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RNA on the X

Disruption of a conserved region of Xist exon 1 impairs Xist RNA localisation and X linked gene silencing during random and imprinted X chromosome inactivation

Disruption of a conserved region of Xist exon 1 impairs Xist RNA localisation and X linked gene silencing during random and imprinted X chromosome inactivation

... Xist RNA there are disadvantages to this ...the X chromosome, the basis of the silencing assay used by Wutz et ...Xist RNA mutations being scored as ...non-random X inactivation of the ...

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Template Requirements for De Novo RNA Synthesis by Hepatitis C Virus Nonstructural Protein 5B Polymerase on the Viral X RNA

Template Requirements for De Novo RNA Synthesis by Hepatitis C Virus Nonstructural Protein 5B Polymerase on the Viral X RNA

... the RNA template for the RdRp reaction, we found that X RNA does not satisfy all of these require- ments in its secondary structure or nucleotide ...to X RNA for the RdRp ...native ...

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The 5' nontranslated region of potato virus X RNA affects both genomic and subgenomic RNA synthesis.

The 5' nontranslated region of potato virus X RNA affects both genomic and subgenomic RNA synthesis.

... virus X (PVX) ...for RNA production by S1 nuclease protec- tion ...of RNA levels in protoplasts inoculated with PVX clones containing deletions or an insertion in their 5 * ...

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Determining the role of the Xist non-coding RNA in epigenetic programming of X-chromosome inactivation

Determining the role of the Xist non-coding RNA in epigenetic programming of X-chromosome inactivation

... multiple X-linked genes. All RNA FISH probes used to detect X-linked genes were tested in trophoblast (TS) cell lines to determine detection of X-linked sequences with expected expression ...

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Hepatitis B virus x protein induces epithelial mesenchymal transition of hepatocellular carcinoma cells by regulating long non coding RNA

Hepatitis B virus x protein induces epithelial mesenchymal transition of hepatocellular carcinoma cells by regulating long non coding RNA

... Hepatitis B virus x protein induces epithelial mesenchymal transition of hepatocellular carcinoma cells by regulating long non coding RNA RESEARCH Open Access Hepatitis B virus x protein induces epith[.] ...

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Dynamics of gene silencing during X inactivation using allele specific RNA seq

Dynamics of gene silencing during X inactivation using allele specific RNA seq

... of X ‐ linked genes in ES_Tsix ‐ stop ESCs maintained in serum ‐ free media in the presence of 2 kinase inhibitors ( “ 2i ” ) compared with ES_Tsix ‐ stop ESCs maintained under serum ...by RNA expression of ...

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The Enigma of Double-stranded RNA (dsRNA) Associated with Mushroom Virus X (MVX)

The Enigma of Double-stranded RNA (dsRNA) Associated with Mushroom Virus X (MVX)

... translated RNA dependent RNA polymerase (RdRP) regions had high similarity with other members of the ...dependent RNA Polymerase) sequences of MVX-mushroom dsRNAs (Rao et ...

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RNA synthesis in cells infected with herpes simplex virus. X. Properties of viral symmetric transcripts and of double-stranded RNA prepared from them.

RNA synthesis in cells infected with herpes simplex virus. X. Properties of viral symmetric transcripts and of double-stranded RNA prepared from them.

... Suggestive evidence that complementary, self-annealing viral RNA sequences exist in HSV-1-infected cells emerged from the observations that nuclear RNA from 8-h infected cells preanneale[r] ...

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GENETIC MODULATION OF RNA METABOLISM IN DROSOPHILA. III. REQUIREMENT FOR AN rDNA-DEFICIENT X CHROMOSOME IN YbbSuVar–5-MEDIATED INCREASES IN RNA SYNTHESIS

GENETIC MODULATION OF RNA METABOLISM IN DROSOPHILA. III. REQUIREMENT FOR AN rDNA-DEFICIENT X CHROMOSOME IN YbbSuVar–5-MEDIATED INCREASES IN RNA SYNTHESIS

... We began the experiments to determine if Ybbsuvar-5 has an effect on RNA metab- olism in a genotype that has one wild-type nucleolus organizer by measuring the rDNA content of app[r] ...

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RNA synthesis of vesicular stomatitis virus. X. Transcription and replication by defective interfering particles.

