Scheme for seeding cells into a microfluidic device
A Microfluidic Device for Ultrasonic Separation
... of device As in the Cardiff laminar flow filter [2], a fluid/particle mixture is drawn into the device through the port on the left of the ...a scheme allows for a relatively small quantity of ...
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Phase separation of multiphase droplets in a digital microfluidic device
... the amount of water residue to IL is similar to IL residue to water in SL1. In summary, we have Fig. 10 to understand the effects of different schemes on residue formation. Each column represents the average value of the ...
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Droplet-Based Microfluidic Scheme for Complex Chemical Reactions
... The monodisperse silica particles are allowed to travel within the Teflon tube for about 20 min. Subsequently, they are collected outside of the microfluidic device in a beaker containing the same ...
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A droplet-based microfluidic scheme for complex chemical reactions
... droplet-based microfluidic reaction scheme explicitly by producing monodisperse silica particles using sol-gel ...via microfluidic sol-gel synthesis scheme are further extended to produce ...
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Efficient generation of hepatic cells from mesenchymal stromal cells by an innovative bio-microfluidic cell culture device
... stromal cells (MSCs) are multipotent and have great potential in cell ...these cells can rescue fulminant hepatic ...lineage cells from MSCs required to improve, but also the need for a large number ...
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Bioluminescent response of individual dinoflagellate cells to hydrodynamic stress measured with millisecond resolution in a microfluidic device
... the microfluidic device to previous flow experiments To connect the results of this study with previous work on dinoflagellate bioluminescence in fully developed pipe flows, it is instructive to compare the ...
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Mutational Analysis of Circulating Tumor Cells Using a Novel Microfluidic Collection Device and qPCR Assay
... target cells of a specific pathol- ogy. The sample passes through a microfluidic device that contains an isolation zone to capture CTCs on the upper surface of the cartridge in an externally applied ...
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The Study of Pancreatic Circulating Tumor Cells via Isolation with an Integrated Microfluidic Immunomagnetic (IMI) Device
... categorized cells as having low, moderate and high EpCAM expression among levels which can potentially reflect different physiological contributions of each cell subset to pancreatic ...of cells such as the ...
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High-Purity Isolation and Recovery of Circulating Tumor Cells using Conducting Polymer-deposited Microfluidic Device
... released cells by visual- izing them with cell-specific markers, such as an- ti-cytokeratin (anti-CK) for the identification of epi- thelial cells, Hoechst dye for nuclei, and anti-CD45 for haematologic ...
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Study of the chemotactic response of multicellular spheroids in a microfluidic device
... a microfluidic device to observe chemotactic migration in multicellular ...A microfluidic device was designed comprising a central micro- chamber and two lateral channels through which ...
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Design and validation of a microfluidic device for controlled cell stimulation
... Synopsis Microfluidic devices promise to enhance fundamental biological research by offering precise, consistent, and easily adjustable experimental ...to cells with high spatiotemporal precision will allow ...
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Isolation and detection of circulating tumour cells from metastatic melanoma patients using a slanted spiral microfluidic device
... incubation, cells were washed three times with ...Finally, cells were mounted using ProLong® Gold Antifade Mountant with DAPI (4',6-diamidino-2-phenylindole) nuclear stain reagent (Thermo Fisher ...Stained ...
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Separating mouse malignant cell line (EL4) from neonate spermatogonial stem cells utilizing microfluidic device in vitro
... the cells larger than 12 μm and cells smaller than that flow into the outer ...these cells will separate by inner and outer outlets. The collected cells can then be ana- lyzed by suitable ...
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A microfluidic device to study cancer metastasis under chronic and intermittent hypoxia
... The device reported herein will be used to systematically study intermittent hypoxia in can- cer cell ...our device allows any of these cycling profiles to be ...cancer cells feed substrates to ...
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A microfluidic device to acquire high-magnification microphotographs of yeast cells
... the microfluidic chip was undesirably changed. Because cells were pushed up by the PDMS membrane with air pressure from the control channel while acquiring images, the cells were possibly flattened ...
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A Microfluidic Device for Thermal Flow Cytometry.
... The device resistance forms R LOAD shown in Fig 3.14. The circuit was operated with inputs of +15 V and -15V supplied from an Agilent E3649A power source. The heating electrodes of the device were connected ...
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Design for Microfluidic Device Manufacture Guidelines
... UV Nanoimprint is designed as massive parallel replication process of sacrificial layers or permanent layers. Etch selectivity is comparable to standard semiconductor processes but needs to be adjusted for the underlying ...
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Microfluidic device for primary tumor spheroid isolation
... trapping device has been reported in [22] in which spheroids generated from an established cancer cell line were captured within a trap formed by PDMS posts within the ...This device has the added capacity ...
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Integrated DNA extraction and amplification on a microfluidic device
... the microfluidic device was demonstrated in Chapter ...individual microfluidic devices in a research setting, tubing can be held securely in position on the microfluidic device by ...
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Design and Verification of a Blood Cell Separation Microfluidic Device
... particular cells from blood, such as cancer ...flow microfluidic device is controlled by several ...These cells are subject to different types of forces that result from nat- ural or external ...
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