reverse transcription and polymerase chain reaction
Predominant expression of beta 1 adrenergic receptor in the thick ascending limb of rat kidney Absolute mRNA quantitation by reverse transcription and polymerase chain reaction
... Beta 1- and beta 2-adrenergic receptor (beta-ARs) expression in the thick ascending limb of rat kidney was studied at the level of mRNA and receptor coupling to adenylyl cyclase. Absolute quantitation of beta 1- and beta ...
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Simultaneous detection and differentiation of three genotypes of Brassica yellows virus by multiplex reverse transcription polymerase chain reaction
... multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed for simultaneous detection and differentiation of the three genotypes of ...
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Diagnosis of Foot-and-Mouth Disease Virus by Real Time Reverse Transcription Polymerase Chain Reaction Assay in Iran
... various reverse transcription polymerase chain reaction (RT- PCR) methods are used to detect and identity FMDV isolations ...each reaction series can not be used (13). ...
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Use of the Reverse Transcription-Polymerase Chain Reaction for differential detection of two lineages of the canine distemper virus in Chile
... called Reverse Transcription Polymerase Chain Reaction (RT-PCR) has been used to characterize viral strains based on the basis of genetic differences on the hemagglutinin (H) gene of ...
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Reverse transcription and polymerase chain reaction amplification of rRNA for detection of Helicobacter species
... When crude nucleic acid extracts prepared from two of the above culture-positive gastric biopsy specimen homogenates were used as templates in RT-PCR, the expected amplified fragment fro[r] ...
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Different localization of two types of endothelin receptor mRNA in microdissected rat nephron segments using reverse transcription and polymerase chain reaction assay
... reaction product were detected in the initial and terminal inner medullary collecting duct and the glomerulus, while small signals were found in the cortical collecting duct and outer medullary collecting duct, ...
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Novel variants of citrus exocortis viroid from southern of Iran
... volume polymerase chain reaction. Reverse transcription and polymerase chain ...of reverse primer (5′- CCGGGGATCCCTGAAGGA-3′) ...
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Isolation and expression characterization of CBF2 in vitis amurensis with stress
... Quantitative reverse-transcription polymerase chain reaction analysis indicated that the expression of VaCBF2 gene in tissues roots, stems, leaves, and petioles was induced by low temper[r] ...
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... quantitative reverse transcription polymerase chain reaction in the cells transfected with miR-466 mim- ics compared to Neg-miR-transfected cells (p < ...
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Uteroglobin gene expression in the rabbit uterus throughout gestation and in the fetal lung Relationship between uteroglobin and eicosanoid levels in the developing fetal lung
... Using reverse transcription polymerase chain reaction, in situ hybridization, immunofluorescence, and radioimmunoassay, we studied UG gene expression in the rabbit uterus throughout ...
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Original Article Chronic stress reduces spermatogenic cell proliferation in rat testis
... quantitative reverse transcription polymerase chain reaction (RT-qPCR) and immunohisto- chemistry, we investigated the changes in tes- ticular spermatogenic cell proliferation and ...
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Serum osteopontin: a biomarker of disease activity and predictor of relapsing course in patients with giant cell arteritis Potential clinical usefulness in tocilizumab treated patients
... Background Osteopontin (OPN) is a glycoprotein involved in Th1 and Th17 differentiation, tissue inflammation and remodelling. We explored the role of serum OPN (sOPN) as a biomarker in patients with giant cell arteritis ...
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Upregulation of inflammatory genes in the nasal mucosa of patients undergoing endonasal dacryocystorhinostomy
... Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed for the inflammatory genes interleukin (IL)-6, IL-1β, and CCL2, and because of a clear trend of ...
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Real Time PCR and Real Time RT PCR applications in food labelling and gene expression studies
... Abstract: Polymerase chain reaction (PCR) as a scientific invention, has revolutionized molecular biology and led to real-time PCR and later, real-time reverse transcription PCR ...
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A mixture of the aqueous extract of Garcinia cambogia, soy peptide and l-carnitine reduces the accumulation of visceral fat mass in rats rendered obese by a high fat diet
... real-time reverse transcription-polymerase chain reaction analyses indicated that the expression levels of leptin, tumor necrosis factor-alpha (TNF-a), and sterol regulatory element ...
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A New Sensitive Method for Detection of Viroids
... and reverse transcription coupled with polymerase chain reaction (RT-PCR) have become popular for the practical diagnosis of viroids because of their relative simplicity and high ...
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Efficacy of D5F3 IHC for detecting ALK gene rearrangement in NSCLC patients: a systematic review and meta-analysis
... Immunohistochemistry, Fluorescence in Situ Hybridization, and Reverse Transcription-Polymerase Chain Reaction for the Detection of Anaplastic Lymphoma Kinase Gene Rearrangements in Patie[r] ...
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ALK (D5F3) CDx: an immunohistochemistry assay to identify ALK-positive NSCLC patients
... Immunohistochemistry, fluo- rescence in situ hybridization, and reverse transcription-polymerase chain reaction for the detection of anaplastic lymphoma kinase gene rearrangements in p[r] ...
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Citrus Bent Leaf Viroid from Fars Province of Iran
... Reverse transcription polymerase chain ...the reverse transcription polymerase chain reaction with a specific primer of citrus bent leaf viroid led to ...
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Sulfur mustard induces expression of metallothionein-1A in human airway epithelial cells
... all the RNA contained in the airway biopsy specimens was harvested in Tripure isolation reagent in accordance with the manufacturer’s protocol and kept at − 80 ° C during the procedure. The RNA extracted was evaluated by ...
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