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PRESUMPTIVE TESTS FOR CYSTIC FIBROSIS BASED ON SERUM PROTEIN IN MECONIUM

PRESUMPTIVE TESTS FOR CYSTIC FIBROSIS BASED ON SERUM PROTEIN IN MECONIUM

PRESUMPTIVE TESTS FOR CYSTIC FIBROSIS BASED ON SERUM PROTEIN. Services[r]

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Change of serum prealbumin levels and serum protein markers between egg white powder and casein protein additives in standard enteral feeding formulas in critically ill patients with acute respiratory failure

Change of serum prealbumin levels and serum protein markers between egg white powder and casein protein additives in standard enteral feeding formulas in critically ill patients with acute respiratory failure

In developing countries, protein supplements are lim- ited in supply and they are costly. Casein protein addi- tive was used regularly in our unit as a standard protein additive for a period of time, but the availability was lim- ited and the cost was high. For this reason, protein sup- plementation in critically ill patients was possibly inadequate. Egg white protein powder, which is normally used in the food industry, is widely available, is less costly, and possibly gives a similar BV and PER to casein protein. As a result, this type of protein additive has been suggested as a substitute for casein protein additive in our unit. This study was conducted to determine the effect of serum protein marker change between egg white protein additive and casein additive in critically ill patients by the non-inferiority fashion.
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EFFECT OF DIET DURING THE FIRST SIX TO EIGHT WEEKS OF LIFE ON TOTAL CONCENTRATIONS OF SERUM PROTEIN AND ON ELECTROPHORETIC PATTERNS OF SERUM PROTEIN AND LIPOPROTEIN

EFFECT OF DIET DURING THE FIRST SIX TO EIGHT WEEKS OF LIFE ON TOTAL CONCENTRATIONS OF SERUM PROTEIN AND ON ELECTROPHORETIC PATTERNS OF SERUM PROTEIN AND LIPOPROTEIN

Comparison of the mean values for total protein concentrations in serum found in this study with those reported in the litera ture reveals (a) concentrations in maternal sera which range[r]

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THYROXINE BINDING BY SERUM PROTEIN IN PREGNANCY AND IN THE NEWBORN

THYROXINE BINDING BY SERUM PROTEIN IN PREGNANCY AND IN THE NEWBORN

This finding is also con- roxine in the opposite directions In a pregnancy sistent with a low free thyroxine concentration serum with 1.4 times 10-7 M thyroxine and 5.1 and a relatively [r]

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Corrected fructosamine, corrected glycated albumin and glycated β-lipoprotein/ β-lipoprotein ratio in patients with microvascular complications of type 2 diabetes mellitus

Corrected fructosamine, corrected glycated albumin and glycated β-lipoprotein/ β-lipoprotein ratio in patients with microvascular complications of type 2 diabetes mellitus

method, urinary protein was measured by Pyrogallol red end point method and urinary protein/creatinine ratio was calculated. All biochemical investigations were performed on a fully automated analyzer. Serum glycated β-lipoprotein was measured by agarose gel film electrophoresis at pH 8.6 followed by colour development with nitro-blue tetrazolium (NBT) by a modification of staining method adapted from Kunio Kobayashi et al [4]. Dried and compressed electrophoresis plate was immersed for 20 hours in glycated protein colour development reagent (5 mmol/l NBT in 50 mmol/l sodium carbonate buffer pH 10.3) at room temperature followed by two washes of stop solution (0.3% citric acid). NBT stained blue bands on the glycated protein film were scanned at 540 nm (Olympus glass filter plate) using a densitometric scanner (Nikon Super COOLSCAN 9000 ED). Image was analysed by free electrophoresis image analysis software GelAnalyzer 2010a. Proportions (%) of glycated albumin and glycated lipoprotein were represented as the percentage of total area under the scanned profile attributable to each peak. Concentration (in µmol/l) was calculated by multiplying percentage with serum fructosamine value. Staining in β region was considered to represent glycation of β- lipoproteins. Serum protein (stained with 0.5% acid blue 29 in 5% acetic acid) and lipoprotein (stained with 0.05% fat red 7B and 0.05% oil red O in methanol: water, 4:1) electrophoresis was performed simultaneously for all the samples to aid in quantitation by comparison of mobility of respective proteins (figure 1).
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3.	Mays T. Abdalla, Mohammed B. Hassan and Suhban S. Al-Mallah

