Reverse transcription PCR
Quantitative Reverse Transcription Pcr
... AMV Reverse Transcriptase, ...quantitative pcr primer annealing, about the template for in the rna resources for a ...and pcr reaction samples isolated from different types of the temperature in the ...
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Reverse transcription PCR detection of hepatitis G virus
... the reverse transcription-PCR (RT- PCR) assay with the Enzymun-Test DNA detection system (Boehringer Mannheim GmbH, Mannheim, Germany) and made this technique applicable for mass screening in ...
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Standardized positive controls for detection of norovirus by reverse transcription PCR
... Abstract Background: Norovirus is one of the most common causes of nonbacterial gastroenteritis in humans. Rapid spread by contaminated food and person-to-person transmission through the fecal-oral route are ...
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Quantitative Competitive Reverse Transcription PCR for Quantification of Dengue Virus RNA
... Received 11 February 2000/Returned for modification 19 May 2000/Accepted 5 July 2000 A quantitative competitive reverse transcription-PCR assay was developed to quantify dengue virus RNA in this ...
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Multiplex Reverse Transcription PCR for Simultaneous Surveillance of Influenza A and B Viruses
... multiplex reverse transcription-PCR (RT- PCR) method that amplifies the most critical genomic segments (hemagglutinin [HA], neuraminidase [NA], and matrix [M]) of seasonal influenza A and B ...
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Duplex Reverse Transcription PCR Followed by Nested PCR Assays for Detection and Identification of Brazilian Alphaviruses and Flaviviruses
... duplex reverse transcription-PCR (D-RT-PCR), followed by multiplex nested PCR (M-N-PCR) or nested PCR (N- PCR) assays for species-specific ...
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Development of Reverse Transcription PCR Assays Specific for Detection of Equine Encephalitis Viruses
... sensitive reverse transcription-PCR (RT-PCR) assays were developed for the detection of eastern, western, and Venezuelan equine encephalitis viruses (EEE, WEE, and VEE, ...nested PCR, ...
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Determination of Human Rotavirus VP6 Genogroups I and II by Reverse Transcription PCR
... Based on nucleotide sequence and phylogenetic analysis of the partial VP6 genes, group A rotaviruses can be mainly differentiated into two genogroups. In this study, a method employing reverse ...
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Sensitive and Broadly Reactive Reverse Transcription PCR Assays To Detect Novel Paramyxoviruses
... of reverse transcription-PCR assays for the detection and identification of known and novel paramyxoviruses in clinical ...nested PCR assay design were used to enhance the breadth of ...
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Real Time Reverse Transcription PCR Assay for Comprehensive Detection of Human Rhinoviruses
... real-time reverse transcription-PCR (RT-PCR) assay targeting the viral 5 ⴕ noncoding region defined by sequences obtained from all 100 currently recognized HRV prototype strains and 85 ...
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Critical Analysis of Rhinovirus RNA Load Quantification by Real Time Reverse Transcription PCR
... real-time reverse-transcription PCR (RT-PCR) assays are fre- quently converted to viral RNA loads, this presents several limitations regarding accurate virus RNA quantification, particularly ...
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Detection of Tilapia Lake Virus in Clinical Samples by Culturing and Nested Reverse Transcription PCR
... ABSTRACT Tilapia are an important group of farmed fish that serve as a significant protein source worldwide. In recent years, substantial mortality of wild tilapia has been observed in the Sea of Galilee and in commercial ...
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Identification of 20 Common Human Enterovirus Serotypes by Use of a Reverse Transcription PCR Based Reverse Line Blot Hybridization Assay
... on reverse transcription-PCR (RT-PCR) amplification and nucleotide sequencing of the entire or 3 ⴕ half of the VP1 ...RT-PCR-based reverse line blot (RLB) hybridization assay was ...
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Applications of Competitor RNA in Diagnostic Reverse Transcription PCR
... Detection of RNA viruses by reverse transcription (RT)-PCR has proven to be a useful approach for the diagnosis of infections caused by many viral pathogens. However, adequate controls are required ...
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Reverse Transcription PCR Detection ofMycobacterium leprae in Clinical Specimens
... Other investigators (23) have indicated that PCR inhibitors were present in some of their skin samples, particularly those with heavy infiltration. Inhibitors are less likely to influence our assay because our ...
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Detection of Human Picornaviruses by Multiplex Reverse Transcription PCR and Liquid Hybridization
... multiplex reverse transcription (RT)-PCR and liquid hybridization assay for the detection of human enteroviruses, rhinoviruses, parechoviruses, and Aichi virus was ...
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Enhanced Reverse Transcription PCR Assay for Detection of Norovirus Genogroup I
... nested PCR. We describe a real-time RT-PCR assay for NV GGI which is based on LNA probe ...of PCR when shorter detection probes are used, due to lower interference during the amplification pro- cess ...
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Development of one step quantitative reverse transcription PCR for the rapid detection of flaviviruses
... risk of contamination caused by open handling of PCR products, increasing the potential for false positives. In contrast, the Pan-Flavi assay presented here requires only some 50 minutes for specific and sensitive ...
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Automated quantitative determination of hepatitis C virus viremia by reverse transcription PCR
... positive serum sample with an unknown viral copy number, reverse transcribed into cDNA, and amplified five times along with an increasing number of mimic RNAs ranging from 50 to 5 x 105 [r] ...
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Use of Bacteriophage MS2 as an Internal Control in Viral Reverse Transcription PCR Assays
... on reverse transcription (RT)-PCR are widely used for the detection of viral genomes in different human ...extraction, reverse transcription, and amplification steps. The MS2 ...
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