single cells
BAIT: Organizing genomes and mapping rearrangements in single cells
... in single cells [1,2]. To generate Strand-seq data, cells are cultured with the thymidine analogue 5-bromo- 2 ′ -deoxyuridine (BrdU) for one round of DNA replica- ...dividing cells can be used ...
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BRIE: transcriptome wide splicing quantification in single cells
... within single cells, known as single-cell RNA-seq (scRNA-seq) ...between single cells at the gene level, and we know very little about the global variability of RNA splicing between ...
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Visualizing Allele Specific Expression In Single Cells
... mutant cells displaying monoallelic H19 expression, suggesting that variability in methylation may underlie the heterogeneity in allele specific ...individual cells; however, it is difficult to accurately ...
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Q&A: using Patch-seq to profile single cells
... a single cell during plasticity, or combined with transgenic, viral, and optogenetic techniques to explore the transcriptional signatures of neurons with a specific developmental lineage, neurons that project to a ...
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Visualizing mRNA Translation in situ in Single Cells
... in cells to ensure that proteins are synthesized at the right time, in the right location, and at appropriate ...levels. Single- molecule fluorescence in situ hybridization (smFISH) is a simple and widely ...
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Studies of exocytosis at single cells
... Figure 1.8 shows an example of the data obtained by monitoring exocytosis at single cells with FSCV. In this example, the potential at the electrode was scanned from -0.4 V to 1.3 V and back at 600 V/s to ...
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Sampling from single cells
... of single cells with unprecedented resolution. A single living cell can be repeatedly interrogated over time, thus giving a dynamic representation of the genotype of interest rather than a snapshot ...
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Monitoring Cl movement in single cells exposed to hypotonic solution
... This technique has been successfully used to re- cord Cl ) efflux correlated with the myogenic response in rat cerebral arteries and that found in the micro- environment of the apical lung (Doughty & Langton, 2001; ...
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How Single Cells Sense Smad3 Signal
... imaging, cells were fixed using 4% (wt/vol) paraformaldehyde for 20 min, permeabilized in 70% (vol/vol) ethanol for at least 1 d at -20 °C, and then hybridized overnight with HPLC-purified single-molecule ...
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Circuits of Dynamically Interacting Sigma Factors in Single Cells
... In bacteria, alternative sigma factors regulate diverse stress responses and developmental programs. Sigma factors share a common pool of core RNA polymerase (RNAP). Sharing has been assumed to occur through partitioning ...
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Protein fishing from single live cells
... from cells, the z-fine movement piezo was calibrated to precisely enter single cells, which was monitored through real-time measurements of perturbations in current upon cellular (cytoplasmic or ...
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Single cell transcriptome conservation in cryopreserved cells and tissues
... of single cell transcriptome variance from fresh (red) and cryopreserved (blue) cell ...between cells are displayed as principal component analysis (PCA, upper panel a-d) or t-distributed stochastic ...
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mRNA analysis of single living cells
... 10 single living cells and the results of five single cells are shown in ...single cells. In order to examine time dependent gene expression of single living cells, ...
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Efficient tumour formation by single human melanoma cells
... of single melanoma cells. Sorted cells were diluted and aliquoted into 10-ml microwells (Thermo Fisher ...containing single cells were identified by phase-contrast micro- ...identified ...
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Mechanical Modelling of single and collective cells behavior
... human cells, leading to observe a number of biological behaviors whose essential processes are often still obscure or only partially ...upon single cells is significantly influenced by the frequency ...
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Labeling of mesenchymal stem cells for MRI with single-cell sensitivity
... We chose to continue further experiments with highly labeled MSC without PS to test the biocompatibility of these labeling protocols in comparison with unlabeled cells. Assays for PDT, in vitro cell ...
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Multipotential phenotypic expression of genes encoding peptide hormones in rat insulinoma cell lines
... radioimmunoassay. We found expression of all three genes in the tumor and in the parental cell line and mixed variable phenotypes in the clonal lines derived from the parental line. We also observed the ectopic ...
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Optical and Electrical Enhancement of Organic Solar Cells.
... thin cells is the optical spacer layer (optical spacing is also one function of an exciton blocking ...to cells with thin layers. Figure 3.3 shows BHJ planar and folded cells with and without a 50 nm ...
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Leaf anatomy of the South African Danthonieae (Poaceae). IV. Merxmuellera drakensbergensis and M. stereophylla
... long cells and cork ...ostal cells: silica cells and cork cells alternate with single costal long cells throughout abaxial epidermis; all files of similar cell ...of cells ...
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Microsoft Excel (Lecture Notes)
... To copy the formatting to a single cell, several cells, or a range or ranges of cells, drag the mouse pointer across the cells or ranges of cells that you want to format. To stop forma[r] ...
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