3. Mays T. Abdalla, Mohammed B. Hassan and Suhban S. Al-Mallah

inflammation and have higher levels of inflammatory mediators which are now considered as one of the emerging and important factors that are associated with the alteration in the thyroid function in patients with chronic renal failure (Enia et al., 2007). Although higher frequency of low T3 had been observed in patients with longer duration of hemodialysis and CRF, but no statistically significant association had been detected between longer duration and lower serum T3 levels. This finding is similar to what had been concluded from Pagliacci et al. (1987) study. They found that there is no association between starting the replacement therapy and its duration and having low serum T3 levels, with same tendency toward lower T3 values with longer duration. This tendency can be explained by the lower Glomerular filtration rate that might be present in patients with CRF for longer period. In addition, this insignificant association can be due to small sample size that this study had include, and larger sample size is required to clarify the effect of CRF duration on thyroid function test. Regarding the relationship between the results of thyroid function tests in patients with CRF and hemodialysis and total serum protein and serum albumin, this study had showed that despite the finding of modestly higher means which had been observed in patients with higher values of serum albumin and total serum protein, but the only statistically significant association had been observed between the mean serum T3 level and serum TSH in one hand and serum albumin and total serum protein in other hand, respectively. This finding of no significant association can be related to the small sample size as it had been mentioned earlier. Furthermore, although circulating thyroid hormones binding globuline and albumin levels are typically normal in uremia (in the absence of nephrotic syndrome), retained substances may inhibit hormone binding to these proteins. The interesting point to be mentioned here is that hemodialysis patients some transient elevation in serum T4 levels commonly occurs during hemodialysis, Herchman et al. (1972) found that this phenomenon is related to the use of heparin as a routine maneuver to prevent clotting in
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Biomarker changes in Diabetic rats Treated with Ethanolic Plant Extract of Passiflora Foetida Linn.

Biomarker changes in Diabetic rats Treated with Ethanolic Plant Extract of Passiflora Foetida Linn.

The level of total protein and liver marker enzymes such as SGPT, SGOT and ALP in the serum of diabetic rats are presented in Table 2. Significant reductions in serum protein was observed in induced diabetic rats (Group II) when compared to control rats (Group I). On administration of ethanol extract of P.foetida leaf to the diabetic rats, protein level were found to be restored in normal. Also, the SGPT, SGOT and ALP levels were elevated significantly in induced diabetic rats compared to control rats. Both the doses of P.foetida leaf extracts and glipizide treatment significantly reduced above parameters compared to diabetic control rats
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Original Article Application of SELDI-TOF-MS in screening serum biomarkers of newly diagnosed immune thrombocytopenic purpura

Original Article Application of SELDI-TOF-MS in screening serum biomarkers of newly diagnosed immune thrombocytopenic purpura

Abstract: Objective: To investigate the surface-enhanced laser desorption/ionization-time of flight (SELDI-TOF-MS) method in screening the serum protein markers for the early diagnosis, disease monitoring, and prognosis determi- nation of newly diagnosed immune thrombocytopenic purpura (NITP), and to establish a new molecular diagnostic method of NITP. Methods: SELDI-TOF-MS was used to detect the serum proteins binding on the protein chips so as to obtain the serum protein expression fingerprints of the NITP and healthy controls, respectively, and then Biomarker Wizard and Biomarker Paterns System 5.0 software were used to analyze the data and to establish the artificial neural network diagnostic model (ANNDM). Results: M/E was within 2000 to 20000, and the most significant proteins were those with M/E as 4109.23 and 5521.35, respectively, which can be seen as the serum biomarkers of NITP; the corresponding proteins were then searched in the protein library; the serum markers were then input into ANNDM to accurately group the children with NITP from the healthy children. Conclusions: The serum protein expression fingerprint has important clinical significance toward the early diagnosis of NITP, as well as in exploring the pathogenesis of NITP. The diagnostic model composed of the proteins with M/E as 4109.23 and 5521.35 can completely and accurately distinguish the children with NITP from healthy children.
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Activity of florfenicol for Actinobacillus pleuropneumoniae and Pasteurella multocida using standardised versus non-standardised methodology

Activity of florfenicol for Actinobacillus pleuropneumoniae and Pasteurella multocida using standardised versus non-standardised methodology

for florfenicol; after correction for binding to serum protein, all three indices of potency were 420. significantly lower in serum than in broth, indicating that correction for protein[r]

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Objective Evaluation of Patients with Rheumatic Diseases  II  Paper Electrophoretic Studies of Serum Glycoprotein and Protein from Patients with Rheumatoid Arthritis

Objective Evaluation of Patients with Rheumatic Diseases II Paper Electrophoretic Studies of Serum Glycoprotein and Protein from Patients with Rheumatoid Arthritis

Total serum glycoprotein expressed as hexose polysaccharide as a percentage of the serum protein exhibited the highest correlation with clinical activity, and consequently is to be recom[r]

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 HEPATOPROTECTIVE EFFICACY OF CUCUMIS CALLOSUS (ROTTL.) COGN. (CUCURBITACEAE) FRUIT AGAINST CCL4-INDUCED HEPATIC DAMAGE IN RATS

