Schizosaccharomyces pombe
Suppression of Meiotic Recombination by CENP-B Homologs in Schizosaccharomyces pombe
... ABSTRACT Meiotic homologous recombination (HR) is not uniform across eukaryotic genomes, creating regions of HR hot- and coldspots. Previous study reveals that the Spo11 homolog Rec12 responsible for initiation of ...
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A conserved histone deacetylase with a role in the regulation of cytokinesis in Schizosaccharomyces pombe
... All Schizosaccharomyces pombe strains used in this study are listed in Table ...S. pombe cells were cultured in YES or in Edinburgh Minimal Media (EMM) supplemented with adenine, histi- dine, ...
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Conserved Locus-Specific Silencing Functions of Schizosaccharomyces pombe sir2+
... In Schizosaccharomyces pombe, three genes, sir2 ⫹ , hst2 ⫹ , and hst4 ⫹ , encode members of the Sir2 family of conserved NAD ⫹ -dependent protein ...S. pombe sir2 ⫹ gene encodes a nuclear protein ...
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A Novel Gene, msa1, Inhibits Sexual Differentiation in Schizosaccharomyces pombe
... Sexual differentiation in the fission yeast Schizosaccharomyces pombe is triggered by nutrient starvation or by the presence of mating pheromones. We identified a novel gene, msa1, which encodes a 533-aa ...
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Characterization of rec7, an Early Meiotic Recombination Gene in Schizosaccharomyces pombe
... rec7 is involved in intra- and intergenic meiotic recombination in all tested regions of the genome of the fission yeast Schizosaccharomyces pombe. Segregational analysis in a rec7 gene disruption mutant ...
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Characterization of Functional Regions in the Schizosaccharomyces pombe mei3 Developmental Activator
... The Schizosaccharomyces pombe mei3 1 gene is expressed only in diploid cells undergoing meiosis. Ectopic expression of mei3 1 in haploid cells causes meiotic catastrophe. Mei3 is an inhibitor of Ran1/Pat1 ...
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The Schizosaccharomyces pombe cho1+ Gene Encodes a Phospholipid Methyltransferase
... of Schizosaccharomyces pombe defective in the synthesis of phosphatidylcholine via the methylation of phosphatidylethanolamine is ...S. pombe to be cloned and ...S. pombe cho1 ...
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The Meiotic Recombination Hot Spot ura4A in Schizosaccharomyces pombe
... The meiotic recombination hot spot ura4A (formerly ura4-aim) of Schizosaccharomyces pombe was observed at the insertion of the ura4 ⫹ gene 15 kb centromere-proximal to ade6 on chromosome III. Crosses ...
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Characterisation of a Holliday junction resolving enzyme from Schizosaccharomyces pombe
... With the exception of CCE1, very little is known about the enzymology of homologous recombination in eukaryotes. Although junction-resolving activities have been detected in S. cerevisiae (40), mammalian cells (8, 14), ...
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Kinetochore assembly and heterochromatin formation occur autonomously in Schizosaccharomyces pombe
... yeast Schizosaccharomyces pombe is a key system for the experimental study of centromere ...S. pombe requires investigation using new ...S. pombe showed that this fission yeast is ...
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ALLELIC COMPLEMENTATION AT THE ADENYLOSUCCINASE LOCUS IN SCHIZOSACCHAROMYCES POMBE
... I t is hoped that future comparative studies of mutants at the ad-8 locus in Schizosaccharomyces pombe and at the ad-4 locus in Neurospora crassa will pro- vide more[r] ...
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The Spontaneous Mutation Rate in the Fission Yeast Schizosaccharomyces pombe
... yeast Schizosaccharomyces pombe, a key model organism with many similarities to higher ...S. pombe, generating 422 single-base substitutions and 119 insertion-deletion mutations (indels) across the ...
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Optimizing the Nucleotide Sequence of a Meiotic Recombination Hotspot in Schizosaccharomyces pombe
... The ade6-M26 mutation of Schizosaccharomyces pombe created a meiotic recombination hotspot. Previous analyses indicated that the heptamer 5⬘-ATGACGT-3⬘ was necessary and sufficient for hotspot activity; the ...
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Dynein Promotes Achiasmate Segregation in Schizosaccharomyces pombe
... Most organisms use crossovers (chiasmata) to maintain physical connections between homologous chromo- somes that ensure their proper segregation at the first meiotic division. The fission yeast Schizosaccharomyces ...
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Response of Schizosaccharomyces pombe to Zinc Deficiency
... A component of the cellular response to zinc deficiency operates via control of transcript abundance. Therefore, microarray analysis was employed to identify Schizosaccharomyces pombe genes whose mRNA ...
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Functional Genomics of Adhesion, Invasion, and Mycelial Formation in Schizosaccharomyces pombe
... yeast Schizosaccharomyces pombe was shown to form invasive filaments after nitrogen limitation ...S. pombe, on rich media. Screening of an S. pombe haploid deletion library identified 12 genes ...
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Active and inactive transplacement of the M26 recombination hotspot in Schizosaccharomyces pombe.
... We considered two technical explanations for the lack of M26 hotspot activity in the plasmid X chromo- some recombination assays: the multicopy plasmid con- taining the M[r] ...
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Characterization of Glycoside Hydrolase Family 5 Proteins in Schizosaccharomyces pombe
... S. pombe Exg1 and Exg3 were highly specific for  (1,6)-glucans (pustulan), being unable to degrade linear or branched  ...S. pombe Exg1 is similar to that previously suggested for the ...S. pombe ...
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Regulation of Leucine Uptake by tor1+ in Schizosaccharomyces pombe Is Sensitive to Rapamycin
... TOR signaling and nutrient uptake mechanisms: Stud- plex is required for PI3K/mTOR signaling, possibly by ies in S. cerevisiae and mammalian cells suggest that TOR repressing a negative feedback loop from mTOR/S6K ...
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Isolation and characterization of Schizosaccharomyces pombe mutants affected in mitotic recombination.
... pombe to screen for recombination deficient mutants directly: GOLDMANN and GUTZ (1 974) used a diploid strain heteroallelic for the ade6 gene and isolated the mutant strain r[r] ...
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