RPMI-1640 medium
Interlaboratory evaluation of Etest method for testing antifungal susceptibilities of pathogenic yeasts to five antifungal agents by using Casitone agar and solidified RPMI 1640 medium with 2% glucose
... the RPMI 1640 medium since Casi- tone agar contains compounds antagonistic to this antifungal ...per medium with each drug for each ...drug, medium, and incubation ...
5
Modulation of Morphogenesis in Candida albicans by Various Small Molecules
... M199 medium at 37°C (1, 50, 135, ...in RPMI 1640 medium at 37°C by reducing polyamine levels, decreasing CYR1 mRNA levels, and reducing cAMP cellular levels ...M199 medium at 37°C (47, ...
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Evaluation of in vitro culture systems for the maintenance of microfilariae and infective larvae of Loa loa
... containing RPMI 1640 medium (Sigma-Aldrich, St Louis, ...ml RPMI was slowly layered on the surface of a 15 ml tube con- taining stock iso-osmotic Percoll® and centrifuged (Humax 14k human, ...
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Cell mediated Immunity in Legionnaires' Disease
... Freshly explanted monocytes from a nonpatient were incubated in monolayer culture for 24 h at 37°C in 5% C02-95% air in 500 IAI RPMI 1640 medium containing 20% fresh nonpatient serum and[r] ...
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Attempted Isolation of Nanobacterium sp Microorganisms from Upper Urinary Tract Stones
... Cell coculture. Gimenez staining of inoculated 3T6 cells and on inoculated RPMI 1640 medium failed to reveal nanobacte- ria after 1 week of culture in any of the 10 stone samples (Table 1). However, ...
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Evaluation of the Anti-cancer Activity of most potent Ethanolic fraction of Prunus avium on EAC Cells in RPMI 1640
... followed by solvent evaporation by rotary vacuum evaporator. Most potent fraction was determined by fractionation by using Chloroform, n-butanol and ethyl acetate followed by qualitative and quantitative analysis. Most ...
6
DEVELOPMENT OF HERBAL FORMULATION WITH AJWA SEED (PHOENIX DACTYLIFERA L ) EXTRACT AND ZAM ZAM WATER FOR INVITRO ANTICANCER ACTIVITY
... in RPMI-1640 medium supplemented with FBS (fetal bovine serum), 100units/ml penicillin, 100μg/ml streptomycin, and were cultured in a humidified atmosphere of 5% CO2 and 95% air at ...in ...
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The Cytotoxicity of Silver Nanoparticles Coated with Different Proteins on Balb/c Macrophage Cells
... after medium protein adsorption [1, 2], there is no report on other proteins which may not be contained in ...in RPMI medium, the quantity of uptake by macrophages, the mechanisms of uptake, and the ...
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Transcriptional Profiling Identifies a Role for BrlA in the Response to Nitrogen Depletion and for StuA in the Regulation of Secondary Metabolite Clusters in Aspergillus fumigatus
... YPD medium to ensure that the cultures remained submerged and did not ...to RPMI 1640 medium (Sigma-Aldrich product number R6504) buffered at pH ...MOPS-buffered RPMI 1640 ...
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Detection of Resistance to Amphotericin B amongCryptococcus neoformans Clinical Isolates: Performances of Three Different Media Assessed by Using E Test and National Committee for Clinical Laboratory Standards M27 A Methodologies
... NCCLS-specified RPMI 1640 medium is re- placed with antibiotic medium 3 (also known as Penassay broth) (22, ...antibiotic medium 3 in combination with the E-test agar-based methodology ...
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Binding, uptake, and intracellular trafficking of phosphorothioate modified oligodeoxynucleotides
... In these studies, K562 cells 2 x 10' cells/ml suspended in RPMI 1640 with 2% bovine calf serum GIBCOBRL were incubated in the presence or absence of 100 nM c-myb antisense 'P-ODN at 4°C [r] ...
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GPR101 mediates the pro resolving actions of RvD5n 3 DPA in arthritis and infections
... on human macrophages. (A) Human monocyte–derived macrophages were incubated with either an siRNA against GPR101 or a control sequence (CT siRNA) for 96 hours, and GPR101 expression was assessed using flow cytometry (n = ...
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VER-246608, a novel pan-isoform ATP competitive inhibitor of pyruvate dehydrogenase kinase, disrupts Warburg metabolism and induces context-dependent cytostasis in cancer cells
... The following day the cells were treated with the indicated concentrations of VER-246608 and Nov3r in RPMI-1640 media containing 0.25 g/L D-glucose and 2% dialyzed FCS, followed by the r[r] ...
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Production, expansion and clonal analysis of T cells with specific HLA restricted male lysis.
... Oxnard, Cahf) kept at an angle of 45° in a humidified 5% CO 2 incubator The culture medrum was RPMI-1640 that was supplemented with 20 raM L-glutamine/ml (Flow Laboratories Lid , Irvine[r] ...
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Axitinib Targeted Cancer Stemlike Cells to Enhance Efficacy of Chemotherapeutic Drugs via Inhibiting the Drug Transport Function of ABCG2
... diphenyllapatinibrazolium bromide (MTT), vincristine, topotecan, mitoxantrone, Dox, rhodamine 123, verapamil, MK571 and FTC were products of Sigma Chemical Co (St. Louis, MO, USA). Axitinib was pur- chased from Selleck ...
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Nano-Se crosstalks with nano-DOX/FU to selectively hack hepatic cancer cells and spare normal cells healthy: A mechanism-based study
... All drug groups were evaluated by MTT assay using HepG2/BHk-21 cells. Briefly, the cells were cultured in 96-well plates at a density of 5×10 3 cells/well. All drugs with their described concentrations were added in ...
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Influence of Glucose Supplementation and Inoculum Size on Growth Kinetics and Antifungal Susceptibility Testing ofCandida spp
... The influences of inoculum size and glucose supplementation on the growth kinetics of 60 Candida spp. clinical isolates (Candida albicans, Candida tropicalis, Candida parapsilosis, Candida glabrata, Candida krusei, and ...
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Niclosamide enhances abiraterone treatment via inhibition of androgen receptor variants in castration resistant prostate cancer
... 12 months of being cultured in abiraterone acetate containing medium, C4-2B AbiR (C4-2B abiraterone resistant) cells developed resistance to abiraterone acetate. Abiraterone acetate at 5 μ M significantly ...
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Molecular Identification of Agents of Human Cutaneous Leishmaniasis and Canine Visceral Leishmaniasis in Different Areas of Iran Using Internal Transcribed Spacer 1 PCR-RFLP
... Samples were collected from 148 lesion exudates of suspected patients to CL referred to Leishmaniasis Laboratory of the School of Public Health, Tehran University of Medical Sciences, Tehran, Iran, and District Health ...
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Plasmodium vivax: paroxysm associated lipids mediate leukocyte aggregation
... of RPMI 1640 [1] in a 24-well tissue culture plate (Flow Lab- oratories, UK) and 200 μ l of test (paroxysm plasma, pre- paroxysm plasma, post-paroxysm plasma, non-malarial fever plasma or plasma from ...
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