 HEPATOPROTECTIVE EFFICACY OF CUCUMIS CALLOSUS (ROTTL.) COGN. (CUCURBITACEAE) FRUIT AGAINST CCL4-INDUCED HEPATIC DAMAGE IN RATS

the experimental rats for 16 days. The hepatoprotective effect of MECC was evaluated by the assay of serum biochemical parameters viz. serum glutamine oxaloacetate transaminase (SGOT), serum glutamine pyruvate transaminase (SGPT), serum alkaline phosphatase (SALP), total serum protein, total bilirubin content; and liver biochemical parameters such as thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) content, superoxide dismutase (SOD), catalase (CAT) and histopathological studies of the liver. In methanol extract-treated animals, the toxic effect of CCl 4 was controlled
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Disturbance of Serum Viscosity in Diabetes Mellitus

Disturbance of Serum Viscosity in Diabetes Mellitus

Intrinsic viscosity, abbreviated [h], is a concentration-independent solute property related to molecular shape. [h] was found to be 7% higher in diabetic than in normal serum. The [h] difference accounted for at least half of the serum viscosity elevation. The rest of the increase was due to increased serum protein level and increased nonprotein solids, presumably glucose and lipid. Associated with increased [h] was a decline in albumin: globulin ratio and elevation of the acute phase reactant proteins, a 1 -acid glycoprotein, a 1 - antitrypsin, haptoglobin, and ceruloplasmin. Studies comparing diabetic and normal serum fractionated by using 21.5% sodium sulfate showed that changes in [h] were attributable to changes in serum protein composition rather than an inherent qualitative disturbance of protein […]
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oa Journal of the Egyptian National Cancer Institute - Hepatocellular functions after repeated exposure to halothane in women: undergoing radium therapy for cervical cancer (a clinical study)

oa Journal of the Egyptian National Cancer Institute - Hepatocellular functions after repeated exposure to halothane in women: undergoing radium therapy for cervical cancer (a clinical study)

A clinical study was carried on 50 women having cervical carcinom a and who were subjected to general anaesthesia for exam ination, cystoscopy to confirm diagnosis, staging and further management. The anaesthesia consisted o f I.V. induction and NzO : 0 2 H alothane for m aintenance. Changes in some liver function tests nam ely total serum protein (TSB), Serum album in, and globulin, A f G ratio, serum alkaline phosphatase (SAP), serum lactic dehydrogenase (SLD), and serum asparate am inotrasferose (SGOT) were m onitored and assessed in preoperative and postoperative periods. The results o f the stu d y revaeled the relative safety o f halothane anaesthesia in those patients as no significant changes were bserved in any o f the liver function tests assessed except a trend tow ards rise in TSB after the 2nd and 3rd exposures th at was too slight to produce clinical jaundice.
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NEWS AND ANNOUNCEMENTS

NEWS AND ANNOUNCEMENTS

the extracellular serum protein concentration in the nephrotic syndrome, although in this situa- tion, the increased protein concentration might be the result of extravasation of serum t[r]

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Studies relating to the interactions of proteins with hormones, metabolites and drugs in biological systems

Studies relating to the interactions of proteins with hormones, metabolites and drugs in biological systems

Serum La.boratoriea for donation of serum protein fractions;.. to the many people who.[r]

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<p>A Single-Center Retrospective Study to Investigate the Follow-Up of Patients with Monoclonal Proteinemia by Community Physicians in the UK</p>

<p>A Single-Center Retrospective Study to Investigate the Follow-Up of Patients with Monoclonal Proteinemia by Community Physicians in the UK</p>

Studies in Olmsted County have reported the occur- rence of axial fractures is signi fi cantly increased in MGUS even in the absence of progression to MM. 7 Other studies have shown that MGUS is a risk factor for fracture. 8–10 Studies have suggested that MGUS is associated with increased risk of arterial and venous thrombosis. 11–13 MGUS can cause monoclonal gammopathy of renal sig- ni fi cance, a spectrum of renal disease that includes AL amyloidosis and proliferative glomerulonephritis with monoclonal immunoglobulin (Ig) deposits. 14 MGUS can be associated with peripheral neuropathy. 15 The manage- ment of these B-cell related disorders may need early intervention and a new concept of monoclonal gammopa- thy of clinical signi fi cance (MGCS) has been suggested. 16 Reported rates of progression of MGUS to myeloma vary and there are a limited number of studies on the risk of MGUS progression to MM in select population groups. Further, MGUS patients appear to undergo inadequate work-up, follow-up and treatment in a community setting. 17 Guidelines suggest that serum protein electro- phoresis (SPEP) should be performed if there is clinical suspicion of an M-protein-related disorder, raised total protein/globulin or immunoglobulins, particularly if one or more immunoglobulin classes (IgG, IgA, IgM) are reduced. 18
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LINKAGE AMONG CATTLE BLOOD AND MILK POLYMORPHISMS

