single-cell transcriptomics
Single Cell Transcriptomics Reveal a Correlation between Genome Architecture and Gene Family Evolution in Ciliates
... (i) single-cell transcriptomics provides numerous insights into genome evolution ...generated single-cell transcriptomic analyses of uncultivable ciliates (Table 1) and deposited the ...
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Single-cell transcriptomics reveal that PD-1 mediates immune tolerance by regulating proliferation of regulatory T cells
... in single-cell transcriptomics offer a new opportunity to discover additional subsets and cellular states of immune cells during development and disease pathogenesis at high resolution ...no ...
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Single cell transcriptomics suggest that human adipocyte progenitor cells constitute a homogeneous cell population
... unbiased single cell transcriptomics we aimed to characterize ASC populations in human subcutaneous white adipose tissue ...574 single cells from the WAT total stroma vascular fraction (SVF) ...
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In silico lineage tracing through single cell transcriptomics identifies a neural stem cell population in planarians
... different cell types and that gene expression becomes more specific as cells ...each cell with the number of sequencing reads, which revealed no correlation (Pearson correlation = ...non-stem cell ...
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Bulk and single-cell transcriptomics identify tobacco-use disparity in lung gene expression of ACE2, the receptor of 2019-nCov
... GSE122960 dataset was generated from lung tissue of 8 lung transplant donors (including 1 Asian former smoker, 2 Caucasian current smoker and 5 African American non-smokers), using 3' V2 chemistry kit on 10x Genomics ...
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Single-cell transcriptomics–based MacSpectrum reveals macrophage activation signatures in diseases
... define cell lineages, signals altered during macrophage terminal maturation and activation are relatively less distinct, as we observed in this study and others have pointed out as one of the major computational ...
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Single-cell transcriptomics reveals gene expression dynamics of human fetal kidney development
... Glial cell-derived neurotrophic factor/ RET proto-oncogene (GDNF/RET) signaling between the UB and CM critically regulates proliferation of UB tip cells and branching morphogenesis of the UB ...
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Comparative analysis of sequencing technologies for single cell transcriptomics
... to single-cell reaction (ground truth) (Additional file 1: Figure S1B – ...the single-cell sensitivity is computed using a logistic regres- sion model with spike-in RNA detection as a ...
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Single cell transcriptomics unveils gene regulatory network plasticity
... Comparing healthy and type 2 diabetes (T2D) cells in DE reveals an extremely high number of differentially expressed genes. Specifically, 6716 genes are differen- tially expressed with p < 0.001. A portion of these DE ...
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The identification of effectors of retinal cell fate determination through single cell transcriptomics
... ganglion cell layer (GCL) (Masland, ...retinal cell type is generated in a stereotyped timeline from a common pool of progenitors (Livesey and Cepko, 2001; Turner and Cepko, 1987; Turner et ...early-born ...
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Cell fixation and preservation for droplet-based single-cell transcriptomics
... Human Flp-In T-Rex 293 HEK cells were a gift from M. Landthaler (Max Delbrück Center for Molecular Medi- cine in the Helmholtz Association (MDC), Berlin) ori- ginally obtained from Invitrogen (catalog no. R78007); murine ...
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Single cell transcriptomics : a high resolution avenue for plant functional genomics
... with cell walls has been a challenge for the application of single- cell technologies, often resulting in low capture rates (discussed in the following ...droplet-based single-cell ...
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Exploration of the Germline Genome of the Ciliate Chilodonella uncinata through Single Cell Omics (Transcriptomics and Genomics)
... Combining single- cell transcriptomics and genomics, we have overcome these limitations and pro- vide the first insights into the structure of the germline genome of the ciliate Chilodonella uncinata, ...
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Transcriptomics of the human endometrium
... The transcriptomics of this valuable sample were compared with samples from non-pregnant patients on the same day of the cycle, highlighting a total of 394 differentially expressed genes (Van Vaerenbergh et ...
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Single cell transcriptome profiling revealed differences in gene expression during oocyte maturation in Haimen white goats.
... Juvenile in vitro embryo transfer (JIVET) was first suggested and investigated by the South Australian Research and Development Institute (Yin and Wang, 2013). JIVET is based on a biological high tech system that, ...
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Investigating cell death mechanisms in amyotrophic lateral sclerosis using transcriptomics
... Two recent studies have taken a quite different approach. Bernardini et al. (2013), concentrated upon mitochondrial related genes and Figueroa-Romero et al. (2012), tried to relate gene expression changes to methylation ...
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Advances in Swine Transcriptomics
... The past five years have seen a tremendous rise in porcine transcriptomic data. Available porcine Expressed Sequence Tags (ESTs) have expanded greatly, with over 623,000 ESTs deposited in Genbank. ESTs have been used to ...
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The utility of transcriptomics in fish conservation
... existing transcriptomics datasets or conduct meta-analyses to identify transcriptomic responses (gene groups or functional pathways) characteristic of particular stressors that can be used to develop biomarker ...
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Applying genetic test on the single cell
... After cell lysis is completed, the DNA of the cell is linearly amplified in a final volume of 60 ...of cell lysis are added 5 l of a solution at 400 M of degenerate primers (Kit gen PEPtm 75OD, ...
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Using cell type specific transcriptomics to understand how Arabidopsis roots respond to Sinorhizobium meliloti
... Plants are exposed to a wide range of micro-organisms in soils. Successful elicitation of a response requires a first establishment of communication between the plant and the micro-organism that will to interact with it, ...
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