in 75% of children with ...by RT-PCR in 24% of MEF samples and evidence of HRV infection in 35% of children with ...
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Deteksi Dan Identifikasi Chrysanthemum Stunt Viroid Pada Tanaman Krisan Menggunakan Teknik Reverse Transcriptase Polymerase Chain Reaction (Detection and Identification of Chrysanthemum Stunt Viroid on Chrysanthemum Using Reverse Transcriptase Polymerase
... ABSTRAK. Chrysanthemum stunt viroid (CSVd) merupakan salah satu viroid yang menginfeksi tanaman krisan di Indonesia. Tujuan penelitian ialah untuk mengembangkan metode deteksi CSVd secara molekuler dan mengkarakterisasi ...
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Reverse Transcriptase Pcr Protocol Pdf
... is reverse transcriptase pcr protocol ...initial pcr? This is often accomplished by comparison to a standard curve generated using known genome equivalents or nucleic acid harvested from a ...
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A duplex real time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses
... real-time RT-PCR assay by adjusting the concentrations of primers and ...time RT-PCR assay we developed was 10-fold more sensitive for both WEEV and EEEV ...
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Her2 assessment using quantitative reverse transcriptase polymerase chain reaction reliably identifies Her2 overexpression without amplification in breast cancer cases
... Technical limitations accounting for part of the dis- crepancies of molecular tests can be circumvented by a proper methodological standardization. The puta- tive dilution or amplification effects are theoretically more ...
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Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real time reverse transcriptase polymerase chain reaction to predict recurrence of gastric adenocarcinoma
... traditional PCR technol- ogy has made it difficult to differentiate baseline gene expression levels in normal tissues from increased gene expression levels in cancer, thereby increasing the con- cern for ...
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A novel duplex real time reverse transcriptase polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control
... real-time RT-PCR for the 109 serum samples, which were identical to those of the CAP/CTM assay, should be ...real-time RT-PCR assay was ...real-time RT-PCR assay was considerably ...
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Utility of a multiplex reverse transcriptase-polymerase chain reaction assay (HemaVision) in the evaluation of genetic abnormalities in Korean children with acute leukemia: a single institution study
... include polymerase chain reac- tion (PCR)-based molecular methods, and cytogenetic studies including fluorescence in situ hybridization (FISH) tests and con- ventional G-band ...multiplex ...
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Application of in situ reverse trancriptase-polymerase chain reaction (RT-PCR) to tissue microarrays
... or PCR amplification of reverse-transcribed ...for PCR, the microarray presents significant economies of scale and can equal or better the specificity of other techniques, in- cluding the detection ...
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The reverse-transcription polymerase chain reaction (RT-PCR) may be used to detect viral RNA.
... substrate/chromogen reaction is stopped by addition of a suitable stopping solution and the colour reactions are quantified by measuring the optical density of each ...
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SCIENTIFIC CONCEPTS OF POLYMERASE CHAIN REACTION (PCR)
... of PCR technology has substantially increased our powers of biological discrimination particularly in rape ...of PCR in forensic analyses has largely proved to be exaggerated, with even degraded samples ...
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