Sequencing depth of DNA samples from cultured
The impact of sequencing depth on the inferred taxonomic composition and AMR gene content of metagenomic samples
... to sequencing depth than AMR gene ...thermophilus DNA substantially changed the estimated gene abundance ...content from cultured isolates from effluent was recoverable using ...
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The impact of sequencing depth on the inferred taxonomic composition and AMR gene content of metagenomic samples
... to sequencing depth than AMR gene ...thermophilus DNA substantially changed the estimated gene abundance ...content from cultured isolates from effluent was recoverable using ...
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Preparing Samples for Sequencing Genomic DNA
... Illumina, Inc. does not assume any liability arising out of the application or use of any products, component parts, or software described herein. Illumina, Inc. further does not convey any license under its patent, ...
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Fungal DNA sequencing from laser capture microdissection samples
... Cycle Sequencing Kit can be successfully used to obtain DNA sequence information from the D1-D2 and ITS2 regions of fungal samples prepared from FFPE specimens using the Arcturus XT TM ...
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Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples
... article bau061. Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W, Bolchacova E, Voigt K, Crous PW, Miller AN, Wingfield MJ, Aime MC, An KD, Bai FY, Barreto RW, Begerow D, Bergeron MJ, Blackwell ...
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The Next Generation of Molecular Markers From Massively Parallel Sequencing of Pooled DNA Samples
... separate sequencing of individuals, both for estimating allele frequencies and for inference of population genetic ...When sequencing er- rors are not too common, pooling seems also to be a good choice for ...
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PacBio amplicon sequencing for metabarcoding of mixed DNA samples from lichen herbarium specimens.
... required from herbaria and living fungal collections for the mass-barcoding of well-identified and well-curated fungal specimens or ...amplicon sequencing approach is applied to recent lichen herbarium ...
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A comparison of ARMS and DNA sequencing for mutation analysis in clinical biopsy samples
... line DNA admixtures containing the mutation of interest in a normal DNA background (ranging from 100% mutant - 1% mutant in a normal background) was amplified in the same machine runs to act as ...
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ddpcr allows 16S rrna gene amplicon sequencing of very small DNA amounts from low-biomass samples
... for samples conducted using less than 50 pg initial DNA, the iso- lated amplicons were re-amplified in a standard PCR but using a Q5U polymerase with primer P5 and ...“rescue” samples which otherwise ...
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Hybrid selection for sequencing pathogen genomes from clinical samples
... genomes from clinical samples at scale due to the gross excess of host DNA typically ...host DNA contamina- tion is to augment sequencing coverage ...genome from pure template ...
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DNA Sequencing Handbook
... Automated DNA sequencing on capillary format instruments requires that the template be of higher purity than manual ...your samples carefully. Inadequate DNA cleanup is the most common cause ...
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Evolution of DNA Sequencing
... allows DNA fragments separation based on their ...rapid DNA fragment separation, it is the ease of automation that has resulted in its induction in sequencing instruments, in place of slab gel ...
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DNA capture and next-generation sequencing can recover whole mitochondrial genomes from highly degraded samples for human identification
... Nuclear DNA short tandem repeat (STR) profiling is currently the preferred method for human identification in forensic practice ...degraded DNA from trace sources or poorly preserved human remains is ...
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Direct 16S rRNA Gene Sequencing from Clinical Specimens, with Special Focus on Polybacterial Samples and Interpretation of Mixed DNA Chromatograms
... by DNA sequencing of the different ...in samples with large differences in the relative concentrations of the different par- ...different DNA fragments based on their physical ...different ...
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DNA Sequencing by Hybridization and Shotgun Sequencing Technique
... random DNA sequence of length 20,000 ...mean depth of coverage of 7 using the enzyme program of the GenFrag utility (Engle and Burks, 1993), version ...1994). Sequencing errors were simulated on this ...
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Whole genome enrichment and sequencing of Chlamydia trachomatisdirectly from clinical samples
... subjects from whom we obtained samples had evidence of resistant ...gene from sample CT-36, which have been associated with macrolide resistance when in com- bination with two mutations in the 23S ...
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Both Human Immunodeficiency Virus Cellular DNA Sequencing and Plasma RNA Sequencing Are Useful for Detection of Drug Resistance Mutations in Blood Samples from Antiretroviral Drug Naive Patients
... cross-sectional sequencing of the bulk PCR products obtained from plasma and PBMCs does not provide a detailed view of the natural history of primary ...PBMC DNA analysis in conjunction with the ...
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Review on DNA damage, repair and DNA sequencing
... Molecular biology – studying the genome itself, how proteins are made, what proteins are made, identifying new genes and associations with diseases and phenotypes, and identifying potential drug targets Evolutionary ...
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DNA from Dust: Comparative Genomics of Large DNA Viruses in Field Surveillance Samples
... comes from laboratory ...directly from field samples without the need for sequence-based enrichment strategies prior to ...genomes from the field, without prior culture. These viruses were ...
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Extraction of DNA from Difficult Samples with an Automatable Portable System
... for cultured bacteria and for extraction in reaction tubes, the extraction protocol was altered to suit a microchip system: First, most of the volumes were scaled down to one-fourth of the original ...
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