RNA synthesis of vesicular stomatitis virus. X. Transcription and replication by defective interfering particles.

... Although the bulk of intracellular RNA synthesis specific to DI particles is known to be under replicative and not transcriptive control 23, it is not clear whether this small RNA is the[r] ...

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Low RNA Binding Strength of Human X Chromosome May Explain X Chromosome Inactivation

Low RNA Binding Strength of Human X Chromosome May Explain X Chromosome Inactivation

... the X chromosome in eutherians and the copy number of L1 in X chromosome was higher than that in the autosomes ...one X chromosome, approximately 30% of genes located on the short arm of the X ...

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Drosophila X virus RNA polymerase: tentative model for in vitro replication of the double-stranded virion RNA.

Drosophila X virus RNA polymerase: tentative model for in vitro replication of the double-stranded virion RNA.

... If such was the case with drosophila X virus, then i the RNase sensitivity of the 32P-labeled molecules would be increased by the end of the reaction as compared with the nonincubated vi[r] ...

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Gene Expression During the Development of Bacillus subtilis Bacteriophage φ29 II. Resolution of Viral-Specific Ribonucleic Acid Molecules

Gene Expression During the Development of Bacillus subtilis Bacteriophage φ29 II. Resolution of Viral-Specific Ribonucleic Acid Molecules

... It should be noted that synthesis of the major early component, 0.75 x 106 daltons, appears to cease at approximately the time in infection when late RNA begins to be synthesized.. The c[r] ...

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Reversible Inhibition of Poliovirus RNA Synthesis In Vivo and In Vitro by Viral Products

Reversible Inhibition of Poliovirus RNA Synthesis In Vivo and In Vitro by Viral Products

... During the in vitro incorporation of [3HJUTP by viral RNA polymerase preparations in the presence of 750 x g supernatants, the following RNase inhibitors were added to the enzyme test: P[r] ...

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Allele specific analysis of cell fusion mediated pluripotent reprograming reveals distinct and predictive susceptibilities of human X linked genes to reactivation

Allele specific analysis of cell fusion mediated pluripotent reprograming reveals distinct and predictive susceptibilities of human X linked genes to reactivation

... to X chromo- some reactivation (XCR) and their location has not been achieved, mainly due to the variable outcomes of human iPSC reprograming and a paucity of studies that directly assess the expression of ...

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Synthesis, crystal structures and docking studies of 2,7-diphenyl-1,4-diazepan-5-one derivatives

Synthesis, crystal structures and docking studies of 2,7-diphenyl-1,4-diazepan-5-one derivatives

... In this study, two new crystal structures of 1,4-diazepine and its nitroso derivative(DIAZ1 & DIAZ2) were synthe- sized and characterized by X-ray crystallographic methods. Both 1,4-diazepine derivatives are ...

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Defects in dosage compensation impact global gene regulation in the mouse trophoblast

Defects in dosage compensation impact global gene regulation in the mouse trophoblast

... the X chromosome coated with Xist CAGΔ5′ RNA manifested histone modification patterns typical for the normally inactivated X chromosome in XX CAGΔ5′ , suggesting that Xist CAGΔ5′ RNA could ...

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Monocistronic and polycistronic bacteriophage T4 gene 23 messages.

Monocistronic and polycistronic bacteriophage T4 gene 23 messages.

... As in preparations of early RNA, gene 32 message activity was present in two species of RNA, which had molecular weights of approximately 0.5 x 106 and 0.8 x 106, and IPIII message activ[r] ...

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X-region-specific transcript in mammalian hepatitis B virus-infected liver.

X-region-specific transcript in mammalian hepatitis B virus-infected liver.

... Therefore, to demonstrate that the transcript was X-region specific, Northern blot hybridization was performed on nuclear RNA isolated from WC220 liver tissue using radiolabeled probes f[r] ...

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Chromosome silencing mechanisms in X chromosome inactivation: unknown unknowns

Chromosome silencing mechanisms in X chromosome inactivation: unknown unknowns

... Xist RNA (Kaneko et ...known RNA-binding ...Moreover, RNA immunoprecipitation (RIP) experiments aimed at verifying such interactions in vivo were carried out either in the absence of cross-linking ...

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