LINKAGE AMONG CATTLE BLOOD AND MILK POLYMORPHISMS

Sequential probability linkage tests of 88 cattle blood group, serum protein, and milk protein locus combinations.. 403.[r]

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Computation of In Vivo Antidiabetic Activity of Holarrhena Antidysenterica Seeds Extracts in Streptozotocin-Induced Diabetic Rats

Computation of In Vivo Antidiabetic Activity of Holarrhena Antidysenterica Seeds Extracts in Streptozotocin-Induced Diabetic Rats

Medicinal plants have curative properties due to the presence of various complex chemical compositions, which are found as secondary plant metabolites in one or more parts of the plant. The aim of this study was to compute the antidiabetic activity of Holarrhena antidysenterica seeds extract in streptozotocin- induced diabetic rats. The experimental protocol designed as animals were divided into six groups (n=6) as control, diabetic control, Glibenclamide, methanol extract (MEHAD), petroleum ether extract (PEHAD) and aqueous extract (AEHAD). Except control group, other remaining groups were treated with streptozotocin (STZ) (35 mg/kg body weight) by single i.v. injection to induce diabetes. The diabetic rats were treated with the glibenclamide, MEHAD (250 mg/kg body weight), PEHAD (250 mg/kg body weight) and AEHAD (250 mg/kg body weight) for 18 days. The fasting plasma glucose level, body weight, fasting serum glucose level, serum cholesterol, serum triglyceride, total protein, blood urea, urine glucose and liver glycogen levels were determined. The diabetic rats treated with MEHAD, PEHAD and AEHAD showed significant reduction in fasting serum glucose, serum cholesterol, serum triglyceride, total protein, blood urea, urine glucose and protection from the loss of body weight and increase in liver glycogen content during the treatment period. These effects were comparable to those seen in the glibenclamide- treated group of rats. This suggests that the Holarrhena antidysenterica seed extracts posses antidiabetic activity and further studies are needed to elucidate the mechanism of action and to know the active principles involved in producing the effect.
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Anti carbamylated protein antibodies precede disease onset in monkeys with collagen induced arthritis

Anti carbamylated protein antibodies precede disease onset in monkeys with collagen induced arthritis

RF-IgM antibodies were also measured with an in- house ELISA, using rabbit IgG (Sigma) as the antigen, which was diluted to 10 μg/ml in carbonate buffer, pH 9.6 and incubated overnight at room temperature. Plates were blocked for 1 hour at 37 °C with PBS, 1% BSA. Serum samples were diluted × 100 in PBS, 1% BSA and 0.05% Tween and incubated for 1 hour at 37 °C. Antibody binding was detected with goat-anti-human- IgM-HRP (Life technologies, 627520). Cross-reactivity of this antibody was confirmed by measuring anti-collagen IgM antibodies in a selection of the monkey serum sam- ples. All washing steps were carried out with PBS, 0.05% Tween. 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulpho nic acid) (ABTS) was used for final detection.
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Hemodynamic, Thyroid and Immunomodulatory Effects of Heroin in Rats

Hemodynamic, Thyroid and Immunomodulatory Effects of Heroin in Rats

Ca+2 concentrations of diacetylmorphine addicts shows a significant decrease compared to that of the control group (Li, et al., 2011). In our finding, however, serum NO level was significantly reduced by heroin administration as compared with control group (Fig. 3). (Habibey, et al., 2010) also resulted that morphine significantly could decrease plasma NO levels in rats. Such results may be due to the compensatory mechanism for decreased BP. Also, (Rezazadeh, et al., 2014) resulted that morphine significantly attenuate systolic blood pressure, diastolic blood pressure, and mean arterial pressure in the 2K1C animals; they also showed that serum concentrations of nitric oxide were decreased. It has also been suggested that intravenous morphine increases release of NO from spinal cord by α - adrenergic and cholinergic mechanism (Xu, et al., 1997). Another possible mechanism for antihypertensive effects of heroin may be associated with sodium homeostasis, because there is now well established that sodium ions has a major role for depolarizing membranes, hence producing vasoconstriction, sympathetic activation and hypertension (Barrett, et al., 2010). Although, little is known about the effect of diacetylmorphine on serum sodium, but according to the present result shown in Table II, serum urea as indicator for renal functions was significantly elevated and this is may be due its enhancement of kidney injury (Dettmeyer, et al., 2005) reporting that heroin associated with nephropathy. Although, recent studies observed that heroin significantly increase creatinine clearance (Javadian, et al., 2013). The present results also showed that diacetylmorphine
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