ribosomal DNA (rDNA)
Species Identification and Strain Differentiation of Dermatophyte Fungi by Analysis of Ribosomal DNA Intergenic Spacer Regions
... the ribosomal-DNA (rDNA) repeat were used for molecular strain differentiation of the dermatophyte fungus Trichophyton ...(18S) rDNA and adjacent internal transcribed spacer (ITS) regions. The ...
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Assessment of genetic diversity among different sugarcane genotypes using internal transcribed spacer (ITS) region of the ribosomal DNA (rDNA)
... nuclear ribosomal DNA (rDNA) has been used to evaluate genetic assortment and phylogenetic relationship in nine sugarcane genotypes including Saccharum species and another related genus as Erianthus ...
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Usefulness of the MicroSeq 500 16S Ribosomal DNA Based Bacterial Identification System for Identification of Clinically Significant Bacterial Isolates with Ambiguous Biochemical Profiles
... The MicroSeq 500 16S ribosomal DNA (rDNA)-based bac- terial identification system (Perkin-Elmer Applied Biosystems Division, Foster City, Calif.) has been designed for rapid and accurate ...
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Bacterial Diversity in Cases of Lung Infection in Cystic Fibrosis Patients: 16S Ribosomal DNA (rDNA) Length Heterogeneity PCR and 16S rDNA Terminal Restriction Fragment Length Polymorphism Profiling
... By assessing nucleic acids extracted from clinical samples directly, molecular biology-based assays obviate the require- ment for cultivation. As such, these approaches are becoming increasingly important and are finding ...
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Molecular Typing of Papillomatous Digital Dermatitis Associated Treponema Isolates Based on Analysis of 16S 23S Ribosomal DNA Intergenic Spacer Regions
... 16S ribosomal DNA (rDNA) sequence analysis of clonal isolates from California cattle showed that they comprise three phylotypes which cluster closely with human-associated Treponema ...16S-23S ...
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Characterization of Mycobacterium bohemicum Isolated from Human, Veterinary, and Environmental Sources
... Chemotaxonomic and genetic properties were determined for 14 mycobacterial isolates identified as mem- bers of a newly described species Mycobacterium bohemicum. The isolates recovered from clinical, veterinary, and ...
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Molecular confirmation of the occurrence in Germany of Anopheles daciae (Diptera, Culicidae)
... nuclear ribosomal DNA (rDNA) second in- ternal transcribed spacer (ITS2), Harbach [3] confirmed the monophyly of the Maculipennis group species in 2004 and divided them into three hierarchical ...
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Genetic and molecular organization of ribosomal DNA (rDNA) variants in wild and cultivated barley.
... * Number of individuals assayed in each family. and in H.V. A hybrid with a true-breeding plant with three slvs: Some of the plants with three slvs that have been found in H[r] ...
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Phenotypic and Genotypic Consequences of CRISPR/Cas9 Editing of the Replication Origins in the rDNA of Saccharomyces cerevisiae
... eukaryotic ribosomal DNA (rDNA) is a challenge for genome assembly, thus the consequences of sequence variation in rDNA remain ...that rDNA variation may play in diverse cellular ...
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Candida inconspicua and Candida norvegensis: New Insights into Identification in Relation to Sexual Reproduction and Genome Organization
... of ribosomal DNA sequences, notably, the D1/D2 do- main of the large subunit and the ITS loci, is considered a method of choice for the identification of ascomycetous yeast species (17, ...The rDNA ...
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Molecular Variability of Pseudallescheria boydii, a Neurotropic Opportunist
... 18S ribosomal DNA (rDNA) sequencing ...using rDNA ITS2 sequencing, were able to select primers for species ...(ITS) rDNA of ...nuclear DNA (nDNA) homology studies had indicated ...
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Phylogenetic analysis of oyster mushrooms (Pleurotus spp.) based on restriction fragment length polymorphisms of the 5′ portion of 26S rDNA
... 26S ribosomal DNA (26S rDNA) from 34 Pleurotus strains, including 12 intersterility groups (biological species) col- lected mainly from Asia, were examined by polymerase chain reaction-RFLP ...
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Misidentifying Helicobacters: theHelicobacter cinaedi Example
... 16S ribosomal DNA (rDNA) sequence similarity to known species (for example, reference 13), or as well-established species, again primarily based on the percent- age of similarity of 16S rDNA ...
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Biodiversity, community structural shifts, and biogeography of prokaryotes within antarctic continental shelf sediment
... 16S ribosomal DNA (rDNA) clone library analysis was conducted to assess prokaryotic diversity and community structural changes within a surficial sediment core obtained from an Antarctic continental ...
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Evolution of ribosomal DNA (rDNA) genetic structure in colonial Californian populations of Avena barbata.
... We conclude that the genetic structuring observed in the colonial populations of the present study resulted from interplay among several evolutionary forces among which[r] ...
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Differentiation of Burkholderia Species by PCR Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene and Application to Cystic Fibrosis Isolates
... Burkholderia cepacia, which is an important pathogen in cystic fibrosis (CF) owing to the potential severity of the infections and the high transmissibility of some clones, has been recently shown to be a complex of five ...
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Multiplex PCR Using Internal Transcribed Spacer 1 and 2 Regions for Rapid Detection and Identification of Yeast Strains
... Multiplex PCR amplification followed by either agarose gel electrophoresis (PCR-AGE) or microchip electrophoresis (PCR-ME) was used to test a total of 120 fungal strains. The internal transcribed spacer 1 (ITS1) and ITS2 ...
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Discrimination of Aspergillosis, Mucormycosis, Fusariosis, and Scedosporiosis in Formalin Fixed Paraffin Embedded Tissue Specimens by Use of Multiple Real Time Quantitative PCR Assays
... of ribosomal DNA, using fluorescently labeled primers, was performed to identify clinically important genera and species of Aspergillus, Fusarium, Scedosporium, and the ...automated DNA extraction ...
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Homogenisation in the ribosomal RNA genes of an Epichloë endophyte hybrid : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Molecular Genetics at Massey University, Palmerston North, New Zealand
... of rDNA unit number using a variety of parameters and found a number of ways the parameters could be manipulated to fit the experimental ...which rDNA transcription efficiency decreases (Coen and Dover, 1 ...
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Phylogenetic Analysis of Lacazia loboiPlaces This Previously Uncharacterized Pathogen within the Dimorphic Onygenales
... Although L. loboi and P. brasiliensis are close relatives, there are significant differences between the two fungi, not only at the DNA level but also in the diseases that they cause. While P. brasiliensis clearly ...
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