Reversed-phase chromatography with protamine sulphate
Reversed Phase Chromatography
... Although it is not possible to predict theoretically which ligand will be best for a particular application, a good rule of thumb is: the more hydrophobic the molecule to be purified, the less hydrophobic the ligand ...
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Comparison of the determination of folate vitamers with hydrophilic interaction chromatography and reversed phase chromatography
... conventional reversed phase ...interaction chromatography, short HILIC, could be the preferred choice of ...to reversed phase liquid chromatography with the benefit of being able ...
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Reversed-phase thin-layer chromatography behavior of aldopentose derivatives
... by reversed-phase thin-layer chromatography on paraf- fin oil-impregnated silica gel plates, with acetone–wa- ter, dioxane–water and methanol–water as mobile ...
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Determination of Carbonyl Compounds by Reversed-Phase High-Performance Liquid Chromatography
... The derivatized samples are extracted with methylene chloride and the extracted compounds exchanged into acetonitrile, followed by HPLC analysis.... 4 Determination of Carbonyl Compounds[r] ...
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GE Healthcare. Hydrophobic Interaction and Reversed Phase Chromatography. Principles and Methods
... liquid chromatography (MDLC) The Cipp strategy is well suited for purification of a target protein or ...However, chromatography techniques are also successfully combined to achieve identification and ...
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Development and validation of reversed-phase high performance liquid chromatographic method for hydroxychloroquine sulphate
... simple reversed‑phase high performance liquid chromatographic method was developed and validated for the determination of hydroxychloroquine sulphate in blood ...Chloroquine sulphate was used ...
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STUDY OF FACTORS AFFECTING ON SOLVENT EFFECT AND PEAK SHAPE IN REVERSED PHASE CHROMATOGRAPHY
... Detection wavelength: 254mn, flow rate: 1ml / min, column temperature: 30 ℃, injection volume: 10μl. Discussion and Analysis Significant differences in both the reduced and non-reduced axial diffusion behaviour of ...
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Simultaneous determination of naproxen and diphenhydramine by reversed phase liquid chromatography and derivative spectrophotometry
... accurate reversed phase liquid chromatographic and first derivative spectrophotometric methods were developed for determination of the non-steroidal anti-inflammatory drugnaproxen and the antihistaminic ...
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Determination of Letrozole in Tablet Formulations by Reversed Phase High Performance Liquid Chromatography
... one week, increased to room temperature and then injected into the HPLC system. Assay sample preparation A uniform mixture of tablet powder was obtained by powdering and mixing twenty tablets. From this powder mixture, ...
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Reversed phase C-18 chromatography: From analytical scale to preparative scale
... flash chromatography instrument — the CombiFlash Companion — and RediSep reversed phase C-18 flash columns offer chemists an easier and efficient alternative to pre- parative HPLC ...RediSep ...
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EVALUATION OF THE USE OF ANIONIC/ NONIONIC MIXED MICELLES IN REVERSED PHASE LIQUID CHROMATOGRAPHY OF CHLOROPHENOLS
... Institute of Chemistry, Mazandaran University, P.O. Box 453, Babolsar, Islamic Republic of Iran Abstract The use of aqueous mixed micellar system consisting of sodium dodecyl sulfate (SDS) and polyoxyethylene (23) ...
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Quantitative structure retention relationships for rapid method development in reversed phase liquid chromatography
... The primary objective in this study of QSRR modelling of retention time was its subsequent use for predicting retention times of compounds based on their chemical structure. Since Dataset 1 involves 88 compounds and 10 ...
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Study of forced degradation behavior of fluorometholone by reversed-phase high-performance liquid chromatography
... Department of Pharmaceutical Analysis and Quality Assurance, GITAM Institute of Pharmacy, GITAM University, Visakhapatnam, Andhra Pradesh, India Abstract Introduction: The present proposed work describes the development ...
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Simultaneous determination of Atenolol, Amiloride hydrochloride and Hydrochlorothiazide using reversed phase liquid chromatography
... precise reversed phase liquid chromatographic method has been developed and validated for the simultaneous determination of atenolol, amiloride hydrochloride and ...mobile phase consisting of 25 mM ...
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Reversed phase liquid chromatography with mass detection and characterization of saxagliptin degradation related impurities
... 511 combinations involving methanol, acetonitrile, buffer solution (Ammonium acetate) were tried, in isocratic and gradient modes. Finally, good separation and peak shape where observed with the use of water and ...
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Separation Of Phenylarsonic Compounds By Ion Pairing-Reversed Phase-High Performance Liquid Chromatography
... p-ASA, PA and 3-NHPAA are all dissociated at the pH range studied. These compounds contain acidic functional groups and their dissociations depend on the pK a and mobile phase pH. The chromatograms presented in ...
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REVERSED PHASE LIQUID CHROMATOGRAPHY WITH MASS DETECTION AND NMR CHARACTERIZATION OF SITAGLIPTIN DEGRADATION RELATED IMPURITIES
... liquid chromatography (HPLC) reveals a few selected methods for the determination of Sitagliptin either single or in combination with some other drugs like, metformin, simvastatin, ...
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Performance Study Of Carbon-Clad Zirconia Stationary Phase In High Temperature Reversed-Phase High Performance Liquid Chromatography
... stationary phase using mobile phases with low organic modifier composition at elevated temperatures of 100°C to 160°C has been ...stationary phase is not only hydrophobic but also retains solutes through ...
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Practical observations on the performance of bare silica in hydrophilic interaction compared with C18 reversed phase liquid chromatography
... mobile phase viscosity might contribute pos- itively to fast analysis, since higher linear flow velocities can be adopted within the pressure limit of the ...
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Reversed-phase high performance liquid chromatography study of lipophilicity of imidazo[2,1-f]theophylline derivatives
... using reversed-phase high-performance liquid chromatog- raphy (RP-HPLC) ...mobile phase and the chromatographic properties of the investigated compounds; as well as the influence of substituent on ...
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